HIPHOP chemogenomics database

YCL043C / PDI1

Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

Zygosity: Heterozygous strain

fixedexpanded
Profile for YCL043C / PDI1

Click on Significant Values for Screen Details

Top fitness defect scores for YCL043C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2332	7982454	6.82	138.9 µM	2.10E-11		1	4.58E-12	1.14
2	SGTC_2988	9073640	6.28	71.4 µM	5.93E-10		2	1.66E-10	0.10
3	SGTC_2994	9053520	6.28	71.4 µM	6.10E-10	RPP1 & pyrimidine depletion	3	1.71E-10	1.35
4	SGTC_40	0327-0103	5.83	19.8 µM	8.11E-9		2	2.75E-9	0.58
5	SGTC_599	1935-0052	5.69	86.5 µM	1.81E-8		1	6.50E-9	0.95
6	SGTC_2832	7992762	5.28	52.0 µM	1.57E-7		3	6.62E-8	0.23
7	SGTC_1745	st081495	4.39	74.0 µM	9.98E-6	RNA processing & uracil transport	23	5.64E-6	0.03
8	SGTC_2338	7999974	4.31	200.0 µM	1.39E-5		5	8.04E-6	0.18
9	SGTC_2337	9009472	4.27	200.0 µM	1.67E-5		5	9.79E-6	0.07
10	SGTC_1713	st033146	4.09	12.8 µM	3.50E-5		8	2.16E-5	0.02
11	SGTC_2146	5545642	3.96	200.0 µM	5.82E-5		1	3.71E-5	0.26
12	SGTC_2796	7949634	3.92	45.5 µM	6.87E-5		8	4.43E-5	0.07
13	SGTC_1952	st077036	3.89	64.1 µM	7.63E-5		15	4.96E-5	0.16
14	SGTC_3315	9136395	3.87	68.5 µM	8.36E-5		9	5.47E-5	0.09
15	SGTC_1236	0330-0013	3.83	258.0 µM	9.58E-5		2	6.32E-5	0.53
16	SGTC_1038	k292-0204	3.78	154.0 µM	1.17E-4		7	7.85E-5	0.36
17	SGTC_3247	9135149	3.66	49.5 µM	1.85E-4	DNA damage response	26	1.27E-4	0.12
18	SGTC_2079	5212404	3.63	121.0 µM	2.03E-4		2	1.41E-4	0.02
19	SGTC_2895	9054447	3.55	58.4 µM	2.72E-4		2	1.92E-4	0.36
20	SGTC_1928	4'-methoxychalcone	3.54	40.3 µM	2.86E-4		9	2.03E-4	0.62

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.112	8.11E-11	YDR353W	TRR1	het	Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
0.110	1.98E-10	YML069W	POB3	het	Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
0.108	3.02E-10	YKL202W_d	YKL202W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.103	2.09E-9	YPL099C	AIM43	hom	Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.103	2.40E-9	YBR055C	PRP6	het	Splicing factor, component of the U4/U6-U5 snRNP complex
0.099	1.02E-8	YOR191W	ULS1	hom	Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.097	2.02E-8	YBR189W	RPS9B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.094	5.30E-8	YNR028W	CPR8	hom	Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
0.093	6.29E-8	YGR050C_p	YGR050C_p	hom	Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.093	6.58E-8	YER168C	CCA1	het	ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
0.092	8.35E-8	YJL076W	NET1	het	Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
0.084	1.19E-6	YMR117C	SPC24	het	Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.082	1.82E-6	YGR169C	PUS6	hom	tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
0.081	2.68E-6	YBL088C	TEL1	hom	Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.078	6.00E-6	YPR096C	YPR096C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments