YDL006W / PTC1

Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL006W / PTC1

Click on Significant Values for Screen Details ID:SGTC_117|Compound:lucigenin|FD-Score:6.53|P-value:7.89E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:7.67|P-value:8.74E-9 ID:SGTC_255|Compound:tunicamycin|FD-Score:-4.97|P-value:1.26E-4 ID:SGTC_269|Compound:exalamide|FD-Score:7.83|P-value:4.29E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.72|P-value:2.48E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.42|P-value:5.85E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.23|P-value:9.54E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:5.23|P-value:6.15E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:9.32|P-value:3.78E-12 ID:SGTC_727|Compound:3032-0951|FD-Score:4.63|P-value:3.37E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:6.66|P-value:5.03E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-6.62|P-value:5.33E-7 ID:SGTC_823|Compound:0417-1665|FD-Score:-4.28|P-value:8.10E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:5.35|P-value:4.29E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:5.85|P-value:8.72E-6 ID:SGTC_863|Compound:0466-0191|FD-Score:6.14|P-value:3.29E-6 ID:SGTC_878|Compound:0828-0289|FD-Score:6.56|P-value:7.17E-7 ID:SGTC_879|Compound:0831-1030|FD-Score:4.27|P-value:8.56E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:8.82|P-value:4.52E-11 ID:SGTC_1092|Compound:toremifene|FD-Score:5.15|P-value:7.82E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-4.25|P-value:8.70E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:5.21|P-value:6.56E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:4.35|P-value:6.94E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:5.48|P-value:2.84E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.28|P-value:8.38E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-4.65|P-value:3.03E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:-5.01|P-value:1.11E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:5.18|P-value:7.14E-5 ID:SGTC_1608|Compound:st001473|FD-Score:8.64|P-value:1.09E-10 ID:SGTC_1654|Compound:st012436|FD-Score:4.98|P-value:1.27E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.19|P-value:6.96E-5 ID:SGTC_1847|Compound:st056231|FD-Score:4.21|P-value:9.93E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.27|P-value:8.65E-4 ID:SGTC_1909|Compound:st060837|FD-Score:7.60|P-value:1.16E-8 ID:SGTC_1915|Compound:st067105|FD-Score:5.54|P-value:2.39E-5 ID:SGTC_1917|Compound:st066507|FD-Score:4.85|P-value:1.82E-4 ID:SGTC_2043|Compound:5101342|FD-Score:4.65|P-value:3.21E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.75|P-value:2.45E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.75|P-value:2.46E-4 ID:SGTC_2259|Compound:7964009|FD-Score:6.61|P-value:5.94E-7 ID:SGTC_2475|Compound:5812728|FD-Score:-4.25|P-value:8.61E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.27|P-value:8.69E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:5.39|P-value:3.78E-5 ID:SGTC_2845|Compound:9003024|FD-Score:-4.72|P-value:2.50E-4 ID:SGTC_2912|Compound:7964298|FD-Score:7.01|P-value:1.29E-7 ID:SGTC_2941|Compound:9056145|FD-Score:-5.43|P-value:3.10E-5 ID:SGTC_2947|Compound:9072639|FD-Score:8.30|P-value:5.22E-10 ID:SGTC_2949|Compound:9072542|FD-Score:6.18|P-value:2.81E-6 ID:SGTC_2951|Compound:9072950|FD-Score:8.64|P-value:1.06E-10 ID:SGTC_3020|Compound:9089140|FD-Score:4.31|P-value:7.87E-4 ID:SGTC_3025|Compound:9087895|FD-Score:5.06|P-value:1.01E-4 ID:SGTC_3029|Compound:9088683|FD-Score:6.12|P-value:3.48E-6 ID:SGTC_3032|Compound:9089427|FD-Score:-4.60|P-value:3.53E-4 ID:SGTC_3054|Compound:9094397|FD-Score:6.38|P-value:1.39E-6 ID:SGTC_3057|Compound:9095946|FD-Score:5.16|P-value:7.51E-5 ID:SGTC_3191|Compound:9110352|FD-Score:4.30|P-value:7.88E-4 ID:SGTC_3197|Compound:9113331|FD-Score:6.33|P-value:1.66E-6 ID:SGTC_3219|Compound:9131112|FD-Score:6.93|P-value:1.77E-7 ID:SGTC_3277|Compound:9138724|FD-Score:5.73|P-value:1.29E-5 ID:SGTC_3285|Compound:9112818|FD-Score:5.76|P-value:1.18E-5 ID:SGTC_3338|Compound:9144300|FD-Score:4.79|P-value:2.18E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:6.53|P-value:7.89E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:7.67|P-value:8.74E-9 ID:SGTC_255|Compound:tunicamycin|FD-Score:-4.97|P-value:1.26E-4 ID:SGTC_269|Compound:exalamide|FD-Score:7.83|P-value:4.29E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.72|P-value:2.48E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.42|P-value:5.85E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.23|P-value:9.54E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:5.23|P-value:6.15E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:9.32|P-value:3.78E-12 ID:SGTC_727|Compound:3032-0951|FD-Score:4.63|P-value:3.37E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:6.66|P-value:5.03E-7 ID:SGTC_758|Compound:0868-0029|FD-Score:-6.62|P-value:5.33E-7 ID:SGTC_823|Compound:0417-1665|FD-Score:-4.28|P-value:8.10E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:5.35|P-value:4.29E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:5.85|P-value:8.72E-6 ID:SGTC_863|Compound:0466-0191|FD-Score:6.14|P-value:3.29E-6 ID:SGTC_878|Compound:0828-0289|FD-Score:6.56|P-value:7.17E-7 ID:SGTC_879|Compound:0831-1030|FD-Score:4.27|P-value:8.56E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:8.82|P-value:4.52E-11 ID:SGTC_1092|Compound:toremifene|FD-Score:5.15|P-value:7.82E-5 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-4.25|P-value:8.70E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:5.21|P-value:6.56E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:4.35|P-value:6.94E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:5.48|P-value:2.84E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:4.28|P-value:8.38E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-4.65|P-value:3.03E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:-5.01|P-value:1.11E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:5.18|P-value:7.14E-5 ID:SGTC_1608|Compound:st001473|FD-Score:8.64|P-value:1.09E-10 ID:SGTC_1654|Compound:st012436|FD-Score:4.98|P-value:1.27E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.19|P-value:6.96E-5 ID:SGTC_1847|Compound:st056231|FD-Score:4.21|P-value:9.93E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.27|P-value:8.65E-4 ID:SGTC_1909|Compound:st060837|FD-Score:7.60|P-value:1.16E-8 ID:SGTC_1915|Compound:st067105|FD-Score:5.54|P-value:2.39E-5 ID:SGTC_1917|Compound:st066507|FD-Score:4.85|P-value:1.82E-4 ID:SGTC_2043|Compound:5101342|FD-Score:4.65|P-value:3.21E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.75|P-value:2.45E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.75|P-value:2.46E-4 ID:SGTC_2259|Compound:7964009|FD-Score:6.61|P-value:5.94E-7 ID:SGTC_2475|Compound:5812728|FD-Score:-4.25|P-value:8.61E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.27|P-value:8.69E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:5.39|P-value:3.78E-5 ID:SGTC_2845|Compound:9003024|FD-Score:-4.72|P-value:2.50E-4 ID:SGTC_2912|Compound:7964298|FD-Score:7.01|P-value:1.29E-7 ID:SGTC_2941|Compound:9056145|FD-Score:-5.43|P-value:3.10E-5 ID:SGTC_2947|Compound:9072639|FD-Score:8.30|P-value:5.22E-10 ID:SGTC_2949|Compound:9072542|FD-Score:6.18|P-value:2.81E-6 ID:SGTC_2951|Compound:9072950|FD-Score:8.64|P-value:1.06E-10 ID:SGTC_3020|Compound:9089140|FD-Score:4.31|P-value:7.87E-4 ID:SGTC_3025|Compound:9087895|FD-Score:5.06|P-value:1.01E-4 ID:SGTC_3029|Compound:9088683|FD-Score:6.12|P-value:3.48E-6 ID:SGTC_3032|Compound:9089427|FD-Score:-4.60|P-value:3.53E-4 ID:SGTC_3054|Compound:9094397|FD-Score:6.38|P-value:1.39E-6 ID:SGTC_3057|Compound:9095946|FD-Score:5.16|P-value:7.51E-5 ID:SGTC_3191|Compound:9110352|FD-Score:4.30|P-value:7.88E-4 ID:SGTC_3197|Compound:9113331|FD-Score:6.33|P-value:1.66E-6 ID:SGTC_3219|Compound:9131112|FD-Score:6.93|P-value:1.77E-7 ID:SGTC_3277|Compound:9138724|FD-Score:5.73|P-value:1.29E-5 ID:SGTC_3285|Compound:9112818|FD-Score:5.76|P-value:1.18E-5 ID:SGTC_3338|Compound:9144300|FD-Score:4.79|P-value:2.18E-4

Top fitness defect scores for YDL006W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_524 r073-0010 9.32 27.8 µM 3.78E-12 4 6.09E-21
2 SGTC_883 0850-0245 8.82 24.6 µM 4.52E-11 cell wall 5 5.73E-19
3 SGTC_2951 9072950 8.64 58.1 µM 1.06E-10 ERG2 2 2.74E-18
4 SGTC_1608 st001473 8.64 40.5 µM 1.09E-10 5 2.86E-18
5 SGTC_2947 9072639 8.30 7.7 µM 5.22E-10 tubulin folding & SWR complex 6 5.04E-17
6 SGTC_269 exalamide 7.83 29.0 µM 4.29E-9 ergosterol biosynthesis 2 2.37E-15
7 SGTC_245 methotrexate 7.67 289.6 µM 8.74E-9 5 8.70E-15
8 SGTC_1909 st060837 7.60 51.5 µM 1.16E-8 12 1.46E-14
9 SGTC_2912 7964298 7.01 10.9 µM 1.29E-7 cell wall 35 1.19E-12
10 SGTC_3219 9131112 6.93 49.5 µM 1.77E-7 Golgi 10 2.09E-12
11 SGTC_733 4069-0007 6.66 56.1 µM 5.03E-7 ERAD & cell cycle 32 1.40E-11
12 SGTC_2259 7964009 6.61 200.0 µM 5.94E-7 superoxide 11 1.90E-11
13 SGTC_878 0828-0289 6.56 13.7 µM 7.17E-7 11 2.68E-11
14 SGTC_117 lucigenin 6.53 22.1 µM 7.89E-7 redox potentiating 34 3.18E-11
15 SGTC_3054 9094397 6.38 49.5 µM 1.39E-6 fatty acid desaturase (OLE1) 6 8.88E-11
16 SGTC_3197 9113331 6.33 49.5 µM 1.66E-6 4 1.23E-10
17 SGTC_2949 9072542 6.18 2.2 µM 2.81E-6 TSC3-RPN4 5 3.20E-10
18 SGTC_863 0466-0191 6.14 129.0 µM 3.29E-6 cell wall 28 4.26E-10
19 SGTC_3029 9088683 6.12 49.5 µM 3.48E-6 5 4.72E-10
20 SGTC_825 1988-2224 5.85 89.6 µM 8.72E-6 cell wall signaling 17 2.50E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.252 1.26E-49 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.140 4.02E-16 YDR159W SAC3 hom Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.139 6.04E-16 YAL021C CCR4 hom Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
0.128 1.06E-13 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.126 2.42E-13 YDR200C VPS64 hom Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
0.126 3.14E-13 YDR526C_d YDR526C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.124 5.26E-13 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.123 7.80E-13 YDR389W SAC7 hom GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.121 2.21E-12 YDR293C SSD1 hom Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
0.121 1.89E-12 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.120 2.84E-12 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.120 3.03E-12 YLR268W SEC22 hom R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
0.118 7.98E-12 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.116 1.57E-11 YEL044W IES6 hom Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
0.113 5.35E-11 YOL072W THP1 hom Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding