YDL007W / RPT2

One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL007W / RPT2

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-3.21|P-value:5.33E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.94|P-value:2.80E-5 ID:SGTC_186|Compound:3455-3465|FD-Score:3.50|P-value:1.33E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.13|P-value:1.19E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.39|P-value:2.45E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:3.15|P-value:5.03E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.64|P-value:7.46E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.90|P-value:2.43E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.38|P-value:2.16E-4 ID:SGTC_462|Compound:ag-879|FD-Score:5.13|P-value:5.00E-8 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.20|P-value:4.17E-4 ID:SGTC_490|Compound:phenamil|FD-Score:5.59|P-value:3.24E-9 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.74|P-value:6.53E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.19|P-value:4.42E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.19|P-value:5.63E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.11|P-value:7.53E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.63|P-value:1.05E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.29|P-value:2.95E-8 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.14|P-value:1.14E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.62|P-value:5.41E-12 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.46|P-value:2.07E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.23|P-value:7.70E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.96|P-value:1.90E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.87|P-value:3.89E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.23|P-value:7.58E-6 ID:SGTC_1725|Compound:st036284|FD-Score:3.54|P-value:1.12E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.73|P-value:6.53E-7 ID:SGTC_1737|Compound:st035557|FD-Score:-5.13|P-value:7.62E-8 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.28|P-value:4.14E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.98|P-value:1.73E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.79|P-value:3.12E-7 ID:SGTC_2044|Compound:5102272|FD-Score:2.97|P-value:9.59E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.00|P-value:2.19E-5 ID:SGTC_2124|Compound:5246320|FD-Score:5.60|P-value:3.07E-9 ID:SGTC_2159|Compound:5658611|FD-Score:3.34|P-value:2.45E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.21|P-value:5.29E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.26|P-value:4.44E-4 ID:SGTC_2344|Compound:7990960|FD-Score:5.84|P-value:6.83E-10 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-4.01|P-value:2.12E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.19|P-value:5.64E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:5.84|P-value:6.77E-10 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.07|P-value:8.69E-4 ID:SGTC_2937|Compound:9044753|FD-Score:-3.22|P-value:5.19E-4 ID:SGTC_2962|Compound:9082311|FD-Score:4.08|P-value:1.11E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-6.03|P-value:3.09E-10 ID:SGTC_3062|Compound:9077494|FD-Score:-6.78|P-value:1.72E-12 ID:SGTC_3080|Compound:9117754|FD-Score:3.19|P-value:4.35E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.29|P-value:2.99E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.28|P-value:4.06E-4 ID:SGTC_3142|Compound:9086498|FD-Score:6.26|P-value:4.02E-11 ID:SGTC_3283|Compound:9109332|FD-Score:-3.86|P-value:4.02E-5 ID:SGTC_3338|Compound:9144300|FD-Score:-3.39|P-value:2.75E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.21|P-value:5.33E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.94|P-value:2.80E-5 ID:SGTC_186|Compound:3455-3465|FD-Score:3.50|P-value:1.33E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.13|P-value:1.19E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.39|P-value:2.45E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:3.15|P-value:5.03E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.64|P-value:7.46E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.90|P-value:2.43E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.38|P-value:2.16E-4 ID:SGTC_462|Compound:ag-879|FD-Score:5.13|P-value:5.00E-8 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.20|P-value:4.17E-4 ID:SGTC_490|Compound:phenamil|FD-Score:5.59|P-value:3.24E-9 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.74|P-value:6.53E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.19|P-value:4.42E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.19|P-value:5.63E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.11|P-value:7.53E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.63|P-value:1.05E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.29|P-value:2.95E-8 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.14|P-value:1.14E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.62|P-value:5.41E-12 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.46|P-value:2.07E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.23|P-value:7.70E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.96|P-value:1.90E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.87|P-value:3.89E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.23|P-value:7.58E-6 ID:SGTC_1725|Compound:st036284|FD-Score:3.54|P-value:1.12E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.73|P-value:6.53E-7 ID:SGTC_1737|Compound:st035557|FD-Score:-5.13|P-value:7.62E-8 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.28|P-value:4.14E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.98|P-value:1.73E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.79|P-value:3.12E-7 ID:SGTC_2044|Compound:5102272|FD-Score:2.97|P-value:9.59E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.00|P-value:2.19E-5 ID:SGTC_2124|Compound:5246320|FD-Score:5.60|P-value:3.07E-9 ID:SGTC_2159|Compound:5658611|FD-Score:3.34|P-value:2.45E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.21|P-value:5.29E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.26|P-value:4.44E-4 ID:SGTC_2344|Compound:7990960|FD-Score:5.84|P-value:6.83E-10 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-4.01|P-value:2.12E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.19|P-value:5.64E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:5.84|P-value:6.77E-10 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.07|P-value:8.69E-4 ID:SGTC_2937|Compound:9044753|FD-Score:-3.22|P-value:5.19E-4 ID:SGTC_2962|Compound:9082311|FD-Score:4.08|P-value:1.11E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-6.03|P-value:3.09E-10 ID:SGTC_3062|Compound:9077494|FD-Score:-6.78|P-value:1.72E-12 ID:SGTC_3080|Compound:9117754|FD-Score:3.19|P-value:4.35E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.29|P-value:2.99E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.28|P-value:4.06E-4 ID:SGTC_3142|Compound:9086498|FD-Score:6.26|P-value:4.02E-11 ID:SGTC_3283|Compound:9109332|FD-Score:-3.86|P-value:4.02E-5 ID:SGTC_3338|Compound:9144300|FD-Score:-3.39|P-value:2.75E-4

Top fitness defect scores for YDL007W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3142 9086498 6.26 49.5 µM 4.02E-11 iron homeostasis 23 1.87E-10 0.31
2 SGTC_2575 plumbagin 5.84 510.0 nM 6.77E-10 superoxide 13 2.63E-9 0.41
3 SGTC_2344 7990960 5.84 77.0 µM 6.83E-10 19 2.65E-9 0.11
4 SGTC_2124 5246320 5.60 200.0 µM 3.07E-9 heme biosynthesis & mitochondrial translocase 18 1.08E-8 0.12
5 SGTC_490 phenamil 5.59 164.0 µM 3.24E-9 heme requiring 2 1.14E-8 0.61
6 SGTC_462 ag-879 5.13 158.0 µM 5.00E-8 5 1.47E-7 0.15
7 SGTC_2019 4016004 4.79 108.0 µM 3.12E-7 6 8.15E-7 0.12
8 SGTC_339 3091-4833 4.39 71.4 µM 2.45E-6 iron homeostasis 7 5.59E-6 0.23
9 SGTC_2962 9082311 4.08 13.2 µM 1.11E-5 10 2.30E-5 0.21
10 SGTC_2014 4013930 3.98 200.0 µM 1.73E-5 3 3.47E-5 0.11
11 SGTC_1309 pyrazolanthrone 3.96 13.4 µM 1.90E-5 9 3.79E-5 0.15
12 SGTC_431 k089-0097 3.90 107.0 µM 2.43E-5 iron homeostasis 16 4.78E-5 0.08
13 SGTC_410 α-linolenic acid 3.64 100.0 µM 7.46E-5 excess fatty acid 8 1.36E-4 0.05
14 SGTC_1725 st036284 3.54 20.6 µM 1.12E-4 7 2.00E-4 0.09
15 SGTC_186 3455-3465 3.50 67.3 µM 1.33E-4 4 2.34E-4 0.15
16 SGTC_444 curcumin 3.38 90.4 µM 2.16E-4 iron homeostasis 24 3.67E-4 0.04
17 SGTC_2159 5658611 3.34 73.8 µM 2.45E-4 11 4.14E-4 0.08
18 SGTC_3126 9127922 3.29 49.5 µM 2.99E-4 fatty acid desaturase (OLE1) 43 4.98E-4 0.04
19 SGTC_474 dl-dihydrosphingosine 3.20 5.0 µM 4.17E-4 16 6.81E-4 0.07
20 SGTC_3080 9117754 3.19 49.5 µM 4.35E-4 11 7.08E-4 3.95E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 4.45E-60 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.271 1.14E-57 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.246 1.30E-47 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.242 9.11E-46 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.237 5.13E-44 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.233 1.31E-42 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.233 1.18E-42 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.228 1.01E-40 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.216 7.45E-37 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.201 5.47E-32 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.196 2.04E-30 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.191 7.78E-29 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.180 8.28E-26 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.172 8.05E-24 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.170 3.55E-23 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth