YCR003W / MRPL32

Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR003W / MRPL32

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.61|P-value:5.18E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.55|P-value:6.44E-5 ID:SGTC_33|Compound:0986-0033|FD-Score:6.34|P-value:5.12E-8 ID:SGTC_39|Compound:0986-0283|FD-Score:5.51|P-value:1.85E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:5.11|P-value:8.76E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.19|P-value:2.11E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.55|P-value:1.60E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:13.70|P-value:1.08E-30 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.80|P-value:6.89E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.52|P-value:2.19E-8 ID:SGTC_386|Compound:1509-0011|FD-Score:6.05|P-value:1.90E-7 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.14|P-value:3.19E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:5.28|P-value:4.62E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.81|P-value:7.59E-7 ID:SGTC_456|Compound:gingerol|FD-Score:4.90|P-value:1.90E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.59|P-value:7.46E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:7.85|P-value:2.32E-11 ID:SGTC_648|Compound:0375-0473|FD-Score:3.84|P-value:5.99E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:4.21|P-value:1.95E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.44|P-value:9.35E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.80|P-value:8.64E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.77|P-value:9.32E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.89|P-value:5.25E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.85|P-value:2.23E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.60|P-value:5.40E-5 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.64|P-value:4.66E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.19|P-value:2.10E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.13|P-value:7.96E-6 ID:SGTC_1658|Compound:st012944|FD-Score:-3.94|P-value:5.85E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-4.20|P-value:2.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.52|P-value:9.38E-5 ID:SGTC_1742|Compound:st038235|FD-Score:8.72|P-value:1.42E-13 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.62|P-value:1.66E-6 ID:SGTC_1791|Compound:st048313|FD-Score:3.95|P-value:4.36E-4 ID:SGTC_1812|Compound:st052057|FD-Score:7.01|P-value:2.11E-9 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:4.26|P-value:1.69E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.91|P-value:4.96E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.40|P-value:2.88E-6 ID:SGTC_1941|Compound:st076221|FD-Score:-4.69|P-value:5.28E-5 ID:SGTC_1950|Compound:st077106|FD-Score:-4.15|P-value:3.07E-4 ID:SGTC_2087|Compound:5276107|FD-Score:4.14|P-value:2.46E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:-3.93|P-value:6.03E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.88|P-value:3.92E-7 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.68|P-value:9.69E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.66|P-value:9.79E-7 ID:SGTC_2278|Compound:7948257|FD-Score:4.10|P-value:2.76E-4 ID:SGTC_2283|Compound:7944793|FD-Score:5.55|P-value:1.55E-6 ID:SGTC_2414|Compound:st077232|FD-Score:-3.89|P-value:6.69E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-5.12|P-value:1.17E-5 ID:SGTC_2449|Compound:5812982|FD-Score:-6.52|P-value:3.34E-8 ID:SGTC_2479|Compound:5617353|FD-Score:-4.08|P-value:3.79E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-5.09|P-value:1.30E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-5.09|P-value:1.29E-5 ID:SGTC_2488|Compound:5268135|FD-Score:5.77|P-value:6.33E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:6.65|P-value:1.21E-8 ID:SGTC_2570|Compound:licareol|FD-Score:6.43|P-value:3.42E-8 ID:SGTC_2691|Compound:st077356|FD-Score:4.61|P-value:5.34E-5 ID:SGTC_2727|Compound:riluzole|FD-Score:3.85|P-value:5.79E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.88|P-value:6.80E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.12|P-value:2.05E-7 ID:SGTC_2903|Compound:5923464|FD-Score:3.67|P-value:9.77E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.78|P-value:7.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.37|P-value:1.56E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-4.11|P-value:3.44E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.85|P-value:7.42E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-3.86|P-value:7.32E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.61|P-value:5.18E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.55|P-value:6.44E-5 ID:SGTC_33|Compound:0986-0033|FD-Score:6.34|P-value:5.12E-8 ID:SGTC_39|Compound:0986-0283|FD-Score:5.51|P-value:1.85E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:5.11|P-value:8.76E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.19|P-value:2.11E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.55|P-value:1.60E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:13.70|P-value:1.08E-30 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.80|P-value:6.89E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.52|P-value:2.19E-8 ID:SGTC_386|Compound:1509-0011|FD-Score:6.05|P-value:1.90E-7 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.14|P-value:3.19E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:5.28|P-value:4.62E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.81|P-value:7.59E-7 ID:SGTC_456|Compound:gingerol|FD-Score:4.90|P-value:1.90E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.59|P-value:7.46E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:7.85|P-value:2.32E-11 ID:SGTC_648|Compound:0375-0473|FD-Score:3.84|P-value:5.99E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:4.21|P-value:1.95E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.44|P-value:9.35E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.80|P-value:8.64E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.77|P-value:9.32E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.89|P-value:5.25E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.85|P-value:2.23E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.60|P-value:5.40E-5 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.64|P-value:4.66E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.19|P-value:2.10E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.13|P-value:7.96E-6 ID:SGTC_1658|Compound:st012944|FD-Score:-3.94|P-value:5.85E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-4.20|P-value:2.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.52|P-value:9.38E-5 ID:SGTC_1742|Compound:st038235|FD-Score:8.72|P-value:1.42E-13 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.62|P-value:1.66E-6 ID:SGTC_1791|Compound:st048313|FD-Score:3.95|P-value:4.36E-4 ID:SGTC_1812|Compound:st052057|FD-Score:7.01|P-value:2.11E-9 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:4.26|P-value:1.69E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.91|P-value:4.96E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.40|P-value:2.88E-6 ID:SGTC_1941|Compound:st076221|FD-Score:-4.69|P-value:5.28E-5 ID:SGTC_1950|Compound:st077106|FD-Score:-4.15|P-value:3.07E-4 ID:SGTC_2087|Compound:5276107|FD-Score:4.14|P-value:2.46E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:-3.93|P-value:6.03E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.88|P-value:3.92E-7 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.68|P-value:9.69E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.66|P-value:9.79E-7 ID:SGTC_2278|Compound:7948257|FD-Score:4.10|P-value:2.76E-4 ID:SGTC_2283|Compound:7944793|FD-Score:5.55|P-value:1.55E-6 ID:SGTC_2414|Compound:st077232|FD-Score:-3.89|P-value:6.69E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-5.12|P-value:1.17E-5 ID:SGTC_2449|Compound:5812982|FD-Score:-6.52|P-value:3.34E-8 ID:SGTC_2479|Compound:5617353|FD-Score:-4.08|P-value:3.79E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-5.09|P-value:1.30E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-5.09|P-value:1.29E-5 ID:SGTC_2488|Compound:5268135|FD-Score:5.77|P-value:6.33E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:6.65|P-value:1.21E-8 ID:SGTC_2570|Compound:licareol|FD-Score:6.43|P-value:3.42E-8 ID:SGTC_2691|Compound:st077356|FD-Score:4.61|P-value:5.34E-5 ID:SGTC_2727|Compound:riluzole|FD-Score:3.85|P-value:5.79E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.88|P-value:6.80E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.12|P-value:2.05E-7 ID:SGTC_2903|Compound:5923464|FD-Score:3.67|P-value:9.77E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.78|P-value:7.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.37|P-value:1.56E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-4.11|P-value:3.44E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.85|P-value:7.42E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-3.86|P-value:7.32E-4

Top fitness defect scores for YCR003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 13.70 5.3 µM 1.08E-30 126 5.04E-43
2 SGTC_1742 st038235 8.72 28.9 µM 1.42E-13 mitochondrial stress 19 1.45E-18
3 SGTC_582 0987-0078 7.85 156.0 µM 2.32E-11 calcium & mitochondrial duress 15 2.04E-15
4 SGTC_1812 st052057 7.01 4.3 µM 2.11E-9 calcium & mitochondrial duress 5 1.23E-12
5 SGTC_2533 agelasine 6.65 13.7 µM 1.21E-8 ERG2 9 1.47E-11
6 SGTC_310 1273-0058 6.52 1.1 µM 2.19E-8 mitochondrial stress 130 3.40E-11
7 SGTC_2570 licareol 6.43 100.0 µM 3.42E-8 2 6.41E-11
8 SGTC_33 0986-0033 6.34 35.8 µM 5.12E-8 calcium & mitochondrial duress 5 1.13E-10
9 SGTC_386 1509-0011 6.05 3.1 µM 1.90E-7 93 7.25E-10
10 SGTC_2232 chlorophetanol 5.88 41.5 µM 3.92E-7 TRP & mitochondrial translation 43 2.02E-9
11 SGTC_2488 5268135 5.77 45.7 µM 6.33E-7 19 3.98E-9
12 SGTC_2250 alverine citrate 5.66 130.0 µM 9.79E-7 64 7.38E-9
13 SGTC_2283 7944793 5.55 200.0 µM 1.55E-6 10 1.42E-8
14 SGTC_173 1273-0061 5.55 2.7 µM 1.60E-6 mitochondrial stress 121 1.47E-8
15 SGTC_39 0986-0283 5.51 58.4 µM 1.85E-6 calcium & mitochondrial duress 10 1.81E-8
16 SGTC_1939 st074455 5.40 53.6 µM 2.88E-6 8 3.38E-8
17 SGTC_449 xestospongin c 5.28 11.2 µM 4.62E-6 NEO1-PIK1 25 6.58E-8
18 SGTC_1627 st003713 5.13 25.6 µM 7.96E-6 calcium & mitochondrial duress 40 1.42E-7
19 SGTC_63 0099-0242 5.11 26.7 µM 8.76E-6 mitochondrial stress 54 1.62E-7
20 SGTC_456 gingerol 4.90 170.0 µM 1.90E-5 7 4.82E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.443 2.54E-160 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.418 1.91E-142 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.401 3.70E-130 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.400 2.75E-129 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.380 1.25E-115 YDR296W MHR1 hom Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.380 1.81E-115 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.371 2.79E-110 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.370 1.91E-109 YMR293C HER2 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
0.365 2.38E-106 YOL095C HMI1 hom Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.365 5.67E-106 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
0.365 5.22E-106 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.364 1.76E-105 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.363 3.52E-105 YDR268W MSW1 hom Mitochondrial tryptophanyl-tRNA synthetase
0.362 2.53E-104 YDR332W IRC3 hom Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.360 3.41E-103 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated