YCR057C / PWP2

Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCR057C / PWP2

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:4.01|P-value:1.36E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.81|P-value:2.71E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:4.37|P-value:3.52E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.37|P-value:4.84E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:4.94|P-value:3.40E-6 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.70|P-value:3.94E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-4.01|P-value:8.48E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:4.57|P-value:1.61E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.86|P-value:1.44E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-5.60|P-value:8.56E-8 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.76|P-value:3.23E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.55|P-value:6.39E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.47|P-value:5.56E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.63|P-value:5.01E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.73|P-value:8.16E-6 ID:SGTC_1591|Compound:genipin|FD-Score:4.74|P-value:7.98E-6 ID:SGTC_1626|Compound:st003712|FD-Score:6.12|P-value:1.14E-8 ID:SGTC_1697|Compound:st025554|FD-Score:3.65|P-value:4.63E-4 ID:SGTC_1743|Compound:st037606|FD-Score:-3.33|P-value:8.65E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.21|P-value:6.60E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-4.67|P-value:6.08E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.00|P-value:2.15E-8 ID:SGTC_2097|Compound:5376186|FD-Score:4.08|P-value:1.04E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.29|P-value:9.75E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.04|P-value:1.20E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.47|P-value:1.44E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.35|P-value:3.76E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.61|P-value:5.26E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-8.21|P-value:1.11E-14 ID:SGTC_2882|Compound:9058044|FD-Score:4.41|P-value:2.98E-5 ID:SGTC_2899|Compound:9053889|FD-Score:-3.35|P-value:7.97E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-10.30|P-value:5.25E-22 ID:SGTC_2994|Compound:9053520|FD-Score:-6.13|P-value:5.28E-9 ID:SGTC_2997|Compound:9067075|FD-Score:-3.53|P-value:4.51E-4 ID:SGTC_3045|Compound:9092950|FD-Score:-3.30|P-value:9.55E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.51|P-value:4.83E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.37|P-value:7.56E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.62|P-value:3.30E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.94|P-value:1.74E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.63|P-value:4.99E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:4.01|P-value:1.36E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.81|P-value:2.71E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:4.37|P-value:3.52E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.37|P-value:4.84E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:4.94|P-value:3.40E-6 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.70|P-value:3.94E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-4.01|P-value:8.48E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:4.57|P-value:1.61E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.86|P-value:1.44E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-5.60|P-value:8.56E-8 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.76|P-value:3.23E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.55|P-value:6.39E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.47|P-value:5.56E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.63|P-value:5.01E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.73|P-value:8.16E-6 ID:SGTC_1591|Compound:genipin|FD-Score:4.74|P-value:7.98E-6 ID:SGTC_1626|Compound:st003712|FD-Score:6.12|P-value:1.14E-8 ID:SGTC_1697|Compound:st025554|FD-Score:3.65|P-value:4.63E-4 ID:SGTC_1743|Compound:st037606|FD-Score:-3.33|P-value:8.65E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.21|P-value:6.60E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-4.67|P-value:6.08E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.00|P-value:2.15E-8 ID:SGTC_2097|Compound:5376186|FD-Score:4.08|P-value:1.04E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.29|P-value:9.75E-4 ID:SGTC_2467|Compound:5474540|FD-Score:4.04|P-value:1.20E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.47|P-value:1.44E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.35|P-value:3.76E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.61|P-value:5.26E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-8.21|P-value:1.11E-14 ID:SGTC_2882|Compound:9058044|FD-Score:4.41|P-value:2.98E-5 ID:SGTC_2899|Compound:9053889|FD-Score:-3.35|P-value:7.97E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-10.30|P-value:5.25E-22 ID:SGTC_2994|Compound:9053520|FD-Score:-6.13|P-value:5.28E-9 ID:SGTC_2997|Compound:9067075|FD-Score:-3.53|P-value:4.51E-4 ID:SGTC_3045|Compound:9092950|FD-Score:-3.30|P-value:9.55E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.51|P-value:4.83E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.37|P-value:7.56E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.62|P-value:3.30E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.94|P-value:1.74E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.63|P-value:4.99E-4

Top fitness defect scores for YCR057C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1626 st003712 6.12 1.8 µM 1.14E-8 2 4.67E-10 1.46
2 SGTC_1926 methyl fluorone black 6.00 53.6 µM 2.15E-8 DNA intercalators 9 9.89E-10 0.02
3 SGTC_145 3737-0113 5.37 38.4 µM 4.84E-7 DNA intercalators 25 3.91E-8 0.09
4 SGTC_356 1634-0012 4.94 32.5 µM 3.40E-6 4 3.91E-7 0.40
5 SGTC_1591 genipin 4.74 88.4 µM 7.98E-6 mitochondrial processes 2 1.07E-6 0.29
6 SGTC_1545 lovastatin 4.73 49.4 µM 8.16E-6 4 1.10E-6 0.57
7 SGTC_617 3770-0098 4.57 35.0 µM 1.61E-5 exosome 21 2.45E-6 0.02
8 SGTC_2882 9058044 4.41 58.4 µM 2.98E-5 1 5.07E-6 0.21
9 SGTC_126 0417-1624 4.37 672.5 µM 3.52E-5 DNA intercalators 11 6.18E-6 0.04
10 SGTC_2603 homopterocarpin 4.35 100.0 µM 3.76E-5 4 6.68E-6 0.04
11 SGTC_1860 st056228 4.21 74.6 µM 6.60E-5 5 1.30E-5 0.35
12 SGTC_2097 5376186 4.08 200.0 µM 1.04E-4 3 2.22E-5 0.40
13 SGTC_2467 5474540 4.04 200.0 µM 1.20E-4 5 2.62E-5 0.01
14 SGTC_9 0092-0002 4.01 10.4 µM 1.36E-4 13 3.04E-5 0.08
15 SGTC_3206 9114350 3.94 49.5 µM 1.74E-4 3 4.06E-5 0.30
16 SGTC_94 0334-0113 3.81 514.8 µM 2.71E-4 4 6.87E-5 0.54
17 SGTC_1140 3229-1848 3.76 3.0 µM 3.23E-4 6 8.47E-5 0.02
18 SGTC_415 dibenzyl ketone 3.70 100.0 µM 3.94E-4 4 1.07E-4 0.06
19 SGTC_1697 st025554 3.65 27.7 µM 4.63E-4 5 1.30E-4 0.38
20 SGTC_3259 9138485 3.63 49.5 µM 4.99E-4 6 1.41E-4 0.24

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.163 2.16E-21 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.152 7.78E-19 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.144 4.97E-17 YGL075C MPS2 het Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
0.125 4.35E-13 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.123 1.04E-12 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.119 4.21E-12 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.119 5.41E-12 YLR083C EMP70 hom Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments
0.115 2.84E-11 YML047W-A_d YML047W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.114 4.00E-11 YML018C YML018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
0.108 3.18E-10 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.108 3.32E-10 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.107 5.80E-10 YPL197C_d YPL197C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.103 2.40E-9 YBR056W YBR056W hom Putative glycoside hydrolase of the mitochondrial intermembrane space
0.103 2.63E-9 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.100 5.73E-9 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress