YCL054W / SPB1

AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCL054W / SPB1

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:3.49|P-value:5.32E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.27|P-value:3.15E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.04|P-value:7.73E-5 ID:SGTC_191|Compound:3914-0123|FD-Score:3.54|P-value:4.57E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.55|P-value:4.44E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.56|P-value:4.24E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.32|P-value:9.05E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:-3.45|P-value:6.00E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:3.63|P-value:3.35E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.27|P-value:3.14E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.96|P-value:1.02E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.39|P-value:7.55E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-3.36|P-value:8.01E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.75|P-value:4.30E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:3.52|P-value:4.83E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:-3.48|P-value:5.39E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.30|P-value:9.92E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.31|P-value:9.76E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.02|P-value:1.25E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:4.32|P-value:2.58E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.50|P-value:5.26E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.97|P-value:1.01E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.59|P-value:3.82E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-4.10|P-value:5.78E-5 ID:SGTC_1678|Compound:st019265|FD-Score:3.72|P-value:2.46E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.35|P-value:8.07E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.79|P-value:1.84E-4 ID:SGTC_1761|Compound:st044296|FD-Score:4.86|P-value:2.64E-6 ID:SGTC_1974|Compound:st069448|FD-Score:-3.71|P-value:2.48E-4 ID:SGTC_1976|Compound:st070609|FD-Score:-3.50|P-value:4.99E-4 ID:SGTC_2173|Compound:5763948|FD-Score:4.53|P-value:1.08E-5 ID:SGTC_2192|Compound:5915882|FD-Score:4.70|P-value:5.20E-6 ID:SGTC_2270|Compound:7924575|FD-Score:3.62|P-value:3.43E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.54|P-value:4.32E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-4.99|P-value:1.40E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.73|P-value:4.48E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.33|P-value:8.71E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.33|P-value:8.81E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.46|P-value:5.82E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.38|P-value:1.96E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.42|P-value:6.68E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.34|P-value:8.76E-4 ID:SGTC_2825|Compound:7998094|FD-Score:7.17|P-value:8.83E-12 ID:SGTC_2867|Compound:9039098|FD-Score:3.84|P-value:1.59E-4 ID:SGTC_2922|Compound:7999257|FD-Score:5.23|P-value:4.80E-7 ID:SGTC_2923|Compound:7992654|FD-Score:3.64|P-value:3.23E-4 ID:SGTC_2928|Compound:9011484|FD-Score:-3.32|P-value:8.92E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.73|P-value:2.37E-4 ID:SGTC_2975|Compound:9084847|FD-Score:5.22|P-value:4.83E-7 ID:SGTC_3023|Compound:9089636|FD-Score:3.41|P-value:7.05E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.58|P-value:3.79E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.38|P-value:7.48E-4 ID:SGTC_3152|Compound:9099253|FD-Score:9.54|P-value:1.85E-19 ID:SGTC_3159|Compound:9100096|FD-Score:4.79|P-value:3.50E-6 ID:SGTC_3200|Compound:9114385|FD-Score:-4.67|P-value:5.70E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:3.49|P-value:5.32E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.27|P-value:3.15E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.04|P-value:7.73E-5 ID:SGTC_191|Compound:3914-0123|FD-Score:3.54|P-value:4.57E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.55|P-value:4.44E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.56|P-value:4.24E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.32|P-value:9.05E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:-3.45|P-value:6.00E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:3.63|P-value:3.35E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.27|P-value:3.14E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.96|P-value:1.02E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.39|P-value:7.55E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-3.36|P-value:8.01E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.75|P-value:4.30E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:3.52|P-value:4.83E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:-3.48|P-value:5.39E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.30|P-value:9.92E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.31|P-value:9.76E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:5.02|P-value:1.25E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:4.32|P-value:2.58E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.50|P-value:5.26E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.97|P-value:1.01E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.59|P-value:3.82E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-4.10|P-value:5.78E-5 ID:SGTC_1678|Compound:st019265|FD-Score:3.72|P-value:2.46E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.35|P-value:8.07E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.79|P-value:1.84E-4 ID:SGTC_1761|Compound:st044296|FD-Score:4.86|P-value:2.64E-6 ID:SGTC_1974|Compound:st069448|FD-Score:-3.71|P-value:2.48E-4 ID:SGTC_1976|Compound:st070609|FD-Score:-3.50|P-value:4.99E-4 ID:SGTC_2173|Compound:5763948|FD-Score:4.53|P-value:1.08E-5 ID:SGTC_2192|Compound:5915882|FD-Score:4.70|P-value:5.20E-6 ID:SGTC_2270|Compound:7924575|FD-Score:3.62|P-value:3.43E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.54|P-value:4.32E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-4.99|P-value:1.40E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.73|P-value:4.48E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.33|P-value:8.71E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.33|P-value:8.81E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.46|P-value:5.82E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.38|P-value:1.96E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.42|P-value:6.68E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.34|P-value:8.76E-4 ID:SGTC_2825|Compound:7998094|FD-Score:7.17|P-value:8.83E-12 ID:SGTC_2867|Compound:9039098|FD-Score:3.84|P-value:1.59E-4 ID:SGTC_2922|Compound:7999257|FD-Score:5.23|P-value:4.80E-7 ID:SGTC_2923|Compound:7992654|FD-Score:3.64|P-value:3.23E-4 ID:SGTC_2928|Compound:9011484|FD-Score:-3.32|P-value:8.92E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.73|P-value:2.37E-4 ID:SGTC_2975|Compound:9084847|FD-Score:5.22|P-value:4.83E-7 ID:SGTC_3023|Compound:9089636|FD-Score:3.41|P-value:7.05E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.58|P-value:3.79E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.38|P-value:7.48E-4 ID:SGTC_3152|Compound:9099253|FD-Score:9.54|P-value:1.85E-19 ID:SGTC_3159|Compound:9100096|FD-Score:4.79|P-value:3.50E-6 ID:SGTC_3200|Compound:9114385|FD-Score:-4.67|P-value:5.70E-6

