YCR081W / SRB8

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR081W / SRB8

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:3.55|P-value:3.91E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.41|P-value:1.20E-7 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.71|P-value:1.90E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.20|P-value:3.54E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.37|P-value:1.46E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:4.01|P-value:7.36E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.35|P-value:1.60E-7 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:4.10|P-value:5.26E-5 ID:SGTC_489|Compound:paxilline|FD-Score:4.21|P-value:3.29E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:3.46|P-value:5.32E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.09|P-value:5.46E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.38|P-value:6.16E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.94|P-value:1.39E-6 ID:SGTC_729|Compound:4092-0585|FD-Score:3.98|P-value:8.11E-5 ID:SGTC_833|Compound:3852-0053|FD-Score:3.28|P-value:9.73E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.84|P-value:1.21E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.89|P-value:1.79E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:3.73|P-value:2.09E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.44|P-value:5.60E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.28|P-value:8.47E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.33|P-value:1.77E-12 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.73|P-value:2.10E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.41|P-value:1.45E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:7.71|P-value:1.26E-13 ID:SGTC_1134|Compound:3477-0009|FD-Score:-4.72|P-value:3.23E-6 ID:SGTC_1241|Compound:0364-0022|FD-Score:4.00|P-value:7.68E-5 ID:SGTC_1320|Compound:1313-0481|FD-Score:-3.43|P-value:5.11E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.94|P-value:9.40E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.60|P-value:2.85E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.50|P-value:4.64E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:-3.38|P-value:6.15E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:4.93|P-value:1.47E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:5.53|P-value:7.76E-8 ID:SGTC_1644|Compound:st010134|FD-Score:3.38|P-value:6.92E-4 ID:SGTC_1730|Compound:st033808|FD-Score:4.11|P-value:5.01E-5 ID:SGTC_1733|Compound:st037357|FD-Score:-4.98|P-value:9.70E-7 ID:SGTC_1739|Compound:st037196|FD-Score:3.61|P-value:3.20E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-3.89|P-value:9.78E-5 ID:SGTC_1992|Compound:st073643|FD-Score:3.44|P-value:5.71E-4 ID:SGTC_2083|Compound:5306046|FD-Score:5.05|P-value:8.29E-7 ID:SGTC_2087|Compound:5276107|FD-Score:5.21|P-value:3.85E-7 ID:SGTC_2102|Compound:5377816|FD-Score:4.55|P-value:8.26E-6 ID:SGTC_2297|Compound:7971671|FD-Score:-3.44|P-value:4.90E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.86|P-value:1.30E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.38|P-value:7.03E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.04|P-value:6.62E-5 ID:SGTC_2421|Compound:5690543|FD-Score:-3.47|P-value:4.52E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.30|P-value:8.96E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.72|P-value:3.79E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-5.09|P-value:5.75E-7 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.34|P-value:8.02E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.78|P-value:1.76E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.09|P-value:5.27E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.26|P-value:9.07E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.39|P-value:6.72E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-3.64|P-value:2.43E-4 ID:SGTC_2812|Compound:7970281|FD-Score:5.04|P-value:8.70E-7 ID:SGTC_2822|Compound:7994718|FD-Score:-3.87|P-value:1.09E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.80|P-value:1.59E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.98|P-value:6.93E-5 ID:SGTC_3099|Compound:9120367|FD-Score:3.35|P-value:7.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.85|P-value:2.10E-6 ID:SGTC_3133|Compound:9127736|FD-Score:4.96|P-value:1.28E-6 ID:SGTC_3144|Compound:9087057|FD-Score:5.09|P-value:6.85E-7 ID:SGTC_3254|Compound:9136651|FD-Score:3.67|P-value:2.58E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.87|P-value:1.64E-6 ID:SGTC_3291|Compound:9121177|FD-Score:6.94|P-value:2.21E-11 ID:SGTC_3293|Compound:9120939|FD-Score:-3.94|P-value:8.29E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.55|P-value:3.91E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.41|P-value:1.20E-7 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.71|P-value:1.90E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.20|P-value:3.54E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.37|P-value:1.46E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:4.01|P-value:7.36E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.35|P-value:1.60E-7 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:4.10|P-value:5.26E-5 ID:SGTC_489|Compound:paxilline|FD-Score:4.21|P-value:3.29E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:3.46|P-value:5.32E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.09|P-value:5.46E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.38|P-value:6.16E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.94|P-value:1.39E-6 ID:SGTC_729|Compound:4092-0585|FD-Score:3.98|P-value:8.11E-5 ID:SGTC_833|Compound:3852-0053|FD-Score:3.28|P-value:9.73E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.84|P-value:1.21E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:4.89|P-value:1.79E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:3.73|P-value:2.09E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.44|P-value:5.60E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.28|P-value:8.47E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.33|P-value:1.77E-12 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.73|P-value:2.10E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.41|P-value:1.45E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:7.71|P-value:1.26E-13 ID:SGTC_1134|Compound:3477-0009|FD-Score:-4.72|P-value:3.23E-6 ID:SGTC_1241|Compound:0364-0022|FD-Score:4.00|P-value:7.68E-5 ID:SGTC_1320|Compound:1313-0481|FD-Score:-3.43|P-value:5.11E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.94|P-value:9.40E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.60|P-value:2.85E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.50|P-value:4.64E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:-3.38|P-value:6.15E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:4.93|P-value:1.47E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:5.53|P-value:7.76E-8 ID:SGTC_1644|Compound:st010134|FD-Score:3.38|P-value:6.92E-4 ID:SGTC_1730|Compound:st033808|FD-Score:4.11|P-value:5.01E-5 ID:SGTC_1733|Compound:st037357|FD-Score:-4.98|P-value:9.70E-7 ID:SGTC_1739|Compound:st037196|FD-Score:3.61|P-value:3.20E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-3.89|P-value:9.78E-5 ID:SGTC_1992|Compound:st073643|FD-Score:3.44|P-value:5.71E-4 ID:SGTC_2083|Compound:5306046|FD-Score:5.05|P-value:8.29E-7 ID:SGTC_2087|Compound:5276107|FD-Score:5.21|P-value:3.85E-7 ID:SGTC_2102|Compound:5377816|FD-Score:4.55|P-value:8.26E-6 ID:SGTC_2297|Compound:7971671|FD-Score:-3.44|P-value:4.90E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.86|P-value:1.30E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.38|P-value:7.03E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.04|P-value:6.62E-5 ID:SGTC_2421|Compound:5690543|FD-Score:-3.47|P-value:4.52E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.30|P-value:8.96E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.72|P-value:3.79E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-5.09|P-value:5.75E-7 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.34|P-value:8.02E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.78|P-value:1.76E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.09|P-value:5.27E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.26|P-value:9.07E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.39|P-value:6.72E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-3.64|P-value:2.43E-4 ID:SGTC_2812|Compound:7970281|FD-Score:5.04|P-value:8.70E-7 ID:SGTC_2822|Compound:7994718|FD-Score:-3.87|P-value:1.09E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.80|P-value:1.59E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.98|P-value:6.93E-5 ID:SGTC_3099|Compound:9120367|FD-Score:3.35|P-value:7.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.85|P-value:2.10E-6 ID:SGTC_3133|Compound:9127736|FD-Score:4.96|P-value:1.28E-6 ID:SGTC_3144|Compound:9087057|FD-Score:5.09|P-value:6.85E-7 ID:SGTC_3254|Compound:9136651|FD-Score:3.67|P-value:2.58E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.87|P-value:1.64E-6 ID:SGTC_3291|Compound:9121177|FD-Score:6.94|P-value:2.21E-11 ID:SGTC_3293|Compound:9120939|FD-Score:-3.94|P-value:8.29E-5

Top fitness defect scores for YCR081W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1116 jasmonic acid 7.71 1.6 µM 1.26E-13 1 6.35E-15
2 SGTC_1082 camptothecin 7.33 424.9 nM 1.77E-12 DNA damage response 14 1.17E-13
3 SGTC_3291 9121177 6.94 71.4 µM 2.21E-11 1 1.91E-12
4 SGTC_1479 0549-0158 5.53 134.0 µM 7.76E-8 5 1.58E-8
5 SGTC_2087 5276107 5.21 200.0 µM 3.85E-7 4 9.25E-8
6 SGTC_3144 9087057 5.09 49.5 µM 6.85E-7 5 1.75E-7
7 SGTC_2083 5306046 5.05 65.9 µM 8.29E-7 6 2.16E-7
8 SGTC_2812 7970281 5.04 58.4 µM 8.70E-7 6 2.28E-7
9 SGTC_3133 9127736 4.96 49.5 µM 1.28E-6 8 3.50E-7
10 SGTC_682 0kpi-0099 4.94 17.0 µM 1.39E-6 39 3.81E-7
11 SGTC_1474 0330-0125 4.93 5.3 µM 1.47E-6 13 4.06E-7
12 SGTC_912 3474-0017 4.89 2.2 µM 1.79E-6 12 5.05E-7
13 SGTC_3131 9127911 4.85 49.5 µM 2.10E-6 11 6.04E-7
14 SGTC_2517 totarol 4.72 10.0 µM 3.79E-6 10 1.16E-6
15 SGTC_2102 5377816 4.55 72.8 µM 8.26E-6 11 2.74E-6
16 SGTC_1102 cgs 12066b 4.41 26.1 µM 1.45E-5 endomembrane recycling 4 5.09E-6
17 SGTC_489 paxilline 4.21 115.0 µM 3.29E-5 PDR1 10 1.26E-5
18 SGTC_116 0155-0182 4.20 31.8 µM 3.54E-5 mitochondrial response to ROS 64 1.36E-5
19 SGTC_1730 st033808 4.11 45.1 µM 5.01E-5 26 2.00E-5
20 SGTC_478 N-arachidonylglycine 4.10 10.0 µM 5.26E-5 4 2.11E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 3.00E-9 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.101 4.46E-9 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.098 1.49E-8 YJL128C PBS2 hom MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
0.096 2.78E-8 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.090 1.79E-7 YAL030W SNC1 hom Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
0.090 1.98E-7 YPL042C SSN3 hom Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
0.088 3.21E-7 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.087 5.14E-7 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.086 5.89E-7 YBL042C FUI1 hom High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
0.086 6.88E-7 YNL196C_p SLZ1_p hom Sporulation-specific protein with a leucine zipper motif
0.084 1.20E-6 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.083 1.58E-6 YHR114W BZZ1 hom SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins
0.081 2.78E-6 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.081 2.81E-6 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.080 3.86E-6 YKL207W EMC3 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111