YCR035C / RRP43

Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCR035C / RRP43

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-3.87|P-value:4.77E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.61|P-value:4.47E-5 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.72|P-value:7.48E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.00|P-value:3.42E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-4.16|P-value:1.92E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-4.58|P-value:4.53E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:4.09|P-value:2.60E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.85|P-value:5.36E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.82|P-value:2.11E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.64|P-value:8.26E-9 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.06|P-value:2.59E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-4.35|P-value:1.00E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.78|P-value:6.13E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.71|P-value:3.09E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.52|P-value:1.20E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:-4.26|P-value:1.39E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.19|P-value:5.19E-6 ID:SGTC_490|Compound:phenamil|FD-Score:-5.21|P-value:4.36E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:-4.20|P-value:1.65E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.03|P-value:9.65E-6 ID:SGTC_610|Compound:4215-0184|FD-Score:4.38|P-value:1.01E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:7.22|P-value:4.10E-10 ID:SGTC_879|Compound:0831-1030|FD-Score:3.64|P-value:9.89E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-5.12|P-value:6.08E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.82|P-value:5.48E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:5.92|P-value:2.43E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:-8.11|P-value:2.33E-12 ID:SGTC_1230|Compound:0170-0365|FD-Score:6.28|P-value:4.70E-8 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.01|P-value:3.08E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:7.19|P-value:4.88E-10 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.63|P-value:4.18E-5 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.96|P-value:3.87E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.27|P-value:1.42E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.90|P-value:4.64E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:4.12|P-value:2.36E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.73|P-value:7.13E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-3.76|P-value:6.63E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.07|P-value:7.48E-6 ID:SGTC_1636|Compound:st007707|FD-Score:-4.85|P-value:1.73E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:5.57|P-value:1.08E-6 ID:SGTC_1700|Compound:st030840|FD-Score:5.01|P-value:1.03E-5 ID:SGTC_1707|Compound:st032323|FD-Score:-3.78|P-value:6.27E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.77|P-value:6.31E-4 ID:SGTC_1962|Compound:st076595|FD-Score:4.32|P-value:1.20E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.84|P-value:3.43E-7 ID:SGTC_2087|Compound:5276107|FD-Score:4.73|P-value:2.88E-5 ID:SGTC_2100|Compound:5377183|FD-Score:-4.19|P-value:1.70E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-5.56|P-value:1.04E-6 ID:SGTC_2183|Compound:6420625|FD-Score:4.05|P-value:2.93E-4 ID:SGTC_2290|Compound:7904846|FD-Score:4.86|P-value:1.82E-5 ID:SGTC_2365|Compound:9042475|FD-Score:5.45|P-value:1.78E-6 ID:SGTC_2379|Compound:9035213|FD-Score:-4.14|P-value:2.05E-4 ID:SGTC_2380|Compound:9035439|FD-Score:4.90|P-value:1.55E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-6.67|P-value:6.26E-9 ID:SGTC_2488|Compound:5268135|FD-Score:-4.20|P-value:1.66E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.79|P-value:2.34E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.66|P-value:8.71E-4 ID:SGTC_2831|Compound:7999036|FD-Score:4.40|P-value:9.15E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.86|P-value:5.24E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.93|P-value:3.98E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.58|P-value:5.02E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-5.00|P-value:9.94E-6 ID:SGTC_3019|Compound:9081971|FD-Score:4.18|P-value:1.94E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.55|P-value:5.02E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-4.19|P-value:1.73E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.87|P-value:4.77E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.61|P-value:4.47E-5 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.72|P-value:7.48E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.00|P-value:3.42E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-4.16|P-value:1.92E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-4.58|P-value:4.53E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:4.09|P-value:2.60E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.85|P-value:5.36E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.82|P-value:2.11E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.64|P-value:8.26E-9 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.06|P-value:2.59E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-4.35|P-value:1.00E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.78|P-value:6.13E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.71|P-value:3.09E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.52|P-value:1.20E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:-4.26|P-value:1.39E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.19|P-value:5.19E-6 ID:SGTC_490|Compound:phenamil|FD-Score:-5.21|P-value:4.36E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:-4.20|P-value:1.65E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.03|P-value:9.65E-6 ID:SGTC_610|Compound:4215-0184|FD-Score:4.38|P-value:1.01E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:7.22|P-value:4.10E-10 ID:SGTC_879|Compound:0831-1030|FD-Score:3.64|P-value:9.89E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-5.12|P-value:6.08E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.82|P-value:5.48E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:5.92|P-value:2.43E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:-8.11|P-value:2.33E-12 ID:SGTC_1230|Compound:0170-0365|FD-Score:6.28|P-value:4.70E-8 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.01|P-value:3.08E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:7.19|P-value:4.88E-10 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.63|P-value:4.18E-5 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.96|P-value:3.87E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.27|P-value:1.42E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.90|P-value:4.64E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:4.12|P-value:2.36E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.73|P-value:7.13E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-3.76|P-value:6.63E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.07|P-value:7.48E-6 ID:SGTC_1636|Compound:st007707|FD-Score:-4.85|P-value:1.73E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:5.57|P-value:1.08E-6 ID:SGTC_1700|Compound:st030840|FD-Score:5.01|P-value:1.03E-5 ID:SGTC_1707|Compound:st032323|FD-Score:-3.78|P-value:6.27E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.77|P-value:6.31E-4 ID:SGTC_1962|Compound:st076595|FD-Score:4.32|P-value:1.20E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.84|P-value:3.43E-7 ID:SGTC_2087|Compound:5276107|FD-Score:4.73|P-value:2.88E-5 ID:SGTC_2100|Compound:5377183|FD-Score:-4.19|P-value:1.70E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-5.56|P-value:1.04E-6 ID:SGTC_2183|Compound:6420625|FD-Score:4.05|P-value:2.93E-4 ID:SGTC_2290|Compound:7904846|FD-Score:4.86|P-value:1.82E-5 ID:SGTC_2365|Compound:9042475|FD-Score:5.45|P-value:1.78E-6 ID:SGTC_2379|Compound:9035213|FD-Score:-4.14|P-value:2.05E-4 ID:SGTC_2380|Compound:9035439|FD-Score:4.90|P-value:1.55E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-6.67|P-value:6.26E-9 ID:SGTC_2488|Compound:5268135|FD-Score:-4.20|P-value:1.66E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.79|P-value:2.34E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.66|P-value:8.71E-4 ID:SGTC_2831|Compound:7999036|FD-Score:4.40|P-value:9.15E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.86|P-value:5.24E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.93|P-value:3.98E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.58|P-value:5.02E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-5.00|P-value:9.94E-6 ID:SGTC_3019|Compound:9081971|FD-Score:4.18|P-value:1.94E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.55|P-value:5.02E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-4.19|P-value:1.73E-4

Top fitness defect scores for YCR035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_617 3770-0098 7.22 35.0 µM 4.10E-10 exosome 12 2.58E-13 0.54
2 SGTC_1265 0819-1001 7.19 68.8 µM 4.88E-10 1 3.27E-13 1.46
3 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 6.64 4.6 µM 8.26E-9 heat shock/prefoldin 8 1.58E-11 0.85
4 SGTC_1230 0170-0365 6.28 3.8 µM 4.70E-8 endomembrane recycling 10 1.71E-10 0.06
5 SGTC_1127 asiatic acid 5.92 681.4 nM 2.43E-7 2 1.62E-9 0.66
6 SGTC_2021 4019055 5.84 173.0 µM 3.43E-7 endomembrane recycling 19 2.60E-9 0.16
7 SGTC_1698 5-fluorocytosine 5.57 1.1 µM 1.08E-6 exosome 12 1.25E-8 1.67
8 SGTC_2365 9042475 5.45 200.0 µM 1.78E-6 3 2.47E-8 0.72
9 SGTC_457 grayanotoxin iii 5.19 135.0 µM 5.19E-6 13 1.06E-7 0.13
10 SGTC_524 r073-0010 5.03 27.8 µM 9.65E-6 7 2.48E-7 0.09
11 SGTC_1700 st030840 5.01 7.9 µM 1.03E-5 PDR1 1 2.71E-7 0.65
12 SGTC_2380 9035439 4.90 200.0 µM 1.55E-5 endomembrane recycling 4 4.71E-7 0.12
13 SGTC_2290 7904846 4.86 200.0 µM 1.82E-5 8 5.88E-7 0.38
14 SGTC_221 trifluoperazine 4.82 10.3 µM 2.11E-5 NEO1-PIK1 22 7.21E-7 0.03
15 SGTC_2714 st079313 4.79 56.9 µM 2.34E-5 2 8.30E-7 0.12
16 SGTC_2087 5276107 4.73 200.0 µM 2.88E-5 2 1.10E-6 0.27
17 SGTC_451 chloral hydrate 4.71 100.0 µM 3.09E-5 9 1.21E-6 0.03
18 SGTC_1308 1187-1617 4.63 41.4 µM 4.18E-5 fatty acid desaturase (OLE1) 5 1.83E-6 0.04
19 SGTC_70 0141-0289 4.61 43.3 µM 4.47E-5 mitochondrial stress 16 2.00E-6 0.58
20 SGTC_2993 9054257 4.58 71.4 µM 5.02E-5 fatty acid desaturase (OLE1) 25 2.35E-6 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.338 4.40E-90 YOL144W NOP8 het Nucleolar protein required for 60S ribosomal subunit biogenesis
0.295 4.44E-68 YJL086C_d YJL086C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
0.177 4.47E-25 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.171 1.63E-23 YDR317W HIM1 hom Protein of unknown function involved in DNA repair
0.167 1.75E-22 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.157 5.48E-20 YCR052W RSC6 het Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
0.151 1.52E-18 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.150 2.59E-18 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.149 5.08E-18 YOR206W NOC2 het Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
0.142 1.27E-16 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.133 1.16E-14 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.129 5.63E-14 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.123 9.71E-13 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.120 2.79E-12 YHR069C RRP4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
0.119 5.45E-12 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p