YCR066W / RAD18

E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR066W / RAD18

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.89|P-value:2.07E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:7.17|P-value:3.56E-11 ID:SGTC_117|Compound:lucigenin|FD-Score:5.78|P-value:7.75E-8 ID:SGTC_133|Compound:0724-0033|FD-Score:5.87|P-value:4.76E-8 ID:SGTC_153|Compound:4077-0274|FD-Score:4.11|P-value:9.59E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.43|P-value:8.81E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.30|P-value:4.72E-5 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.91|P-value:1.96E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.81|P-value:2.57E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:12.60|P-value:1.77E-30 ID:SGTC_321|Compound:4534-3890|FD-Score:4.00|P-value:1.44E-4 ID:SGTC_489|Compound:paxilline|FD-Score:4.38|P-value:3.51E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.76|P-value:3.27E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.95|P-value:1.69E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:3.58|P-value:5.89E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:11.90|P-value:1.44E-27 ID:SGTC_559|Compound:1550-0661|FD-Score:3.65|P-value:4.65E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:4.68|P-value:1.08E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:3.66|P-value:4.50E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:4.85|P-value:5.33E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:4.50|P-value:2.24E-5 ID:SGTC_712|Compound:r015-0009|FD-Score:3.48|P-value:7.86E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:3.84|P-value:2.43E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:4.98|P-value:3.05E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:3.59|P-value:5.64E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:5.03|P-value:2.50E-6 ID:SGTC_753|Compound:1322-0200|FD-Score:6.00|P-value:2.54E-8 ID:SGTC_809|Compound:3998-0021|FD-Score:4.93|P-value:3.75E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.41|P-value:9.87E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.79|P-value:2.89E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.70|P-value:3.92E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.61|P-value:5.30E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.67|P-value:4.40E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.74|P-value:3.49E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.85|P-value:5.26E-6 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.66|P-value:4.43E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.49|P-value:7.16E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.80|P-value:6.53E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:6.62|P-value:9.09E-10 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.81|P-value:6.18E-6 ID:SGTC_1794|Compound:st048918|FD-Score:5.08|P-value:1.98E-6 ID:SGTC_1812|Compound:st052057|FD-Score:3.94|P-value:1.77E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.81|P-value:2.73E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:10.90|P-value:2.08E-23 ID:SGTC_1896|Compound:st058441|FD-Score:4.17|P-value:7.67E-5 ID:SGTC_1936|Compound:st074407|FD-Score:8.33|P-value:1.81E-14 ID:SGTC_1961|Compound:st076550|FD-Score:7.07|P-value:6.50E-11 ID:SGTC_1970|Compound:st070122|FD-Score:4.44|P-value:2.73E-5 ID:SGTC_2011|Compound:4012611|FD-Score:3.87|P-value:2.24E-4 ID:SGTC_2031|Compound:5180455|FD-Score:3.66|P-value:4.49E-4 ID:SGTC_2099|Compound:5377090|FD-Score:6.89|P-value:1.89E-10 ID:SGTC_2197|Compound:6588183|FD-Score:7.25|P-value:2.20E-11 ID:SGTC_2211|Compound:7117706|FD-Score:-3.47|P-value:7.76E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.59|P-value:5.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_2358|Compound:9037157|FD-Score:4.96|P-value:3.40E-6 ID:SGTC_2364|Compound:9055074|FD-Score:-3.43|P-value:8.66E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.78|P-value:3.01E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.49|P-value:7.80E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.54|P-value:1.92E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.61|P-value:5.21E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.57|P-value:5.99E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:11.50|P-value:4.30E-26 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.71|P-value:3.83E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:5.27|P-value:8.46E-7 ID:SGTC_2801|Compound:7907800|FD-Score:3.78|P-value:2.97E-4 ID:SGTC_2849|Compound:9019321|FD-Score:4.38|P-value:3.46E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.54|P-value:6.62E-4 ID:SGTC_3093|Compound:9117344|FD-Score:6.04|P-value:2.01E-8 ID:SGTC_3124|Compound:9127567|FD-Score:13.80|P-value:1.38E-36 ID:SGTC_3132|Compound:9126706|FD-Score:5.98|P-value:2.80E-8 ID:SGTC_3134|Compound:9127454|FD-Score:-3.95|P-value:1.55E-4 ID:SGTC_3136|Compound:9095672|FD-Score:5.36|P-value:5.66E-7 ID:SGTC_3254|Compound:9136651|FD-Score:4.96|P-value:3.41E-6 ID:SGTC_35|Compound:0133-0054|FD-Score:3.89|P-value:2.07E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:7.17|P-value:3.56E-11 ID:SGTC_117|Compound:lucigenin|FD-Score:5.78|P-value:7.75E-8 ID:SGTC_133|Compound:0724-0033|FD-Score:5.87|P-value:4.76E-8 ID:SGTC_153|Compound:4077-0274|FD-Score:4.11|P-value:9.59E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.43|P-value:8.81E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.30|P-value:4.72E-5 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.91|P-value:1.96E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.81|P-value:2.57E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:12.60|P-value:1.77E-30 ID:SGTC_321|Compound:4534-3890|FD-Score:4.00|P-value:1.44E-4 ID:SGTC_489|Compound:paxilline|FD-Score:4.38|P-value:3.51E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.76|P-value:3.27E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.95|P-value:1.69E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:3.58|P-value:5.89E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:11.90|P-value:1.44E-27 ID:SGTC_559|Compound:1550-0661|FD-Score:3.65|P-value:4.65E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:4.68|P-value:1.08E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:3.66|P-value:4.50E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:4.85|P-value:5.33E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:4.50|P-value:2.24E-5 ID:SGTC_712|Compound:r015-0009|FD-Score:3.48|P-value:7.86E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:3.84|P-value:2.43E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:4.98|P-value:3.05E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:3.59|P-value:5.64E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:5.03|P-value:2.50E-6 ID:SGTC_753|Compound:1322-0200|FD-Score:6.00|P-value:2.54E-8 ID:SGTC_809|Compound:3998-0021|FD-Score:4.93|P-value:3.75E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.41|P-value:9.87E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.79|P-value:2.89E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.70|P-value:3.92E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.61|P-value:5.30E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.67|P-value:4.40E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.74|P-value:3.49E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.85|P-value:5.26E-6 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.66|P-value:4.43E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.49|P-value:7.16E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.80|P-value:6.53E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:6.62|P-value:9.09E-10 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.81|P-value:6.18E-6 ID:SGTC_1794|Compound:st048918|FD-Score:5.08|P-value:1.98E-6 ID:SGTC_1812|Compound:st052057|FD-Score:3.94|P-value:1.77E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.81|P-value:2.73E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:10.90|P-value:2.08E-23 ID:SGTC_1896|Compound:st058441|FD-Score:4.17|P-value:7.67E-5 ID:SGTC_1936|Compound:st074407|FD-Score:8.33|P-value:1.81E-14 ID:SGTC_1961|Compound:st076550|FD-Score:7.07|P-value:6.50E-11 ID:SGTC_1970|Compound:st070122|FD-Score:4.44|P-value:2.73E-5 ID:SGTC_2011|Compound:4012611|FD-Score:3.87|P-value:2.24E-4 ID:SGTC_2031|Compound:5180455|FD-Score:3.66|P-value:4.49E-4 ID:SGTC_2099|Compound:5377090|FD-Score:6.89|P-value:1.89E-10 ID:SGTC_2197|Compound:6588183|FD-Score:7.25|P-value:2.20E-11 ID:SGTC_2211|Compound:7117706|FD-Score:-3.47|P-value:7.76E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.59|P-value:5.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_2358|Compound:9037157|FD-Score:4.96|P-value:3.40E-6 ID:SGTC_2364|Compound:9055074|FD-Score:-3.43|P-value:8.66E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.78|P-value:3.01E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.49|P-value:7.80E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.54|P-value:1.92E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.61|P-value:5.21E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.57|P-value:5.99E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:11.50|P-value:4.30E-26 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.71|P-value:3.83E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:5.27|P-value:8.46E-7 ID:SGTC_2801|Compound:7907800|FD-Score:3.78|P-value:2.97E-4 ID:SGTC_2849|Compound:9019321|FD-Score:4.38|P-value:3.46E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.54|P-value:6.62E-4 ID:SGTC_3093|Compound:9117344|FD-Score:6.04|P-value:2.01E-8 ID:SGTC_3124|Compound:9127567|FD-Score:13.80|P-value:1.38E-36 ID:SGTC_3132|Compound:9126706|FD-Score:5.98|P-value:2.80E-8 ID:SGTC_3134|Compound:9127454|FD-Score:-3.95|P-value:1.55E-4 ID:SGTC_3136|Compound:9095672|FD-Score:5.36|P-value:5.66E-7 ID:SGTC_3254|Compound:9136651|FD-Score:4.96|P-value:3.41E-6

Top fitness defect scores for YCR066W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3124 9127567 13.80 49.5 µM 1.38E-36 DNA damage response 1 8.04E-44
2 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 12.60 44.4 nM 1.77E-30 DNA damage response 11 1.89E-36
3 SGTC_550 4123-0385 11.90 25.7 µM 1.44E-27 TSC3-RPN4 2 6.15E-33
4 SGTC_2535 4',4'-dimethoxydalbergione 11.50 51.2 µM 4.30E-26 DNA damage response 2 3.72E-31
5 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 10.90 62.6 µM 2.08E-23 TSC3-RPN4 3 6.45E-28
6 SGTC_1936 st074407 8.33 74.3 µM 1.81E-14 DNA damage response 3 3.92E-17
7 SGTC_2197 6588183 7.25 93.1 µM 2.20E-11 7 2.05E-13
8 SGTC_93 0109-0045 7.17 59.2 µM 3.56E-11 DNA damage response 3 3.66E-13
9 SGTC_1961 st076550 7.07 68.7 µM 6.50E-11 2 7.56E-13
10 SGTC_2099 5377090 6.89 177.4 µM 1.89E-10 2 2.74E-12
11 SGTC_1425 4048-4433 6.62 7.3 µM 9.09E-10 copper-dependent oxidative stress 4 1.81E-11
12 SGTC_3093 9117344 6.04 49.5 µM 2.01E-8 60S ribosome export 2 7.53E-10
13 SGTC_753 1322-0200 6.00 289.0 µM 2.54E-8 1 1.00E-9
14 SGTC_3132 9126706 5.98 49.5 µM 2.80E-8 4 1.12E-9
15 SGTC_133 0724-0033 5.87 378.9 µM 4.76E-8 7 2.13E-9
16 SGTC_117 lucigenin 5.78 22.1 µM 7.75E-8 redox potentiating 41 3.82E-9
17 SGTC_3136 9095672 5.36 49.5 µM 5.66E-7 5 4.17E-8
18 SGTC_2677 mitomycin C 5.27 100.0 µM 8.46E-7 DNA damage response 9 6.77E-8
19 SGTC_1794 st048918 5.08 76.9 µM 1.98E-6 4 1.88E-7
20 SGTC_751 0833-0568 5.03 350.0 µM 2.50E-6 5 2.49E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.384 1.95E-118 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.286 4.58E-64 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.274 5.82E-59 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.261 1.94E-53 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.233 1.19E-42 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.226 2.85E-40 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.210 1.07E-34 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.210 1.10E-34 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.169 7.02E-23 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.146 2.13E-17 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.134 8.18E-15 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.132 1.60E-14 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.128 8.24E-14 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.126 2.12E-13 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.126 2.46E-13 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein