YCL045C / EMC1

Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL045C / EMC1

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:6.09|P-value:3.79E-10 ID:SGTC_59|Compound:0330-0160|FD-Score:4.64|P-value:1.33E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:5.09|P-value:1.29E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.59|P-value:1.41E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.06|P-value:9.86E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:5.40|P-value:2.40E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:9.42|P-value:8.55E-22 ID:SGTC_164|Compound:4100-3994|FD-Score:3.48|P-value:2.17E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:6.50|P-value:2.56E-11 ID:SGTC_178|Compound:4092-0806|FD-Score:6.59|P-value:1.32E-11 ID:SGTC_180|Compound:1486-1293|FD-Score:3.24|P-value:5.23E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:4.79|P-value:6.43E-7 ID:SGTC_315|Compound:k915-0449|FD-Score:4.75|P-value:7.74E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.50|P-value:2.00E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:10.70|P-value:1.88E-27 ID:SGTC_568|Compound:1193-0097|FD-Score:3.08|P-value:9.19E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.11|P-value:8.32E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.16|P-value:7.44E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.65|P-value:1.19E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.08|P-value:9.02E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.67|P-value:5.06E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.43|P-value:3.73E-6 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.06|P-value:9.83E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:4.22|P-value:9.75E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.31|P-value:4.07E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.37|P-value:3.25E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.90|P-value:4.04E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.27|P-value:1.03E-13 ID:SGTC_1848|Compound:st056003|FD-Score:3.41|P-value:2.78E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.56|P-value:1.59E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.91|P-value:3.87E-5 ID:SGTC_2105|Compound:5350118|FD-Score:5.75|P-value:3.12E-9 ID:SGTC_2122|Compound:5258151|FD-Score:3.84|P-value:5.21E-5 ID:SGTC_2197|Compound:6588183|FD-Score:3.46|P-value:2.30E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.31|P-value:4.01E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.66|P-value:1.08E-4 ID:SGTC_2396|Compound:5342950|FD-Score:7.95|P-value:4.68E-16 ID:SGTC_2432|Compound:5630864|FD-Score:-3.54|P-value:1.86E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-3.23|P-value:5.72E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.49|P-value:1.40E-8 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.37|P-value:2.86E-8 ID:SGTC_2657|Compound:nystatin|FD-Score:3.27|P-value:4.71E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.26|P-value:4.83E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.07|P-value:9.24E-4 ID:SGTC_2701|Compound:st077767|FD-Score:3.53|P-value:1.74E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.80|P-value:5.91E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.06|P-value:9.64E-4 ID:SGTC_2807|Compound:7945238|FD-Score:4.00|P-value:2.54E-5 ID:SGTC_2816|Compound:7982706|FD-Score:3.50|P-value:1.95E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.10|P-value:8.57E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-4.12|P-value:1.69E-5 ID:SGTC_3081|Compound:9117243|FD-Score:3.32|P-value:3.89E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.76|P-value:7.29E-7 ID:SGTC_3093|Compound:9117344|FD-Score:3.53|P-value:1.76E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-6.64|P-value:1.12E-11 ID:SGTC_3151|Compound:9099821|FD-Score:-3.93|P-value:3.86E-5 ID:SGTC_3170|Compound:9103054|FD-Score:-3.28|P-value:4.82E-4 ID:SGTC_3282|Compound:9108498|FD-Score:5.26|P-value:5.35E-8 ID:SGTC_3285|Compound:9112818|FD-Score:4.33|P-value:6.01E-6 ID:SGTC_3289|Compound:9119912|FD-Score:3.25|P-value:5.05E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.68|P-value:1.05E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:6.09|P-value:3.79E-10 ID:SGTC_59|Compound:0330-0160|FD-Score:4.64|P-value:1.33E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:5.09|P-value:1.29E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.59|P-value:1.41E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.06|P-value:9.86E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:5.40|P-value:2.40E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:9.42|P-value:8.55E-22 ID:SGTC_164|Compound:4100-3994|FD-Score:3.48|P-value:2.17E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:6.50|P-value:2.56E-11 ID:SGTC_178|Compound:4092-0806|FD-Score:6.59|P-value:1.32E-11 ID:SGTC_180|Compound:1486-1293|FD-Score:3.24|P-value:5.23E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:4.79|P-value:6.43E-7 ID:SGTC_315|Compound:k915-0449|FD-Score:4.75|P-value:7.74E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.50|P-value:2.00E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:10.70|P-value:1.88E-27 ID:SGTC_568|Compound:1193-0097|FD-Score:3.08|P-value:9.19E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.11|P-value:8.32E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.16|P-value:7.44E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.65|P-value:1.19E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:3.08|P-value:9.02E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.67|P-value:5.06E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.43|P-value:3.73E-6 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.06|P-value:9.83E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:4.22|P-value:9.75E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.31|P-value:4.07E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.37|P-value:3.25E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.90|P-value:4.04E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.27|P-value:1.03E-13 ID:SGTC_1848|Compound:st056003|FD-Score:3.41|P-value:2.78E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.56|P-value:1.59E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.91|P-value:3.87E-5 ID:SGTC_2105|Compound:5350118|FD-Score:5.75|P-value:3.12E-9 ID:SGTC_2122|Compound:5258151|FD-Score:3.84|P-value:5.21E-5 ID:SGTC_2197|Compound:6588183|FD-Score:3.46|P-value:2.30E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.31|P-value:4.01E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.66|P-value:1.08E-4 ID:SGTC_2396|Compound:5342950|FD-Score:7.95|P-value:4.68E-16 ID:SGTC_2432|Compound:5630864|FD-Score:-3.54|P-value:1.86E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-3.23|P-value:5.72E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.49|P-value:1.40E-8 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.37|P-value:2.86E-8 ID:SGTC_2657|Compound:nystatin|FD-Score:3.27|P-value:4.71E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.26|P-value:4.83E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.07|P-value:9.24E-4 ID:SGTC_2701|Compound:st077767|FD-Score:3.53|P-value:1.74E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.80|P-value:5.91E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.06|P-value:9.64E-4 ID:SGTC_2807|Compound:7945238|FD-Score:4.00|P-value:2.54E-5 ID:SGTC_2816|Compound:7982706|FD-Score:3.50|P-value:1.95E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.10|P-value:8.57E-4 ID:SGTC_2917|Compound:7979103|FD-Score:-4.12|P-value:1.69E-5 ID:SGTC_3081|Compound:9117243|FD-Score:3.32|P-value:3.89E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.76|P-value:7.29E-7 ID:SGTC_3093|Compound:9117344|FD-Score:3.53|P-value:1.76E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-6.64|P-value:1.12E-11 ID:SGTC_3151|Compound:9099821|FD-Score:-3.93|P-value:3.86E-5 ID:SGTC_3170|Compound:9103054|FD-Score:-3.28|P-value:4.82E-4 ID:SGTC_3282|Compound:9108498|FD-Score:5.26|P-value:5.35E-8 ID:SGTC_3285|Compound:9112818|FD-Score:4.33|P-value:6.01E-6 ID:SGTC_3289|Compound:9119912|FD-Score:3.25|P-value:5.05E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.68|P-value:1.05E-4

Top fitness defect scores for YCL045C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_562 3772-2733 10.70 112.0 µM 1.88E-27 ERG2 8 6.10E-27
2 SGTC_163 1327-0036 9.42 7.5 µM 8.55E-22 15 2.17E-21
3 SGTC_2396 5342950 7.95 42.8 µM 4.68E-16 RSC complex & mRNA processing 5 9.22E-16
4 SGTC_1750 st081859 7.27 2.4 µM 1.03E-13 endomembrane recycling 83 1.84E-13
5 SGTC_178 4092-0806 6.59 87.5 µM 1.32E-11 ergosterol depletion effects on membrane 31 2.14E-11
6 SGTC_176 1486-1328 6.50 72.8 µM 2.56E-11 16 4.09E-11
7 SGTC_43 0570-0105 6.09 72.5 µM 3.79E-10 ERG2 36 5.75E-10
8 SGTC_2105 5350118 5.75 200.0 µM 3.12E-9 6 4.55E-9
9 SGTC_1202 1988-0068 5.67 29.6 µM 5.06E-9 RNA processing & uracil transport 8 7.30E-9
10 SGTC_2645 abamectin 5.49 100.0 µM 1.40E-8 heme requiring 13 1.98E-8
11 SGTC_142 0335-0849 5.40 62.7 µM 2.40E-8 ubiquinone biosynthesis & proteasome 77 3.36E-8
12 SGTC_2651 1327-0036 5.37 5.0 µM 2.86E-8 94 4.00E-8
13 SGTC_3282 9108498 5.26 15.5 µM 5.35E-8 12 7.38E-8
14 SGTC_64 000l-0018 5.09 272.1 µM 1.29E-7 ubiquinone biosynthesis & proteasome 105 1.75E-7
15 SGTC_188 3229-0966 4.79 278.5 µM 6.43E-7 17 8.45E-7
16 SGTC_3087 9117406 4.76 49.5 µM 7.29E-7 5 9.55E-7
17 SGTC_315 k915-0449 4.75 187.0 µM 7.74E-7 37 1.01E-6
18 SGTC_59 0330-0160 4.64 1.3 µM 1.33E-6 78 1.72E-6
19 SGTC_1259 0671-0132 4.43 43.0 µM 3.73E-6 15 4.73E-6
20 SGTC_3285 9112818 4.33 20.9 µM 6.01E-6 cell wall 25 7.55E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.320 1.31E-80 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.314 1.07E-77 YKL207W EMC3 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
0.296 6.28E-69 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.220 7.46E-38 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.207 1.16E-33 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.205 3.14E-33 YLL014W EMC6 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
0.205 2.87E-33 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.202 2.50E-32 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.194 1.07E-29 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.193 1.66E-29 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.191 4.57E-29 YBR106W PHO88 hom Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
0.181 3.47E-26 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.180 7.14E-26 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.176 6.92E-25 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.174 3.82E-24 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway