YCR014C / POL4

DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR014C / POL4

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:4.24|P-value:8.00E-6 ID:SGTC_72|Compound:0744-0168|FD-Score:-6.36|P-value:4.50E-11 ID:SGTC_235|Compound:0330-0087|FD-Score:3.08|P-value:8.61E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.37|P-value:3.04E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.64|P-value:1.14E-6 ID:SGTC_1092|Compound:toremifene|FD-Score:3.70|P-value:8.09E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.69|P-value:8.72E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.06|P-value:9.28E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.29|P-value:4.10E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-3.31|P-value:3.73E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.12|P-value:7.57E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.41|P-value:2.56E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.09|P-value:8.32E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.58|P-value:1.33E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.25|P-value:4.65E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.04|P-value:9.67E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.94|P-value:3.05E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.85|P-value:4.44E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.24|P-value:7.80E-6 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.68|P-value:8.83E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.91|P-value:3.43E-5 ID:SGTC_2809|Compound:7964133|FD-Score:-3.16|P-value:6.49E-4 ID:SGTC_3041|Compound:9091929|FD-Score:3.22|P-value:5.18E-4 ID:SGTC_3128|Compound:9127691|FD-Score:4.30|P-value:6.04E-6 ID:SGTC_3137|Compound:9097352|FD-Score:-3.46|P-value:2.13E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.19|P-value:5.81E-4 ID:SGTC_3145|Compound:9085516|FD-Score:5.57|P-value:6.84E-9 ID:SGTC_3202|Compound:9113761|FD-Score:3.55|P-value:1.49E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.62|P-value:1.28E-6 ID:SGTC_3241|Compound:9133421|FD-Score:3.19|P-value:5.75E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:4.24|P-value:8.00E-6 ID:SGTC_72|Compound:0744-0168|FD-Score:-6.36|P-value:4.50E-11 ID:SGTC_235|Compound:0330-0087|FD-Score:3.08|P-value:8.61E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.37|P-value:3.04E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.64|P-value:1.14E-6 ID:SGTC_1092|Compound:toremifene|FD-Score:3.70|P-value:8.09E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.69|P-value:8.72E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.06|P-value:9.28E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.29|P-value:4.10E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-3.31|P-value:3.73E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.12|P-value:7.57E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.41|P-value:2.56E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.09|P-value:8.32E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.58|P-value:1.33E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.25|P-value:4.65E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.04|P-value:9.67E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.94|P-value:3.05E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.85|P-value:4.44E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.24|P-value:7.80E-6 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.68|P-value:8.83E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.91|P-value:3.43E-5 ID:SGTC_2809|Compound:7964133|FD-Score:-3.16|P-value:6.49E-4 ID:SGTC_3041|Compound:9091929|FD-Score:3.22|P-value:5.18E-4 ID:SGTC_3128|Compound:9127691|FD-Score:4.30|P-value:6.04E-6 ID:SGTC_3137|Compound:9097352|FD-Score:-3.46|P-value:2.13E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.19|P-value:5.81E-4 ID:SGTC_3145|Compound:9085516|FD-Score:5.57|P-value:6.84E-9 ID:SGTC_3202|Compound:9113761|FD-Score:3.55|P-value:1.49E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.62|P-value:1.28E-6 ID:SGTC_3241|Compound:9133421|FD-Score:3.19|P-value:5.75E-4

Top fitness defect scores for YCR014C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3145 9085516 5.57 49.5 µM 6.84E-9 fatty acid desaturase (OLE1) 9 1.24E-8
2 SGTC_3210 9128157 4.62 49.5 µM 1.28E-6 30 1.93E-6
3 SGTC_3128 9127691 4.30 49.5 µM 6.04E-6 18 8.67E-6
4 SGTC_68 0986-0284 4.24 82.8 µM 8.00E-6 56 1.14E-5
5 SGTC_2450 5739737 3.94 12.9 µM 3.05E-5 26 4.14E-5
6 SGTC_2639 lactacystin 3.85 53.0 µM 4.44E-5 25 5.95E-5
7 SGTC_1092 toremifene 3.70 6.3 µM 8.09E-5 27 1.06E-4
8 SGTC_1368 1852-0141 3.69 284.0 µM 8.72E-5 ERG2 45 1.14E-4
9 SGTC_2649 anisomycin 3.68 9.6 µM 8.83E-5 12 1.15E-4
10 SGTC_2051 5263410 3.58 176.0 µM 1.33E-4 26 1.72E-4
11 SGTC_3202 9113761 3.55 49.5 µM 1.49E-4 62 1.91E-4
12 SGTC_489 paxilline 3.53 115.0 µM 1.64E-4 PDR1 26 2.10E-4
13 SGTC_1788 fluconazole 3.41 20.0 µM 2.56E-4 azole & statin 80 3.23E-4
14 SGTC_632 0781-2353 3.37 4.7 µM 3.04E-4 Golgi 69 3.81E-4
15 SGTC_1495 4469-0029 3.29 237.0 µM 4.10E-4 158 5.09E-4
16 SGTC_2279 7948423 3.25 32.9 µM 4.65E-4 30 5.75E-4
17 SGTC_3041 9091929 3.22 49.5 µM 5.18E-4 redox potentiating 46 6.38E-4
18 SGTC_3241 9133421 3.19 49.5 µM 5.75E-4 PDR1 6 7.05E-4
19 SGTC_3141 9094609 3.19 49.5 µM 5.81E-4 iron homeostasis 71 7.13E-4
20 SGTC_1759 st043059 3.12 70.0 µM 7.57E-4 amide catabolism 58 9.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 4.87E-24 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.108 4.01E-10 YNL281W HCH1 hom Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.104 1.34E-9 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.099 7.92E-9 YOL015W IRC10 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
0.099 9.02E-9 YKL142W MRP8 hom Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
0.098 1.31E-8 YDR105C TMS1 hom Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
0.083 1.35E-6 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.082 1.88E-6 YOR075W UFE1 het t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
0.082 2.07E-6 YGL257C MNT2 hom Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans
0.080 3.62E-6 YDL079C MRK1 hom Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation
0.080 3.83E-6 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.078 6.90E-6 YJR010W MET3 hom ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism
0.077 7.68E-6 YKL152C GPM1 het Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
0.077 7.88E-6 YHR001W-A QCR10 hom Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
0.076 9.23E-6 YBR205W KTR3 hom Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress