YCR053W / THR4

Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR053W / THR4

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:28.50|P-value:3.27E-221 ID:SGTC_18|Compound:0111-0177|FD-Score:6.77|P-value:2.39E-14 ID:SGTC_59|Compound:0330-0160|FD-Score:25.20|P-value:6.56E-173 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.20|P-value:1.88E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.68|P-value:1.23E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:3.26|P-value:1.37E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.82|P-value:3.99E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:29.00|P-value:7.48E-229 ID:SGTC_177|Compound:1327-0068|FD-Score:8.81|P-value:5.30E-23 ID:SGTC_195|Compound:4234-0266|FD-Score:3.31|P-value:1.11E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:-3.00|P-value:4.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:31.80|P-value:1.55E-274 ID:SGTC_264|Compound:promethazine|FD-Score:-3.07|P-value:3.20E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.32|P-value:1.07E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.84|P-value:7.83E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:2.87|P-value:6.82E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:16.30|P-value:5.93E-74 ID:SGTC_371|Compound:0195-0006|FD-Score:3.86|P-value:8.63E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:18.50|P-value:1.54E-94 ID:SGTC_513|Compound:4245-1575|FD-Score:5.99|P-value:1.23E-11 ID:SGTC_551|Compound:k048-0028|FD-Score:3.08|P-value:2.93E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.89|P-value:7.31E-6 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.26|P-value:1.44E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:4.07|P-value:2.85E-6 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.07|P-value:3.27E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:26.10|P-value:3.28E-185 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.24|P-value:1.61E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.21|P-value:1.79E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.96|P-value:5.00E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.13|P-value:2.57E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.04|P-value:3.70E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.47|P-value:5.86E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:4.17|P-value:1.69E-6 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.05|P-value:3.38E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:3.83|P-value:9.90E-6 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.94|P-value:1.82E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.64|P-value:1.27E-7 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.46|P-value:6.45E-10 ID:SGTC_1635|Compound:st007035|FD-Score:14.70|P-value:4.15E-60 ID:SGTC_1740|Compound:st038286|FD-Score:-3.45|P-value:6.39E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:12.50|P-value:1.75E-44 ID:SGTC_1900|Compound:st060200|FD-Score:-3.09|P-value:3.00E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.56|P-value:3.72E-5 ID:SGTC_2014|Compound:4013930|FD-Score:3.86|P-value:8.53E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-4.33|P-value:7.23E-7 ID:SGTC_2017|Compound:5132927|FD-Score:-4.54|P-value:2.29E-7 ID:SGTC_2018|Compound:5133068|FD-Score:3.33|P-value:1.04E-4 ID:SGTC_2048|Compound:5246248|FD-Score:2.98|P-value:4.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.07|P-value:3.11E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.21|P-value:3.07E-20 ID:SGTC_2318|Compound:6029776|FD-Score:3.85|P-value:8.79E-6 ID:SGTC_2332|Compound:7982454|FD-Score:-3.09|P-value:2.97E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.00|P-value:4.18E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.31|P-value:1.16E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.49|P-value:5.10E-5 ID:SGTC_2430|Compound:5571206|FD-Score:9.45|P-value:3.42E-26 ID:SGTC_2456|Compound:5770359|FD-Score:2.85|P-value:7.44E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.14|P-value:2.41E-4 ID:SGTC_2487|Compound:5918373|FD-Score:2.82|P-value:8.41E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-2.96|P-value:5.09E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.40|P-value:7.54E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:21.80|P-value:2.88E-130 ID:SGTC_2679|Compound:rapamycin|FD-Score:25.60|P-value:4.50E-179 ID:SGTC_2706|Compound:st078239|FD-Score:-3.27|P-value:1.39E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.82|P-value:1.06E-5 ID:SGTC_2831|Compound:7999036|FD-Score:-3.04|P-value:3.72E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.10|P-value:2.85E-4 ID:SGTC_2930|Compound:9013230|FD-Score:2.95|P-value:4.98E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:14.10|P-value:7.60E-56 ID:SGTC_3060|Compound:1327-0027|FD-Score:8.44|P-value:2.73E-21 ID:SGTC_3063|Compound:1327-0034|FD-Score:10.40|P-value:1.91E-31 ID:SGTC_3064|Compound:1327-0027|FD-Score:3.74|P-value:1.58E-5 ID:SGTC_3078|Compound:9116413|FD-Score:-3.22|P-value:1.71E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.55|P-value:3.84E-5 ID:SGTC_3185|Compound:9108267|FD-Score:-2.87|P-value:7.16E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.86|P-value:7.28E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.10|P-value:2.49E-6 ID:SGTC_3308|Compound:9128332|FD-Score:3.06|P-value:3.26E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.13|P-value:2.48E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-2.95|P-value:5.21E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:28.50|P-value:3.27E-221 ID:SGTC_18|Compound:0111-0177|FD-Score:6.77|P-value:2.39E-14 ID:SGTC_59|Compound:0330-0160|FD-Score:25.20|P-value:6.56E-173 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.20|P-value:1.88E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.68|P-value:1.23E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:3.26|P-value:1.37E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.82|P-value:3.99E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:29.00|P-value:7.48E-229 ID:SGTC_177|Compound:1327-0068|FD-Score:8.81|P-value:5.30E-23 ID:SGTC_195|Compound:4234-0266|FD-Score:3.31|P-value:1.11E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:-3.00|P-value:4.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:31.80|P-value:1.55E-274 ID:SGTC_264|Compound:promethazine|FD-Score:-3.07|P-value:3.20E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.32|P-value:1.07E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.84|P-value:7.83E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:2.87|P-value:6.82E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:16.30|P-value:5.93E-74 ID:SGTC_371|Compound:0195-0006|FD-Score:3.86|P-value:8.63E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:18.50|P-value:1.54E-94 ID:SGTC_513|Compound:4245-1575|FD-Score:5.99|P-value:1.23E-11 ID:SGTC_551|Compound:k048-0028|FD-Score:3.08|P-value:2.93E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.89|P-value:7.31E-6 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.26|P-value:1.44E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:4.07|P-value:2.85E-6 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.07|P-value:3.27E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:26.10|P-value:3.28E-185 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.24|P-value:1.61E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.21|P-value:1.79E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.96|P-value:5.00E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.13|P-value:2.57E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.04|P-value:3.70E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.47|P-value:5.86E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:4.17|P-value:1.69E-6 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.05|P-value:3.38E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:3.83|P-value:9.90E-6 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.94|P-value:1.82E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.64|P-value:1.27E-7 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.46|P-value:6.45E-10 ID:SGTC_1635|Compound:st007035|FD-Score:14.70|P-value:4.15E-60 ID:SGTC_1740|Compound:st038286|FD-Score:-3.45|P-value:6.39E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:12.50|P-value:1.75E-44 ID:SGTC_1900|Compound:st060200|FD-Score:-3.09|P-value:3.00E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.56|P-value:3.72E-5 ID:SGTC_2014|Compound:4013930|FD-Score:3.86|P-value:8.53E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-4.33|P-value:7.23E-7 ID:SGTC_2017|Compound:5132927|FD-Score:-4.54|P-value:2.29E-7 ID:SGTC_2018|Compound:5133068|FD-Score:3.33|P-value:1.04E-4 ID:SGTC_2048|Compound:5246248|FD-Score:2.98|P-value:4.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.07|P-value:3.11E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.21|P-value:3.07E-20 ID:SGTC_2318|Compound:6029776|FD-Score:3.85|P-value:8.79E-6 ID:SGTC_2332|Compound:7982454|FD-Score:-3.09|P-value:2.97E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.00|P-value:4.18E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.31|P-value:1.16E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.49|P-value:5.10E-5 ID:SGTC_2430|Compound:5571206|FD-Score:9.45|P-value:3.42E-26 ID:SGTC_2456|Compound:5770359|FD-Score:2.85|P-value:7.44E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.14|P-value:2.41E-4 ID:SGTC_2487|Compound:5918373|FD-Score:2.82|P-value:8.41E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-2.96|P-value:5.09E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.40|P-value:7.54E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:21.80|P-value:2.88E-130 ID:SGTC_2679|Compound:rapamycin|FD-Score:25.60|P-value:4.50E-179 ID:SGTC_2706|Compound:st078239|FD-Score:-3.27|P-value:1.39E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.82|P-value:1.06E-5 ID:SGTC_2831|Compound:7999036|FD-Score:-3.04|P-value:3.72E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.10|P-value:2.85E-4 ID:SGTC_2930|Compound:9013230|FD-Score:2.95|P-value:4.98E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:14.10|P-value:7.60E-56 ID:SGTC_3060|Compound:1327-0027|FD-Score:8.44|P-value:2.73E-21 ID:SGTC_3063|Compound:1327-0034|FD-Score:10.40|P-value:1.91E-31 ID:SGTC_3064|Compound:1327-0027|FD-Score:3.74|P-value:1.58E-5 ID:SGTC_3078|Compound:9116413|FD-Score:-3.22|P-value:1.71E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.55|P-value:3.84E-5 ID:SGTC_3185|Compound:9108267|FD-Score:-2.87|P-value:7.16E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.86|P-value:7.28E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.10|P-value:2.49E-6 ID:SGTC_3308|Compound:9128332|FD-Score:3.06|P-value:3.26E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.13|P-value:2.48E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-2.95|P-value:5.21E-4

Top fitness defect scores for YCR053W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 31.80 5.3 µM 1.55E-274 10 5.01E-222
2 SGTC_163 1327-0036 29.00 7.5 µM 7.48E-229 3 3.80E-185
3 SGTC_7 0192-1582 28.50 4.2 µM 3.27E-221 2 5.61E-179
4 SGTC_852 0418-0143 26.10 439.0 µM 3.28E-185 calcium & mitochondrial duress 1 6.51E-150
5 SGTC_2679 rapamycin 25.60 1.0 nM 4.50E-179 1 5.86E-145
6 SGTC_59 0330-0160 25.20 1.3 µM 6.56E-173 2 5.55E-140
7 SGTC_2651 1327-0036 21.80 5.0 µM 2.88E-130 1 1.49E-105
8 SGTC_386 1509-0011 18.50 3.1 µM 1.54E-94 10 1.05E-76
9 SGTC_310 1273-0058 16.30 1.1 µM 5.93E-74 mitochondrial stress 4 4.42E-60
10 SGTC_1635 st007035 14.70 42.4 µM 4.15E-60 42 6.72E-49
11 SGTC_3059 1327-0034 14.10 3.0 µM 7.60E-56 6 1.87E-45
12 SGTC_1844 rapamycin 12.50 4.0 nM 1.75E-44 19 2.82E-36
13 SGTC_3063 1327-0034 10.40 4.0 µM 1.91E-31 7 9.67E-26
14 SGTC_2430 5571206 9.45 200.0 µM 3.42E-26 9 1.71E-21
15 SGTC_177 1327-0068 8.81 8.4 µM 5.30E-23 11 6.49E-19
16 SGTC_3060 1327-0027 8.44 12.5 µM 2.73E-21 10 1.57E-17
17 SGTC_2233 sulbentine 8.21 40.4 µM 3.07E-20 12 1.12E-16
18 SGTC_18 0111-0177 6.77 500.0 nM 2.39E-14 12 6.53E-12
19 SGTC_513 4245-1575 5.99 41.7 µM 1.23E-11 unfolded protein response 46 1.03E-9
20 SGTC_64 000l-0018 5.68 272.1 µM 1.23E-10 ubiquinone biosynthesis & proteasome 87 6.66E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.690 0 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.503 1.12E-214 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.494 3.34E-205 YDR158W HOM2 hom Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.436 1.63E-155 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.422 1.07E-144 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.420 3.40E-143 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.418 1.30E-141 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.412 1.16E-137 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.406 3.30E-133 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.404 1.06E-131 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.402 2.79E-130 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.402 4.46E-130 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.397 8.96E-127 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.388 1.98E-120 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.386 3.66E-119 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases