YCR047C / BUD23

Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR047C / BUD23

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:5.72|P-value:8.15E-9 ID:SGTC_232|Compound:clozapine|FD-Score:3.13|P-value:9.06E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.75|P-value:1.64E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:3.63|P-value:1.56E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:6.01|P-value:1.58E-9 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.10|P-value:4.08E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.25|P-value:9.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:7.40E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.25|P-value:1.26E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.51|P-value:2.46E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.26|P-value:1.24E-5 ID:SGTC_702|Compound:1488-0190|FD-Score:3.20|P-value:7.35E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.36|P-value:4.23E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:7.29|P-value:3.65E-13 ID:SGTC_722|Compound:3534-0461|FD-Score:3.62|P-value:1.61E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.83|P-value:7.25E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.70|P-value:1.19E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.72|P-value:1.10E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:5.11|P-value:2.27E-7 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.10|P-value:2.39E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.15|P-value:8.59E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.43|P-value:3.24E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-8.04|P-value:3.59E-15 ID:SGTC_1718|Compound:roemerine|FD-Score:-10.00|P-value:1.19E-22 ID:SGTC_1720|Compound:st037298|FD-Score:3.55|P-value:2.12E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.92|P-value:8.38E-5 ID:SGTC_1769|Compound:st044406|FD-Score:4.24|P-value:1.35E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.54|P-value:2.19E-4 ID:SGTC_1921|Compound:5740136|FD-Score:4.08|P-value:2.61E-5 ID:SGTC_2213|Compound:7173844|FD-Score:3.21|P-value:7.06E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.29|P-value:5.40E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.60|P-value:2.77E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.11|P-value:2.32E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.10|P-value:0.00100 ID:SGTC_2335|Compound:9018011|FD-Score:3.28|P-value:5.58E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.06|P-value:2.85E-5 ID:SGTC_2379|Compound:9035213|FD-Score:3.66|P-value:1.41E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.43|P-value:4.15E-8 ID:SGTC_2434|Compound:5670830|FD-Score:5.68|P-value:1.08E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.34|P-value:4.50E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.11|P-value:9.95E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:3.12|P-value:9.45E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.28|P-value:8.51E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.25|P-value:9.50E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.17|P-value:1.80E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-5.07|P-value:5.23E-7 ID:SGTC_2889|Compound:9059656|FD-Score:4.11|P-value:2.32E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-6.85|P-value:1.84E-11 ID:SGTC_2908|Compound:7930301|FD-Score:3.37|P-value:4.10E-4 ID:SGTC_3043|Compound:9092303|FD-Score:3.46|P-value:2.94E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.84|P-value:6.86E-5 ID:SGTC_3136|Compound:9095672|FD-Score:4.17|P-value:1.78E-5 ID:SGTC_3141|Compound:9094609|FD-Score:4.71|P-value:1.56E-6 ID:SGTC_3160|Compound:9100216|FD-Score:-3.32|P-value:7.32E-4 ID:SGTC_3175|Compound:9106517|FD-Score:4.36|P-value:7.86E-6 ID:SGTC_3188|Compound:9110789|FD-Score:-3.54|P-value:3.42E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.57|P-value:3.03E-6 ID:SGTC_3235|Compound:9132602|FD-Score:4.19|P-value:1.63E-5 ID:SGTC_3236|Compound:9132701|FD-Score:-3.78|P-value:1.43E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.35|P-value:4.28E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.22|P-value:6.79E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-4.87|P-value:1.39E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:5.72|P-value:8.15E-9 ID:SGTC_232|Compound:clozapine|FD-Score:3.13|P-value:9.06E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.75|P-value:1.64E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:3.63|P-value:1.56E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:6.01|P-value:1.58E-9 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.10|P-value:4.08E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.25|P-value:9.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:7.40E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.25|P-value:1.26E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.51|P-value:2.46E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.26|P-value:1.24E-5 ID:SGTC_702|Compound:1488-0190|FD-Score:3.20|P-value:7.35E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.36|P-value:4.23E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:7.29|P-value:3.65E-13 ID:SGTC_722|Compound:3534-0461|FD-Score:3.62|P-value:1.61E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.83|P-value:7.25E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.70|P-value:1.19E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.72|P-value:1.10E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:5.11|P-value:2.27E-7 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.10|P-value:2.39E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.15|P-value:8.59E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.43|P-value:3.24E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-8.04|P-value:3.59E-15 ID:SGTC_1718|Compound:roemerine|FD-Score:-10.00|P-value:1.19E-22 ID:SGTC_1720|Compound:st037298|FD-Score:3.55|P-value:2.12E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.92|P-value:8.38E-5 ID:SGTC_1769|Compound:st044406|FD-Score:4.24|P-value:1.35E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.54|P-value:2.19E-4 ID:SGTC_1921|Compound:5740136|FD-Score:4.08|P-value:2.61E-5 ID:SGTC_2213|Compound:7173844|FD-Score:3.21|P-value:7.06E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.29|P-value:5.40E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.60|P-value:2.77E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.11|P-value:2.32E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.10|P-value:0.00100 ID:SGTC_2335|Compound:9018011|FD-Score:3.28|P-value:5.58E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.06|P-value:2.85E-5 ID:SGTC_2379|Compound:9035213|FD-Score:3.66|P-value:1.41E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.43|P-value:4.15E-8 ID:SGTC_2434|Compound:5670830|FD-Score:5.68|P-value:1.08E-8 ID:SGTC_2437|Compound:5741531|FD-Score:3.34|P-value:4.50E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.11|P-value:9.95E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:3.12|P-value:9.45E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.28|P-value:8.51E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.25|P-value:9.50E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.17|P-value:1.80E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-5.07|P-value:5.23E-7 ID:SGTC_2889|Compound:9059656|FD-Score:4.11|P-value:2.32E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-6.85|P-value:1.84E-11 ID:SGTC_2908|Compound:7930301|FD-Score:3.37|P-value:4.10E-4 ID:SGTC_3043|Compound:9092303|FD-Score:3.46|P-value:2.94E-4 ID:SGTC_3069|Compound:9117009|FD-Score:3.84|P-value:6.86E-5 ID:SGTC_3136|Compound:9095672|FD-Score:4.17|P-value:1.78E-5 ID:SGTC_3141|Compound:9094609|FD-Score:4.71|P-value:1.56E-6 ID:SGTC_3160|Compound:9100216|FD-Score:-3.32|P-value:7.32E-4 ID:SGTC_3175|Compound:9106517|FD-Score:4.36|P-value:7.86E-6 ID:SGTC_3188|Compound:9110789|FD-Score:-3.54|P-value:3.42E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.57|P-value:3.03E-6 ID:SGTC_3235|Compound:9132602|FD-Score:4.19|P-value:1.63E-5 ID:SGTC_3236|Compound:9132701|FD-Score:-3.78|P-value:1.43E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.35|P-value:4.28E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.22|P-value:6.79E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-4.87|P-value:1.39E-6

Top fitness defect scores for YCR047C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_710 3448-4187 7.29 58.0 µM 3.65E-13 fatty acid desaturase (OLE1) 9 1.56E-13
2 SGTC_343 4130-2827 6.01 29.8 µM 1.58E-9 5 9.43E-10
3 SGTC_118 0083-0097 5.72 135.3 µM 8.15E-9 translation 33 5.18E-9
4 SGTC_2434 5670830 5.68 169.9 µM 1.08E-8 5 6.92E-9
5 SGTC_2422 chlorphenesin carbamate 5.43 384.2 µM 4.15E-8 8 2.81E-8
6 SGTC_1281 0884-0031 5.11 11.8 µM 2.27E-7 fatty acid desaturase (OLE1) 10 1.64E-7
7 SGTC_3141 9094609 4.71 49.5 µM 1.56E-6 iron homeostasis 22 1.21E-6
8 SGTC_3217 9129673 4.57 49.5 µM 3.03E-6 19 2.40E-6
9 SGTC_3175 9106517 4.36 49.5 µM 7.86E-6 iron homeostasis 14 6.43E-6
10 SGTC_625 k213-0058 4.26 121.0 µM 1.24E-5 translation 83 1.03E-5
11 SGTC_552 k242-0005 4.25 476.0 µM 1.26E-5 11 1.05E-5
12 SGTC_1769 st044406 4.24 64.4 µM 1.35E-5 8 1.13E-5
13 SGTC_3235 9132602 4.19 49.5 µM 1.63E-5 18 1.37E-5
14 SGTC_3136 9095672 4.17 49.5 µM 1.78E-5 20 1.50E-5
15 SGTC_2881 9058953 4.17 52.0 µM 1.80E-5 14 1.52E-5
16 SGTC_2889 9059656 4.11 52.0 µM 2.32E-5 7 1.97E-5
17 SGTC_2325 6158266 4.11 200.0 µM 2.32E-5 17 1.97E-5
18 SGTC_1328 1441-0025 4.10 69.2 µM 2.39E-5 6 2.03E-5
19 SGTC_1921 5740136 4.08 50.0 µM 2.61E-5 TRP & mitochondrial translation 50 2.22E-5
20 SGTC_2376 9072922 4.06 81.3 µM 2.85E-5 26 2.43E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.197 1.21E-30 YGL135W RPL1B hom Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.171 2.11E-23 YDR161W_p YDR161W_p hom Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
0.142 1.59E-16 YJL003W COX16 hom Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
0.141 1.97E-16 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.140 4.16E-16 YBR017C KAP104 hom Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis
0.135 4.10E-15 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.133 1.30E-14 YPL118W MRP51 hom Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
0.132 2.06E-14 YOR170W_d YOR170W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4
0.128 1.02E-13 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.125 3.78E-13 YDR296W MHR1 hom Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.122 1.28E-12 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.119 3.81E-12 YLR448W RPL6B hom Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
0.118 6.38E-12 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.118 7.70E-12 YNL284C MRPL10 hom Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
0.118 8.43E-12 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay