YCL057W / PRD1

Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL057W / PRD1

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:3.63|P-value:2.21E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.34|P-value:8.86E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.23|P-value:8.42E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.37|P-value:8.04E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:4.19|P-value:2.58E-5 ID:SGTC_720|Compound:r052-1644|FD-Score:4.90|P-value:1.15E-6 ID:SGTC_827|Compound:3253-0474|FD-Score:3.85|P-value:9.74E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.39|P-value:4.95E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.75|P-value:1.40E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.53|P-value:4.66E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.57|P-value:5.05E-6 ID:SGTC_912|Compound:3474-0017|FD-Score:3.86|P-value:9.48E-5 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:4.60|P-value:4.40E-6 ID:SGTC_1076|Compound:itraconazole|FD-Score:4.37|P-value:1.23E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.50|P-value:3.42E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.20|P-value:9.46E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.12|P-value:3.88E-7 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.68|P-value:2.76E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.32|P-value:9.30E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.84|P-value:1.01E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.86|P-value:9.21E-5 ID:SGTC_1764|Compound:st044502|FD-Score:5.57|P-value:4.00E-8 ID:SGTC_1898|Compound:st060199|FD-Score:-3.92|P-value:1.16E-4 ID:SGTC_1903|Compound:st060211|FD-Score:4.81|P-value:1.70E-6 ID:SGTC_2059|Compound:5238311|FD-Score:5.01|P-value:6.61E-7 ID:SGTC_2133|Compound:5320837|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_2188|Compound:6126565|FD-Score:-3.32|P-value:9.51E-4 ID:SGTC_2196|Compound:6573537|FD-Score:4.77|P-value:2.07E-6 ID:SGTC_2203|Compound:7227154|FD-Score:-3.66|P-value:3.02E-4 ID:SGTC_2225|Compound:6654152|FD-Score:-3.91|P-value:1.23E-4 ID:SGTC_2411|Compound:st077133|FD-Score:-6.26|P-value:1.70E-9 ID:SGTC_2413|Compound:inermin|FD-Score:-3.32|P-value:9.31E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.64|P-value:3.66E-6 ID:SGTC_2468|Compound:5987765|FD-Score:3.94|P-value:6.74E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.53|P-value:3.06E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.87|P-value:8.93E-5 ID:SGTC_2877|Compound:9040724|FD-Score:3.21|P-value:8.99E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.23|P-value:8.54E-4 ID:SGTC_3120|Compound:9124077|FD-Score:4.15|P-value:3.00E-5 ID:SGTC_3150|Compound:9097574|FD-Score:3.53|P-value:3.08E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.60|P-value:3.73E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.99|P-value:5.76E-5 ID:SGTC_3217|Compound:9129673|FD-Score:-3.64|P-value:3.26E-4 ID:SGTC_3228|Compound:9131799|FD-Score:4.16|P-value:2.86E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-5.17|P-value:5.69E-7 ID:SGTC_49|Compound:0717-0965|FD-Score:3.63|P-value:2.21E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.34|P-value:8.86E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.23|P-value:8.42E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.37|P-value:8.04E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.42|P-value:4.62E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:4.19|P-value:2.58E-5 ID:SGTC_720|Compound:r052-1644|FD-Score:4.90|P-value:1.15E-6 ID:SGTC_827|Compound:3253-0474|FD-Score:3.85|P-value:9.74E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:3.39|P-value:4.95E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.75|P-value:1.40E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.53|P-value:4.66E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.57|P-value:5.05E-6 ID:SGTC_912|Compound:3474-0017|FD-Score:3.86|P-value:9.48E-5 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:4.60|P-value:4.40E-6 ID:SGTC_1076|Compound:itraconazole|FD-Score:4.37|P-value:1.23E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.50|P-value:3.42E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.20|P-value:9.46E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.12|P-value:3.88E-7 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.68|P-value:2.76E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.32|P-value:9.30E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.84|P-value:1.01E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.86|P-value:9.21E-5 ID:SGTC_1764|Compound:st044502|FD-Score:5.57|P-value:4.00E-8 ID:SGTC_1898|Compound:st060199|FD-Score:-3.92|P-value:1.16E-4 ID:SGTC_1903|Compound:st060211|FD-Score:4.81|P-value:1.70E-6 ID:SGTC_2059|Compound:5238311|FD-Score:5.01|P-value:6.61E-7 ID:SGTC_2133|Compound:5320837|FD-Score:3.54|P-value:2.97E-4 ID:SGTC_2188|Compound:6126565|FD-Score:-3.32|P-value:9.51E-4 ID:SGTC_2196|Compound:6573537|FD-Score:4.77|P-value:2.07E-6 ID:SGTC_2203|Compound:7227154|FD-Score:-3.66|P-value:3.02E-4 ID:SGTC_2225|Compound:6654152|FD-Score:-3.91|P-value:1.23E-4 ID:SGTC_2411|Compound:st077133|FD-Score:-6.26|P-value:1.70E-9 ID:SGTC_2413|Compound:inermin|FD-Score:-3.32|P-value:9.31E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.64|P-value:3.66E-6 ID:SGTC_2468|Compound:5987765|FD-Score:3.94|P-value:6.74E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.53|P-value:3.06E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.87|P-value:8.93E-5 ID:SGTC_2877|Compound:9040724|FD-Score:3.21|P-value:8.99E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.23|P-value:8.54E-4 ID:SGTC_3120|Compound:9124077|FD-Score:4.15|P-value:3.00E-5 ID:SGTC_3150|Compound:9097574|FD-Score:3.53|P-value:3.08E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.60|P-value:3.73E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.99|P-value:5.76E-5 ID:SGTC_3217|Compound:9129673|FD-Score:-3.64|P-value:3.26E-4 ID:SGTC_3228|Compound:9131799|FD-Score:4.16|P-value:2.86E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-5.17|P-value:5.69E-7

Top fitness defect scores for YCL057W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1764 st044502 5.57 93.5 µM 4.00E-8 9 1.27E-8
2 SGTC_1308 1187-1617 5.12 41.4 µM 3.88E-7 fatty acid desaturase (OLE1) 17 1.50E-7
3 SGTC_2059 5238311 5.01 157.0 µM 6.61E-7 6 2.67E-7
4 SGTC_720 r052-1644 4.90 49.9 µM 1.15E-6 TSC3-RPN4 15 4.86E-7
5 SGTC_1903 st060211 4.81 78.3 µM 1.70E-6 10 7.42E-7
6 SGTC_2196 6573537 4.77 42.7 µM 2.07E-6 copper-dependent oxidative stress 4 9.22E-7
7 SGTC_2417 cerulenin 4.64 830.0 nM 3.66E-6 17 1.71E-6
8 SGTC_1069 diphenylcyclopropenone 4.60 8.5 µM 4.40E-6 20 2.09E-6
9 SGTC_893 0958-0369 4.57 48.9 µM 5.05E-6 17 2.42E-6
10 SGTC_1076 itraconazole 4.37 331.0 nM 1.23E-5 23 6.35E-6
11 SGTC_712 r015-0009 4.19 80.6 µM 2.58E-5 22 1.42E-5
12 SGTC_3228 9131799 4.16 49.5 µM 2.86E-5 amide catabolism 4 1.58E-5
13 SGTC_3120 9124077 4.15 49.5 µM 3.00E-5 17 1.66E-5
14 SGTC_3200 9114385 3.99 49.5 µM 5.76E-5 33 3.37E-5
15 SGTC_2468 5987765 3.94 72.9 µM 6.74E-5 14 4.00E-5
16 SGTC_2583 asiatic acid 3.87 100.0 µM 8.93E-5 39 5.42E-5
17 SGTC_1605 st000082 3.86 49.1 µM 9.21E-5 60S ribosome export 17 5.60E-5
18 SGTC_912 3474-0017 3.86 2.2 µM 9.48E-5 25 5.78E-5
19 SGTC_827 3253-0474 3.85 693.0 µM 9.74E-5 22 5.95E-5
20 SGTC_1363 1636-0063 3.84 10.4 µM 1.01E-4 Golgi 31 6.17E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 5.92E-17 YOR342C YOR342C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
0.119 4.65E-12 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.107 4.25E-10 YML094W GIM5 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.103 1.92E-9 YGR039W_d YGR039W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722
0.098 1.18E-8 YJR042W NUP85 het Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
0.096 2.31E-8 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.094 4.46E-8 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.086 6.81E-7 YNL071W LAT1 hom Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
0.085 9.35E-7 YOR011W AUS1 hom Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen
0.082 2.17E-6 YNL193W_p YNL193W_p hom Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
0.081 2.59E-6 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.078 6.32E-6 YDR077W SED1 hom Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites
0.074 1.81E-5 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.072 2.71E-5 YHR093W_d AHT1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region
0.072 3.35E-5 YHR132C ECM14 hom Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly