Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YCL043C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.112 | 8.11E-11 | YDR353W | TRR1 | het | Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication |
0.110 | 1.98E-10 | YML069W | POB3 | het | Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress |
0.108 | 3.02E-10 | YKL202W_d | YKL202W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.103 | 2.09E-9 | YPL099C | AIM43 | hom | Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss |
0.103 | 2.40E-9 | YBR055C | PRP6 | het | Splicing factor, component of the U4/U6-U5 snRNP complex |
0.099 | 1.02E-8 | YOR191W | ULS1 | hom | Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress |
0.097 | 2.02E-8 | YBR189W | RPS9B | hom | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication |
0.094 | 5.30E-8 | YNR028W | CPR8 | hom | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway |
0.093 | 6.29E-8 | YGR050C_p | YGR050C_p | hom | Protein of unknown function; mRNA identified as translated by ribosome profiling data |
0.093 | 6.58E-8 | YER168C | CCA1 | het | ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites |
0.092 | 8.35E-8 | YJL076W | NET1 | het | Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication |
0.084 | 1.19E-6 | YMR117C | SPC24 | het | Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering |
0.082 | 1.82E-6 | YGR169C | PUS6 | hom | tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability |
0.081 | 2.68E-6 | YBL088C | TEL1 | hom | Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) |
0.078 | 6.00E-6 | YPR096C | YPR096C | hom | Protein of unknown function that may interact with ribosomes, based on co-purification experiments |