Top fitness defect scores for YCL054W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3152 9099253 9.54 49.5 µM 1.85E-19 NEO1-PIK1 5 7.30E-22 0.50
2 SGTC_2825 7998094 7.17 58.4 µM 8.83E-12 fatty acid desaturase (OLE1) 3 3.74E-13 1.74
3 SGTC_2922 7999257 5.23 32.5 µM 4.80E-7 2 8.65E-8 0.69
4 SGTC_2975 9084847 5.22 53.7 µM 4.83E-7 1 8.72E-8 0.75
5 SGTC_1051 3253-3903 5.02 78.0 µM 1.25E-6 3 2.55E-7 1.02
6 SGTC_1761 st044296 4.86 37.0 µM 2.64E-6 3 5.95E-7 0.76
7 SGTC_3159 9100096 4.79 49.5 µM 3.50E-6 2 8.18E-7 0.36
8 SGTC_800 3448-7362 4.75 155.0 µM 4.30E-6 translation 36 1.03E-6 0.10
9 SGTC_2192 5915882 4.70 198.5 µM 5.20E-6 RNA processing & uracil transport 11 1.28E-6 0.11
10 SGTC_2173 5763948 4.53 167.0 µM 1.08E-5 tubulin folding & SWR complex 2 2.92E-6 0.17
11 SGTC_1074 carmofur 4.32 3.8 µM 2.58E-5 exosome 24 7.85E-6 0.11
12 SGTC_580 1123-0028 4.27 41.0 µM 3.14E-5 DNA damage response 18 9.77E-6 0.14
13 SGTC_136 0097-0015 4.27 404.5 µM 3.15E-5 60S ribosome export 8 9.81E-6 0.19
14 SGTC_172 1112-0046 4.04 2.6 µM 7.73E-5 NEO1 9 2.71E-5 0.22
15 SGTC_1465 k213-0056 3.97 144.0 µM 1.01E-4 DNA intercalators 11 3.65E-5 0.05
16 SGTC_682 0kpi-0099 3.96 17.0 µM 1.02E-4 29 3.68E-5 0.13
17 SGTC_2867 9039098 3.84 71.4 µM 1.59E-4 iron homeostasis 15 6.10E-5 0.22
18 SGTC_2966 9084041 3.73 48.0 µM 2.37E-4 4 9.57E-5 0.12
19 SGTC_1678 st019265 3.72 80.2 µM 2.46E-4 60S ribosome export 4 9.99E-5 0.02
20 SGTC_2923 7992654 3.64 58.1 µM 3.23E-4 Golgi 6 1.36E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 5.73E-12 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.115 2.59E-11 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.115 2.73E-11 YJR126C VPS70 hom Protein of unknown function involved in vacuolar protein sorting
0.113 4.92E-11 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.112 9.09E-11 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.107 4.63E-10 YHR070W TRM5 het tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya
0.104 1.36E-9 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.097 1.92E-8 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.095 3.53E-8 YGR282C BGL2 hom Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance
0.095 3.98E-8 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.094 4.97E-8 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.093 7.45E-8 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.093 7.78E-8 YPR137W RRP9 het Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
0.093 7.95E-8 YLR320W MMS22 hom Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
0.092 8.16E-8 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis