YCR067C / SED4

Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR067C / SED4

Click on Significant Values for Screen Details ID:SGTC_112|Compound:phenothiazine|FD-Score:3.44|P-value:2.66E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.11|P-value:8.69E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:3.94|P-value:3.61E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.12|P-value:8.57E-4 ID:SGTC_489|Compound:paxilline|FD-Score:4.15|P-value:1.47E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:5.19|P-value:8.81E-8 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.07|P-value:2.11E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:4.34|P-value:6.22E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:3.36|P-value:3.53E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.41|P-value:4.51E-6 ID:SGTC_901|Compound:1112-0062|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.46|P-value:2.50E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-4.05|P-value:2.31E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.34|P-value:6.36E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.70|P-value:9.75E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.25|P-value:5.26E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.28|P-value:4.84E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.09|P-value:9.48E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:5.48|P-value:1.78E-8 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.97|P-value:3.21E-5 ID:SGTC_1443|Compound:4112-3303|FD-Score:4.03|P-value:2.48E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.32|P-value:4.18E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.09|P-value:9.28E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.45|P-value:2.55E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.40|P-value:3.18E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.64|P-value:1.24E-4 ID:SGTC_2067|Compound:5224629|FD-Score:-3.38|P-value:3.36E-4 ID:SGTC_2537|Compound:larixol|FD-Score:-3.29|P-value:4.68E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.36|P-value:3.53E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.31|P-value:4.41E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.25|P-value:5.44E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:-4.51|P-value:2.92E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:5.86|P-value:1.81E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.15|P-value:7.67E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.89|P-value:4.55E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.27|P-value:5.02E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.23|P-value:1.07E-5 ID:SGTC_2807|Compound:7945238|FD-Score:4.43|P-value:4.04E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.33|P-value:6.55E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.73|P-value:8.64E-5 ID:SGTC_2942|Compound:9052777|FD-Score:-3.64|P-value:1.25E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.10|P-value:9.04E-4 ID:SGTC_3043|Compound:9092303|FD-Score:3.29|P-value:4.60E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.85|P-value:5.23E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.44|P-value:2.66E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.11|P-value:8.69E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:3.94|P-value:3.61E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.12|P-value:8.57E-4 ID:SGTC_489|Compound:paxilline|FD-Score:4.15|P-value:1.47E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:5.19|P-value:8.81E-8 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.07|P-value:2.11E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:4.34|P-value:6.22E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:3.36|P-value:3.53E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.41|P-value:4.51E-6 ID:SGTC_901|Compound:1112-0062|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.46|P-value:2.50E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-4.05|P-value:2.31E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.34|P-value:6.36E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.70|P-value:9.75E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.25|P-value:5.26E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.28|P-value:4.84E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.09|P-value:9.48E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:5.48|P-value:1.78E-8 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.97|P-value:3.21E-5 ID:SGTC_1443|Compound:4112-3303|FD-Score:4.03|P-value:2.48E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.32|P-value:4.18E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.09|P-value:9.28E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.45|P-value:2.55E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.40|P-value:3.18E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.64|P-value:1.24E-4 ID:SGTC_2067|Compound:5224629|FD-Score:-3.38|P-value:3.36E-4 ID:SGTC_2537|Compound:larixol|FD-Score:-3.29|P-value:4.68E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.36|P-value:3.53E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.31|P-value:4.41E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.25|P-value:5.44E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:-4.51|P-value:2.92E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:5.86|P-value:1.81E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.15|P-value:7.67E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.89|P-value:4.55E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.27|P-value:5.02E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.23|P-value:1.07E-5 ID:SGTC_2807|Compound:7945238|FD-Score:4.43|P-value:4.04E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.33|P-value:6.55E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.73|P-value:8.64E-5 ID:SGTC_2942|Compound:9052777|FD-Score:-3.64|P-value:1.25E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.10|P-value:9.04E-4 ID:SGTC_3043|Compound:9092303|FD-Score:3.29|P-value:4.60E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.85|P-value:5.23E-5

Top fitness defect scores for YCR067C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2638 epoxomicin 5.86 18.0 µM 1.81E-9 7 2.26E-9
2 SGTC_1341 1486-1360 5.48 280.0 µM 1.78E-8 3 2.16E-8
3 SGTC_503 tyrphostin 8 5.19 294.0 µM 8.81E-8 TSC3-RPN4 10 1.05E-7
4 SGTC_2807 7945238 4.43 52.0 µM 4.04E-6 14 4.62E-6
5 SGTC_813 k007-0158 4.41 173.0 µM 4.51E-6 fatty acid desaturase (OLE1) 32 5.14E-6
6 SGTC_551 k048-0028 4.34 34.9 µM 6.22E-6 8 7.06E-6
7 SGTC_1055 3448-0672 4.34 86.0 µM 6.36E-6 22 7.23E-6
8 SGTC_2834 9001755 4.33 58.4 µM 6.55E-6 25 7.44E-6
9 SGTC_489 paxilline 4.15 115.0 µM 1.47E-5 PDR1 11 1.65E-5
10 SGTC_511 blebbistatin 4.07 171.0 µM 2.11E-5 DNA intercalators 20 2.37E-5
11 SGTC_1443 4112-3303 4.03 14.0 µM 2.48E-5 7 2.77E-5
12 SGTC_1430 4049-0248 3.97 69.1 µM 3.21E-5 19 3.58E-5
13 SGTC_343 4130-2827 3.94 29.8 µM 3.61E-5 33 4.02E-5
14 SGTC_2752 diclazuril 3.89 33.1 µM 4.55E-5 120 5.05E-5
15 SGTC_3059 1327-0034 3.85 3.0 µM 5.23E-5 54 5.80E-5
16 SGTC_2933 9026794 3.73 32.5 µM 8.64E-5 25 9.52E-5
17 SGTC_1059 3448-6867 3.70 171.0 µM 9.75E-5 17 1.07E-4
18 SGTC_2066 5222451 3.64 176.0 µM 1.24E-4 heme biosynthesis & mitochondrial translocase 40 1.37E-4
19 SGTC_1837 st055472 3.45 66.5 µM 2.55E-4 35 2.78E-4
20 SGTC_112 phenothiazine 3.44 287.2 µM 2.66E-4 unfolded protein response 24 2.89E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.106 9.15E-10 YGR250C YGR250C hom Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.100 7.51E-9 YLL033W IRC19 hom Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.096 2.59E-8 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.092 8.33E-8 YML100W-A_p YML100W-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.091 1.33E-7 YLL026W HSP104 hom Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.091 1.44E-7 YGR141W VPS62 hom Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins
0.091 1.47E-7 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.087 4.62E-7 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.085 9.61E-7 YNR070W_p PDR18_p hom Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.083 1.41E-6 YOR021C SFM1 hom SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
0.083 1.71E-6 YNL235C_d YNL235C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
0.082 1.93E-6 YCL064C CHA1 hom Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
0.079 4.39E-6 YOL032W OPI10 hom Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
0.078 5.61E-6 YKL040C NFU1 hom Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria
0.078 6.88E-6 YKR041W YKR041W hom Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking