YDL003W / MCD1

Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL003W / MCD1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-3.75|P-value:1.17E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.37|P-value:5.27E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.25|P-value:7.25E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.48|P-value:3.49E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:4.65|P-value:2.96E-6 ID:SGTC_180|Compound:1486-1293|FD-Score:-3.32|P-value:5.74E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.45|P-value:7.33E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.25|P-value:7.25E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.20|P-value:1.12E-12 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.34|P-value:5.74E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.55|P-value:4.75E-6 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.25|P-value:7.76E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.09|P-value:3.39E-5 ID:SGTC_1763|Compound:st044482|FD-Score:3.31|P-value:6.43E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.12|P-value:3.01E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.63|P-value:2.05E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.31|P-value:1.35E-5 ID:SGTC_2254|Compound:7938975|FD-Score:3.61|P-value:2.18E-4 ID:SGTC_2339|Compound:9000433|FD-Score:3.76|P-value:1.26E-4 ID:SGTC_2393|Compound:9031399|FD-Score:-3.64|P-value:1.80E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.73|P-value:1.40E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.49|P-value:3.47E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.56|P-value:2.62E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.31|P-value:6.40E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.42|P-value:4.41E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.73|P-value:1.39E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.20|P-value:9.30E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.11|P-value:3.15E-7 ID:SGTC_2956|Compound:9076196|FD-Score:3.32|P-value:6.09E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-6.31|P-value:3.24E-10 ID:SGTC_2980|Compound:9014342|FD-Score:3.37|P-value:5.16E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.18|P-value:2.39E-5 ID:SGTC_3051|Compound:9093778|FD-Score:3.18|P-value:9.89E-4 ID:SGTC_3122|Compound:9126309|FD-Score:3.39|P-value:4.94E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.25|P-value:7.28E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.80|P-value:1.10E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.75|P-value:1.17E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.37|P-value:5.27E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.25|P-value:7.25E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.48|P-value:3.49E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:4.65|P-value:2.96E-6 ID:SGTC_180|Compound:1486-1293|FD-Score:-3.32|P-value:5.74E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.45|P-value:7.33E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-3.25|P-value:7.25E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.20|P-value:1.12E-12 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.34|P-value:5.74E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.55|P-value:4.75E-6 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.25|P-value:7.76E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.09|P-value:3.39E-5 ID:SGTC_1763|Compound:st044482|FD-Score:3.31|P-value:6.43E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.12|P-value:3.01E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.63|P-value:2.05E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.31|P-value:1.35E-5 ID:SGTC_2254|Compound:7938975|FD-Score:3.61|P-value:2.18E-4 ID:SGTC_2339|Compound:9000433|FD-Score:3.76|P-value:1.26E-4 ID:SGTC_2393|Compound:9031399|FD-Score:-3.64|P-value:1.80E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.73|P-value:1.40E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.49|P-value:3.47E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.56|P-value:2.62E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.31|P-value:6.40E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.42|P-value:4.41E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.73|P-value:1.39E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.20|P-value:9.30E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.11|P-value:3.15E-7 ID:SGTC_2956|Compound:9076196|FD-Score:3.32|P-value:6.09E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-6.31|P-value:3.24E-10 ID:SGTC_2980|Compound:9014342|FD-Score:3.37|P-value:5.16E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.18|P-value:2.39E-5 ID:SGTC_3051|Compound:9093778|FD-Score:3.18|P-value:9.89E-4 ID:SGTC_3122|Compound:9126309|FD-Score:3.39|P-value:4.94E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.25|P-value:7.28E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.80|P-value:1.10E-4

Top fitness defect scores for YDL003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_268 camptothecin 7.20 39.6 µM 1.12E-12 DNA damage response 16 3.03E-13 0.09
2 SGTC_2890 9046729 5.11 58.4 µM 3.15E-7 Golgi 13 1.58E-7 0.32
3 SGTC_172 1112-0046 4.65 2.6 µM 2.96E-6 NEO1 5 1.66E-6 0.40
4 SGTC_1109 tnp00067 4.55 928.3 nM 4.75E-6 2 2.72E-6 1.23
5 SGTC_236 1509-0011 4.45 5.3 µM 7.33E-6 22 4.29E-6 0.04
6 SGTC_2240 6690706 4.31 185.9 µM 1.35E-5 4 8.11E-6 0.16
7 SGTC_3009 9080819 4.18 71.4 µM 2.39E-5 7 1.48E-5 0.01
8 SGTC_1954 st077032 4.12 68.9 µM 3.01E-5 6 1.89E-5 0.06
9 SGTC_1289 0976-0073 4.09 69.1 µM 3.39E-5 3 2.14E-5 0.65
10 SGTC_3296 9122258 3.80 64.0 µM 1.10E-4 redox potentiating 7 7.37E-5 0.07
11 SGTC_2339 9000433 3.76 200.0 µM 1.26E-4 6 8.49E-5 0.18
12 SGTC_2881 9058953 3.73 52.0 µM 1.39E-4 7 9.42E-5 0.12
13 SGTC_2396 5342950 3.73 42.8 µM 1.40E-4 RSC complex & mRNA processing 39 9.48E-5 0.02
14 SGTC_2183 6420625 3.63 130.5 µM 2.05E-4 mitochondrial processes 19 1.41E-4 0.05
15 SGTC_2254 7938975 3.61 95.2 µM 2.18E-4 10 1.50E-4 0.03
16 SGTC_2730 imipramine 3.56 71.3 µM 2.62E-4 10 1.83E-4 0.12
17 SGTC_2611 pyromycin 3.49 23.9 µM 3.47E-4 12 2.45E-4 0.02
18 SGTC_160 3453-0671 3.48 289.7 µM 3.49E-4 5 2.46E-4 0.02
19 SGTC_2865 9030313 3.42 52.0 µM 4.41E-4 2 3.15E-4 0.22
20 SGTC_3122 9126309 3.39 49.5 µM 4.94E-4 cell wall signaling 5 3.55E-4 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.165 6.26E-22 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.148 7.48E-18 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.137 1.79E-15 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.134 6.24E-15 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.129 5.52E-14 YOR372C NDD1 het Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
0.128 9.27E-14 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.127 1.61E-13 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.125 3.18E-13 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.124 5.96E-13 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.122 1.44E-12 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.122 1.45E-12 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.120 3.16E-12 YDR217C RAD9 hom DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.116 1.86E-11 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.115 2.23E-11 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.114 3.13E-11 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes