YDL015C / TSC13

Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL015C / TSC13

Click on Significant Values for Screen Details ID:SGTC_169|Compound:k015-0017|FD-Score:-6.63|P-value:1.44E-11 ID:SGTC_170|Compound:4197-0004|FD-Score:4.66|P-value:1.58E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:3.52|P-value:2.19E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:3.30|P-value:4.94E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.82|P-value:6.87E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.43|P-value:3.08E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.56|P-value:1.91E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.68|P-value:1.08E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:-3.38|P-value:3.40E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.74|P-value:9.41E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:3.46|P-value:2.71E-4 ID:SGTC_917|Compound:harmaline|FD-Score:4.62|P-value:1.97E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.90|P-value:4.76E-7 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.13|P-value:8.28E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.62|P-value:1.52E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-3.21|P-value:6.30E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.55|P-value:1.93E-4 ID:SGTC_1629|Compound:st005085|FD-Score:5.10|P-value:1.73E-7 ID:SGTC_1632|Compound:st005650|FD-Score:5.85|P-value:2.38E-9 ID:SGTC_1673|Compound:st016611|FD-Score:4.73|P-value:1.14E-6 ID:SGTC_1741|Compound:st038134|FD-Score:3.21|P-value:6.81E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-3.25|P-value:5.33E-4 ID:SGTC_2058|Compound:5236698|FD-Score:5.12|P-value:1.51E-7 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.37|P-value:3.87E-4 ID:SGTC_2237|Compound:6667747|FD-Score:10.20|P-value:5.81E-25 ID:SGTC_2238|Compound:6671620|FD-Score:3.91|P-value:4.66E-5 ID:SGTC_2783|Compound:7738165|FD-Score:4.36|P-value:6.71E-6 ID:SGTC_2987|Compound:9018338|FD-Score:3.13|P-value:8.88E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:-6.63|P-value:1.44E-11 ID:SGTC_170|Compound:4197-0004|FD-Score:4.66|P-value:1.58E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:3.52|P-value:2.19E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:3.30|P-value:4.94E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.82|P-value:6.87E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.43|P-value:3.08E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.56|P-value:1.91E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.68|P-value:1.08E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:-3.38|P-value:3.40E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.74|P-value:9.41E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:3.46|P-value:2.71E-4 ID:SGTC_917|Compound:harmaline|FD-Score:4.62|P-value:1.97E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.90|P-value:4.76E-7 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.13|P-value:8.28E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.62|P-value:1.52E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:-3.21|P-value:6.30E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.55|P-value:1.93E-4 ID:SGTC_1629|Compound:st005085|FD-Score:5.10|P-value:1.73E-7 ID:SGTC_1632|Compound:st005650|FD-Score:5.85|P-value:2.38E-9 ID:SGTC_1673|Compound:st016611|FD-Score:4.73|P-value:1.14E-6 ID:SGTC_1741|Compound:st038134|FD-Score:3.21|P-value:6.81E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-3.25|P-value:5.33E-4 ID:SGTC_2058|Compound:5236698|FD-Score:5.12|P-value:1.51E-7 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.37|P-value:3.87E-4 ID:SGTC_2237|Compound:6667747|FD-Score:10.20|P-value:5.81E-25 ID:SGTC_2238|Compound:6671620|FD-Score:3.91|P-value:4.66E-5 ID:SGTC_2783|Compound:7738165|FD-Score:4.36|P-value:6.71E-6 ID:SGTC_2987|Compound:9018338|FD-Score:3.13|P-value:8.88E-4

Top fitness defect scores for YDL015C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2237 6667747 10.20 47.2 µM 5.81E-25 1 6.48E-25 4.04
2 SGTC_1632 st005650 5.85 90.0 µM 2.38E-9 1 2.39E-9 0.90
3 SGTC_2058 5236698 5.12 133.0 µM 1.51E-7 2 1.50E-7 1.23
4 SGTC_1629 st005085 5.10 44.9 µM 1.73E-7 RPP1 & pyrimidine depletion 9 1.72E-7 0.23
5 SGTC_1056 3448-1962 4.90 115.0 µM 4.76E-7 amide catabolism 2 4.72E-7 0.40
6 SGTC_1673 st016611 4.73 27.3 µM 1.14E-6 3 1.13E-6 0.38
7 SGTC_170 4197-0004 4.66 226.0 µM 1.58E-6 2 1.56E-6 0.64
8 SGTC_917 harmaline 4.62 728.1 nM 1.97E-6 ergosterol biosynthesis 5 1.95E-6 0.18
9 SGTC_2783 7738165 4.36 71.4 µM 6.71E-6 3 6.63E-6 0.38
10 SGTC_2238 6671620 3.91 73.2 µM 4.66E-5 5 4.59E-5 0.31
11 SGTC_225 nsc-122657 3.82 1.0 µM 6.87E-5 3 6.76E-5 0.18
12 SGTC_579 4491-1009 3.74 110.0 µM 9.41E-5 60S ribosome export 8 9.26E-5 0.10
13 SGTC_1159 4092-0538 3.62 61.2 µM 1.52E-4 9 1.50E-4 0.08
14 SGTC_362 0310-0100 3.56 9.0 µM 1.91E-4 RPP1 & pyrimidine depletion 12 1.88E-4 0.24
15 SGTC_1473 k844-0725 3.55 134.0 µM 1.93E-4 8 1.90E-4 0.06
16 SGTC_178 4092-0806 3.52 87.5 µM 2.19E-4 ergosterol depletion effects on membrane 28 2.15E-4 0.20
17 SGTC_708 3448-5400 3.46 146.0 µM 2.71E-4 30 2.66E-4 0.09
18 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.43 4.6 µM 3.08E-4 heat shock/prefoldin 27 3.03E-4 0.03
19 SGTC_2131 toluylene red 3.37 5.3 µM 3.87E-4 60S ribosome export 20 3.80E-4 0.12
20 SGTC_181 1447-1644 3.30 8.1 µM 4.94E-4 endomembrane recycling 17 4.85E-4 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 8.08E-13 YLR348C DIC1 hom Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
0.122 1.17E-12 YGL248W PDE1 hom Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
0.115 2.58E-11 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.108 3.15E-10 YOR168W GLN4 het Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
0.103 2.34E-9 YIL045W PIG2 hom Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase
0.102 2.80E-9 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.092 8.30E-8 YDL161W ENT1 hom Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.090 1.79E-7 YNL243W SLA2 hom Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo
0.088 3.01E-7 YJR050W ISY1 hom Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
0.087 3.90E-7 YGR063C SPT4 hom Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
0.087 4.58E-7 YGR168C_p YGR168C_p hom Putative protein of unknown function; YGR168C is not an essential gene
0.087 4.88E-7 YOL073C YOL073C hom Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
0.086 5.59E-7 YFR033C QCR6 hom Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
0.085 8.06E-7 YBR180W DTR1 hom Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation
0.085 9.24E-7 YDL133C-A RPL41B hom Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication