YCR063W / BUD31

Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR063W / BUD31

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.10|P-value:5.02E-8 ID:SGTC_117|Compound:lucigenin|FD-Score:6.85|P-value:5.29E-13 ID:SGTC_118|Compound:0083-0097|FD-Score:3.74|P-value:4.33E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.17|P-value:6.91E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.28|P-value:3.85E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.00|P-value:2.48E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:4.27|P-value:4.06E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:7.59|P-value:1.56E-15 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:7.87|P-value:1.44E-16 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:9.38|P-value:1.14E-22 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.73|P-value:4.65E-16 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.94|P-value:2.60E-13 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:9.28|P-value:2.99E-22 ID:SGTC_309|Compound:k408-0051|FD-Score:3.93|P-value:1.91E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.56|P-value:9.35E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.19|P-value:3.93E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:6.15|P-value:7.58E-11 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.24|P-value:5.28E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:5.20|P-value:2.93E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.90|P-value:3.66E-13 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.89|P-value:2.33E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:5.48|P-value:5.65E-9 ID:SGTC_697|Compound:0508-1955|FD-Score:4.66|P-value:5.56E-7 ID:SGTC_715|Compound:0868-0130|FD-Score:5.07|P-value:6.18E-8 ID:SGTC_720|Compound:r052-1644|FD-Score:3.91|P-value:2.11E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:3.55|P-value:9.58E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:4.07|P-value:9.97E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:2.97|P-value:8.88E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.34|P-value:3.65E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.29|P-value:2.76E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.16|P-value:4.50E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.03|P-value:7.16E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.50|P-value:1.21E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.61|P-value:2.44E-9 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.22|P-value:3.51E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.34|P-value:3.69E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.11|P-value:8.48E-6 ID:SGTC_1722|Compound:st034304|FD-Score:2.97|P-value:8.81E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.22|P-value:3.60E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.57|P-value:1.52E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.16|P-value:6.68E-6 ID:SGTC_1917|Compound:st066507|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.08|P-value:5.98E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-3.62|P-value:1.26E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-4.04|P-value:2.12E-5 ID:SGTC_2297|Compound:7971671|FD-Score:-3.91|P-value:3.67E-5 ID:SGTC_2301|Compound:7769476|FD-Score:-7.15|P-value:1.41E-13 ID:SGTC_2405|Compound:ticlatone|FD-Score:2.98|P-value:8.66E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.41|P-value:1.73E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.01|P-value:1.37E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.71|P-value:4.32E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:2.97|P-value:8.83E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.17|P-value:4.28E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.71|P-value:4.91E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.97|P-value:1.60E-5 ID:SGTC_2980|Compound:9014342|FD-Score:3.00|P-value:7.82E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.45|P-value:2.40E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.00|P-value:7.81E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.10|P-value:5.02E-8 ID:SGTC_117|Compound:lucigenin|FD-Score:6.85|P-value:5.29E-13 ID:SGTC_118|Compound:0083-0097|FD-Score:3.74|P-value:4.33E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.17|P-value:6.91E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.28|P-value:3.85E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.05|P-value:6.69E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.00|P-value:2.48E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:4.27|P-value:4.06E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:7.59|P-value:1.56E-15 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:7.87|P-value:1.44E-16 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:9.38|P-value:1.14E-22 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.73|P-value:4.65E-16 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.94|P-value:2.60E-13 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:9.28|P-value:2.99E-22 ID:SGTC_309|Compound:k408-0051|FD-Score:3.93|P-value:1.91E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.56|P-value:9.35E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.19|P-value:3.93E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:6.15|P-value:7.58E-11 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.24|P-value:5.28E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:5.20|P-value:2.93E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.90|P-value:3.66E-13 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.89|P-value:2.33E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:5.48|P-value:5.65E-9 ID:SGTC_697|Compound:0508-1955|FD-Score:4.66|P-value:5.56E-7 ID:SGTC_715|Compound:0868-0130|FD-Score:5.07|P-value:6.18E-8 ID:SGTC_720|Compound:r052-1644|FD-Score:3.91|P-value:2.11E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:3.55|P-value:9.58E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:4.07|P-value:9.97E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:2.97|P-value:8.88E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.34|P-value:3.65E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.29|P-value:2.76E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.16|P-value:4.50E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.03|P-value:7.16E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.50|P-value:1.21E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.61|P-value:2.44E-9 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.22|P-value:3.51E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.34|P-value:3.69E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.11|P-value:8.48E-6 ID:SGTC_1722|Compound:st034304|FD-Score:2.97|P-value:8.81E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.22|P-value:3.60E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.57|P-value:1.52E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.16|P-value:6.68E-6 ID:SGTC_1917|Compound:st066507|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.08|P-value:5.98E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-3.62|P-value:1.26E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-4.04|P-value:2.12E-5 ID:SGTC_2297|Compound:7971671|FD-Score:-3.91|P-value:3.67E-5 ID:SGTC_2301|Compound:7769476|FD-Score:-7.15|P-value:1.41E-13 ID:SGTC_2405|Compound:ticlatone|FD-Score:2.98|P-value:8.66E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.41|P-value:1.73E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.01|P-value:1.37E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.71|P-value:4.32E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:2.97|P-value:8.83E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.17|P-value:4.28E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.71|P-value:4.91E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.97|P-value:1.60E-5 ID:SGTC_2980|Compound:9014342|FD-Score:3.00|P-value:7.82E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.45|P-value:2.40E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.00|P-value:7.81E-4

Top fitness defect scores for YCR063W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_240 5hr heat shock (37°C) + Streptovitacin 9.38 300.0 µM 1.14E-22 heat shock/prefoldin 7 3.44E-21
2 SGTC_243 5hr heat shock (37°C) + splitomicin 9.28 1.0 µM 2.99E-22 heat shock/prefoldin 6 8.52E-21
3 SGTC_239 5hr heat shock (37°C) + mitomycin C 7.87 819.4 µM 1.44E-16 heat shock/prefoldin 7 1.76E-15
4 SGTC_241 5hr heat shock (37°C) + cantharidin 7.73 155.4 µM 4.65E-16 cell wall signaling 19 5.26E-15
5 SGTC_238 5hr heat shock (37°C) + nsc-638432 7.59 8.9 µM 1.56E-15 heat shock/prefoldin 11 1.63E-14
6 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 6.94 4.6 µM 2.60E-13 heat shock/prefoldin 10 1.94E-12
7 SGTC_453 enantio-paf c16 6.90 10.0 µM 3.66E-13 26 2.67E-12
8 SGTC_117 lucigenin 6.85 22.1 µM 5.29E-13 redox potentiating 31 3.78E-12
9 SGTC_422 cantharidin 6.15 155.0 µM 7.58E-11 cell wall signaling 18 3.89E-10
10 SGTC_1597 7-benzyloxygramine 5.61 47.5 µM 2.44E-9 calcium & mitochondrial duress 56 9.95E-9
11 SGTC_502 l-744,832 5.48 89.3 µM 5.65E-9 calcium & mitochondrial duress 14 2.17E-8
12 SGTC_451 chloral hydrate 5.20 100.0 µM 2.93E-8 23 1.01E-7
13 SGTC_58 0957-0393 5.10 9.0 µM 5.02E-8 cell wall 274 1.67E-7
14 SGTC_715 0868-0130 5.07 184.0 µM 6.18E-8 5 2.02E-7
15 SGTC_2582 agaric acid 4.71 100.0 µM 4.32E-7 redox potentiating 241 1.24E-6
16 SGTC_697 0508-1955 4.66 564.0 µM 5.56E-7 20 1.57E-6
17 SGTC_351 caspofungin 4.56 25.0 nM 9.35E-7 cell wall 69 2.55E-6
18 SGTC_138 0850-0259 4.28 40.9 µM 3.85E-6 cell wall 176 9.50E-6
19 SGTC_202 3594-0513 4.27 34.8 µM 4.06E-6 PDR1 7 9.99E-6
20 SGTC_1873 rapamycin 4.16 2.0 nM 6.68E-6 64 1.59E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.408 4.25E-135 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.401 4.91E-130 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.379 4.37E-115 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.284 2.09E-63 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.262 8.36E-54 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.252 1.44E-49 YCR064C_d YCR064C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
0.251 1.82E-49 YKL007W CAP1 hom Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
0.251 3.00E-49 YEL003W GIM4 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.250 5.07E-49 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.242 6.79E-46 YNL098C RAS2 hom GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.236 7.15E-44 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.230 1.44E-41 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.214 4.56E-36 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.196 2.57E-30 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.190 1.06E-28 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO