YDL017W / CDC7

DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL017W / CDC7

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:2.77|P-value:8.79E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.36|P-value:7.17E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:-3.12|P-value:1.98E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-2.98|P-value:3.69E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.17|P-value:1.66E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:2.75|P-value:9.40E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.05|P-value:2.81E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:-3.49|P-value:3.84E-5 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.25|P-value:1.17E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:2.98|P-value:3.68E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:2.92|P-value:4.73E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:4.00|P-value:3.03E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.36|P-value:7.06E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:2.87|P-value:5.75E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.86|P-value:6.31E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.20|P-value:1.41E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.23|P-value:1.27E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:2.95|P-value:4.30E-4 ID:SGTC_1429|Compound:4049-0205|FD-Score:-3.37|P-value:6.64E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:-3.67|P-value:1.60E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.27|P-value:1.06E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-2.92|P-value:4.74E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.24|P-value:1.21E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-2.80|P-value:7.54E-4 ID:SGTC_2113|Compound:5491891|FD-Score:-2.74|P-value:9.53E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.04|P-value:2.90E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.53|P-value:3.25E-5 ID:SGTC_2283|Compound:7944793|FD-Score:-3.77|P-value:9.59E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-2.94|P-value:4.26E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.63|P-value:1.95E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-2.86|P-value:6.04E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.23|P-value:1.26E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.25|P-value:1.17E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.97|P-value:3.92E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.02|P-value:3.21E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:2.77|P-value:8.79E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.36|P-value:7.17E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:-3.12|P-value:1.98E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-2.98|P-value:3.69E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.17|P-value:1.66E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:2.75|P-value:9.40E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.05|P-value:2.81E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:-3.49|P-value:3.84E-5 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.25|P-value:1.17E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:2.98|P-value:3.68E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:2.92|P-value:4.73E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:4.00|P-value:3.03E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.36|P-value:7.06E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:2.87|P-value:5.75E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.86|P-value:6.31E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.20|P-value:1.41E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.23|P-value:1.27E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:2.95|P-value:4.30E-4 ID:SGTC_1429|Compound:4049-0205|FD-Score:-3.37|P-value:6.64E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:-3.67|P-value:1.60E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.27|P-value:1.06E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-2.92|P-value:4.74E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.24|P-value:1.21E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-2.80|P-value:7.54E-4 ID:SGTC_2113|Compound:5491891|FD-Score:-2.74|P-value:9.53E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.04|P-value:2.90E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.53|P-value:3.25E-5 ID:SGTC_2283|Compound:7944793|FD-Score:-3.77|P-value:9.59E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-2.94|P-value:4.26E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.63|P-value:1.95E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-2.86|P-value:6.04E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.23|P-value:1.26E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.25|P-value:1.17E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.97|P-value:3.92E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.02|P-value:3.21E-4

Top fitness defect scores for YDL017W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1047 2043-3044 4.00 82.0 µM 3.03E-6 7 3.20E-5 0.01
2 SGTC_1158 4092-0494 3.86 91.8 µM 6.31E-6 5 5.71E-5 0.05
3 SGTC_2412 st077135 3.63 33.4 µM 1.95E-5 7 1.39E-4 0.06
4 SGTC_2183 6420625 3.53 130.5 µM 3.25E-5 mitochondrial processes 21 2.09E-4 0.02
5 SGTC_1118 chelidonine 3.36 707.5 nM 7.06E-5 6 3.85E-4 0.03
6 SGTC_152 4469-0003 3.36 551.2 µM 7.17E-5 23 3.90E-4 3.97E-5
7 SGTC_2693 st077581 3.25 40.7 µM 1.17E-4 9 5.75E-4 0.00
8 SGTC_2605 digitonin 3.23 300.0 nM 1.26E-4 10 6.09E-4 0.05
9 SGTC_1371 2421-0008 3.23 32.7 µM 1.27E-4 mitochondrial processes 34 6.14E-4 0.04
10 SGTC_649 1123-0311 3.17 367.0 µM 1.66E-4 endomembrane recycling 19 7.57E-4 0.07
11 SGTC_790 1575-0012 3.05 79.1 µM 2.81E-4 TSC3-RPN4 13 0.00115 0.04
12 SGTC_2167 5876490 3.04 200.0 µM 2.90E-4 7 0.00118 0.17
13 SGTC_2851 9015922 3.02 71.4 µM 3.21E-4 14 0.00128 0.01
14 SGTC_902 1120-0002 2.98 2.1 µM 3.68E-4 azole & statin 27 0.00143 0.00
15 SGTC_2764 methyldopa 2.97 94.7 µM 3.92E-4 13 0.00150 0.07
16 SGTC_1381 2324-0066 2.95 62.1 µM 4.30E-4 21 0.00161 0.09
17 SGTC_977 1348-1378 2.92 135.0 µM 4.73E-4 21 0.00174 0.08
18 SGTC_1157 4092-0394 2.87 27.6 µM 5.75E-4 15 0.00203 0.02
19 SGTC_20 0481-0013 2.77 4.9 µM 8.79E-4 29 0.00285 0.01
20 SGTC_722 3534-0461 2.75 190.0 µM 9.40E-4 PDR1 10 0.00300 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 5.80E-12 YOR149C SMP3 het Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
0.116 1.92E-11 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.104 1.33E-9 YOR020W-A_p YOR020W-A_p hom Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.104 1.64E-9 YMR213W CEF1 het Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
0.103 2.51E-9 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.094 4.36E-8 YJR023C_d YJR023C_d het Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
0.088 3.04E-7 YBR125C PTC4 hom Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
0.087 3.89E-7 YLR216C CPR6 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
0.085 7.23E-7 YFR032C-A RPL29 hom Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
0.085 9.14E-7 YGR094W VAS1 het Mitochondrial and cytoplasmic valyl-tRNA synthetase
0.084 1.17E-6 YHR071W PCL5 hom Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
0.082 1.89E-6 YOR175C ALE1 hom Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
0.080 3.34E-6 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.080 3.72E-6 YDR397C NCB2 het Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
0.079 4.96E-6 YHR153C SPO16 hom Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation