YCL061C / MRC1

S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL061C / MRC1

Click on Significant Values for Screen Details ID:SGTC_121|Compound:0557-0495|FD-Score:4.15|P-value:7.30E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:3.91|P-value:1.74E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.00|P-value:1.26E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.73|P-value:3.20E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.91|P-value:1.60E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:4.00|P-value:1.25E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.57|P-value:1.26E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.86|P-value:2.03E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:7.54|P-value:2.33E-12 ID:SGTC_671|Compound:0560-0003|FD-Score:3.40|P-value:9.18E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.71|P-value:3.46E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.99|P-value:1.20E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:-6.12|P-value:9.00E-9 ID:SGTC_727|Compound:3032-0951|FD-Score:3.47|P-value:7.43E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.76|P-value:6.54E-6 ID:SGTC_916|Compound:4466-0038|FD-Score:5.60|P-value:1.40E-7 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.86|P-value:2.04E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.82|P-value:2.18E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.98|P-value:1.35E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.76|P-value:2.89E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.93|P-value:3.07E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.81|P-value:2.44E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.54|P-value:6.04E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.61|P-value:4.74E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.87|P-value:1.99E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:4.98|P-value:2.49E-6 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:6.57E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.50|P-value:6.75E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.73|P-value:2.96E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.77|P-value:2.80E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.54|P-value:6.02E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.95|P-value:1.48E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.95|P-value:1.51E-4 ID:SGTC_1925|Compound:st057770|FD-Score:4.20|P-value:6.04E-5 ID:SGTC_1930|Compound:st074634|FD-Score:4.59|P-value:1.33E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.51|P-value:6.48E-4 ID:SGTC_2011|Compound:4012611|FD-Score:4.27|P-value:4.65E-5 ID:SGTC_2112|Compound:5488546|FD-Score:4.16|P-value:6.99E-5 ID:SGTC_2124|Compound:5246320|FD-Score:6.63|P-value:6.18E-10 ID:SGTC_2132|Compound:5317594|FD-Score:4.10|P-value:8.60E-5 ID:SGTC_2258|Compound:7960471|FD-Score:4.51|P-value:1.82E-5 ID:SGTC_2281|Compound:7943571|FD-Score:4.19|P-value:6.18E-5 ID:SGTC_2283|Compound:7944793|FD-Score:6.43|P-value:1.85E-9 ID:SGTC_2289|Compound:7946404|FD-Score:4.54|P-value:1.63E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.47|P-value:7.46E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.50|P-value:6.73E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-3.49|P-value:6.39E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.49|P-value:7.01E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.44|P-value:2.40E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:10.10|P-value:6.59E-21 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-4.04|P-value:9.92E-5 ID:SGTC_2686|Compound:alizarin|FD-Score:5.86|P-value:3.83E-8 ID:SGTC_2791|Compound:5663188|FD-Score:3.47|P-value:7.50E-4 ID:SGTC_2867|Compound:9039098|FD-Score:5.05|P-value:1.89E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-4.41|P-value:2.43E-5 ID:SGTC_2927|Compound:9011802|FD-Score:4.80|P-value:5.58E-6 ID:SGTC_3066|Compound:9117245|FD-Score:3.87|P-value:2.00E-4 ID:SGTC_3129|Compound:9127257|FD-Score:7.01|P-value:6.60E-11 ID:SGTC_3242|Compound:9133940|FD-Score:3.43|P-value:8.37E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.89|P-value:1.83E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.45|P-value:7.94E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.15|P-value:7.30E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:3.91|P-value:1.74E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.00|P-value:1.26E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.73|P-value:3.20E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.91|P-value:1.60E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:4.00|P-value:1.25E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.57|P-value:1.26E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.86|P-value:2.03E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:7.54|P-value:2.33E-12 ID:SGTC_671|Compound:0560-0003|FD-Score:3.40|P-value:9.18E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.71|P-value:3.46E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.99|P-value:1.20E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:-6.12|P-value:9.00E-9 ID:SGTC_727|Compound:3032-0951|FD-Score:3.47|P-value:7.43E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.76|P-value:6.54E-6 ID:SGTC_916|Compound:4466-0038|FD-Score:5.60|P-value:1.40E-7 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.86|P-value:2.04E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.82|P-value:2.18E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.98|P-value:1.35E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.76|P-value:2.89E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.93|P-value:3.07E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.81|P-value:2.44E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.54|P-value:6.04E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.61|P-value:4.74E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.87|P-value:1.99E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:4.98|P-value:2.49E-6 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:6.57E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.50|P-value:6.75E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.73|P-value:2.96E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.77|P-value:2.80E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.54|P-value:6.02E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.95|P-value:1.48E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.95|P-value:1.51E-4 ID:SGTC_1925|Compound:st057770|FD-Score:4.20|P-value:6.04E-5 ID:SGTC_1930|Compound:st074634|FD-Score:4.59|P-value:1.33E-5 ID:SGTC_1939|Compound:st074455|FD-Score:3.51|P-value:6.48E-4 ID:SGTC_2011|Compound:4012611|FD-Score:4.27|P-value:4.65E-5 ID:SGTC_2112|Compound:5488546|FD-Score:4.16|P-value:6.99E-5 ID:SGTC_2124|Compound:5246320|FD-Score:6.63|P-value:6.18E-10 ID:SGTC_2132|Compound:5317594|FD-Score:4.10|P-value:8.60E-5 ID:SGTC_2258|Compound:7960471|FD-Score:4.51|P-value:1.82E-5 ID:SGTC_2281|Compound:7943571|FD-Score:4.19|P-value:6.18E-5 ID:SGTC_2283|Compound:7944793|FD-Score:6.43|P-value:1.85E-9 ID:SGTC_2289|Compound:7946404|FD-Score:4.54|P-value:1.63E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.47|P-value:7.46E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.50|P-value:6.73E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-3.49|P-value:6.39E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.49|P-value:7.01E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.44|P-value:2.40E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:10.10|P-value:6.59E-21 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-4.04|P-value:9.92E-5 ID:SGTC_2686|Compound:alizarin|FD-Score:5.86|P-value:3.83E-8 ID:SGTC_2791|Compound:5663188|FD-Score:3.47|P-value:7.50E-4 ID:SGTC_2867|Compound:9039098|FD-Score:5.05|P-value:1.89E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-4.41|P-value:2.43E-5 ID:SGTC_2927|Compound:9011802|FD-Score:4.80|P-value:5.58E-6 ID:SGTC_3066|Compound:9117245|FD-Score:3.87|P-value:2.00E-4 ID:SGTC_3129|Compound:9127257|FD-Score:7.01|P-value:6.60E-11 ID:SGTC_3242|Compound:9133940|FD-Score:3.43|P-value:8.37E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.89|P-value:1.83E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.45|P-value:7.94E-4

Top fitness defect scores for YCL061C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2619 obliquin 10.10 87.9 µM 6.59E-21 cell wall 21 1.75E-24
2 SGTC_514 4466-0038 7.54 2.6 nM 2.33E-12 cell wall 49 2.33E-14
3 SGTC_3129 9127257 7.01 49.5 µM 6.60E-11 2 1.22E-12
4 SGTC_2124 5246320 6.63 200.0 µM 6.18E-10 heme biosynthesis & mitochondrial translocase 2 1.71E-11
5 SGTC_2283 7944793 6.43 200.0 µM 1.85E-9 4 6.26E-11
6 SGTC_2686 alizarin 5.86 95.4 µM 3.83E-8 RPP1 & pyrimidine depletion 7 2.25E-9
7 SGTC_916 4466-0038 5.60 42.0 nM 1.40E-7 cell wall 30 1.04E-8
8 SGTC_2867 9039098 5.05 71.4 µM 1.89E-6 iron homeostasis 11 2.26E-7
9 SGTC_1407 3909-7909 4.98 12.3 µM 2.49E-6 60S ribosome export 5 3.14E-7
10 SGTC_1049 3226-0134 4.93 66.0 µM 3.07E-6 11 4.01E-7
11 SGTC_2927 9011802 4.80 47.9 µM 5.58E-6 13 8.11E-7
12 SGTC_881 0833-0569 4.76 170.0 µM 6.54E-6 9 9.79E-7
13 SGTC_1930 st074634 4.59 34.3 µM 1.33E-5 RNA pol III & RNase P/MRP 22 2.25E-6
14 SGTC_2289 7946404 4.54 165.1 µM 1.63E-5 73 2.88E-6
15 SGTC_2258 7960471 4.51 200.0 µM 1.82E-5 5 3.26E-6
16 SGTC_2557 derrustone 4.44 83.8 µM 2.40E-5 21 4.55E-6
17 SGTC_2011 4012611 4.27 177.0 µM 4.65E-5 mitochondrial processes 22 9.89E-6
18 SGTC_1925 st057770 4.20 10.1 µM 6.04E-5 TSC3-RPN4 17 1.35E-5
19 SGTC_2281 7943571 4.19 200.0 µM 6.18E-5 7 1.38E-5
20 SGTC_2112 5488546 4.16 200.0 µM 6.99E-5 TSC3-RPN4 15 1.60E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.266 1.83E-55 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.214 3.53E-36 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.207 9.31E-34 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.201 5.17E-32 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.179 1.23E-25 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.168 9.82E-23 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.160 1.14E-20 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.158 4.55E-20 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.155 1.36E-19 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.154 2.80E-19 YPR120C CLB5 hom B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase
0.152 8.78E-19 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.149 3.10E-18 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.149 3.21E-18 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.148 6.21E-18 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.148 7.71E-18 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization