Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YCR057C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.163 | 2.16E-21 | YEL032W | MCM3 | het | Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex |
0.152 | 7.78E-19 | YGL002W | ERP6 | hom | Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication |
0.144 | 4.97E-17 | YGL075C | MPS2 | het | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p |
0.125 | 4.35E-13 | YMR168C | CEP3 | het | Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain |
0.123 | 1.04E-12 | YGL200C | EMP24 | hom | Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles |
0.119 | 4.21E-12 | YMR262W_p | YMR262W_p | hom | Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene |
0.119 | 5.41E-12 | YLR083C | EMP70 | hom | Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments |
0.115 | 2.84E-11 | YML047W-A_d | YML047W-A_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.114 | 4.00E-11 | YML018C | YML018C | hom | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication |
0.108 | 3.18E-10 | YPL157W | TGS1 | hom | Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs |
0.108 | 3.32E-10 | YEL064C | AVT2 | hom | Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters |
0.107 | 5.80E-10 | YPL197C_d | YPL197C_d | hom | Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B |
0.103 | 2.40E-9 | YBR056W | YBR056W | hom | Putative glycoside hydrolase of the mitochondrial intermembrane space |
0.103 | 2.63E-9 | YGL118C_d | YGL118C_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.100 | 5.73E-9 | YJL085W | EXO70 | het | Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress |