YDL025C / RTK1

Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL025C / RTK1

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-5.11|P-value:3.62E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:12.70|P-value:2.62E-28 ID:SGTC_145|Compound:3737-0113|FD-Score:4.31|P-value:1.04E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.87|P-value:2.00E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.76|P-value:3.28E-7 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.60|P-value:7.56E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:4.01|P-value:2.82E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.03|P-value:2.60E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.26|P-value:2.04E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:7.14|P-value:3.33E-10 ID:SGTC_601|Compound:0986-0029|FD-Score:-3.70|P-value:5.69E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.93|P-value:7.66E-6 ID:SGTC_638|Compound:3970-0837|FD-Score:5.76|P-value:3.29E-7 ID:SGTC_649|Compound:1123-0311|FD-Score:4.68|P-value:2.73E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:5.18|P-value:3.88E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:5.15|P-value:4.50E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.56|P-value:8.44E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-5.45|P-value:8.64E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:4.13|P-value:1.89E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.58|P-value:5.58E-14 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.98|P-value:3.05E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.67|P-value:7.95E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.26|P-value:1.23E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.66|P-value:8.15E-4 ID:SGTC_1625|Compound:st003711|FD-Score:-3.74|P-value:4.93E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-5.42|P-value:1.01E-6 ID:SGTC_1630|Compound:st005209|FD-Score:-4.74|P-value:1.59E-5 ID:SGTC_1783|Compound:st039459|FD-Score:3.78|P-value:5.77E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-4.03|P-value:1.97E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.36|P-value:6.30E-5 ID:SGTC_2051|Compound:5263410|FD-Score:5.01|P-value:7.69E-6 ID:SGTC_2081|Compound:5214970|FD-Score:5.54|P-value:8.49E-7 ID:SGTC_2293|Compound:7624732|FD-Score:4.65|P-value:3.07E-5 ID:SGTC_2368|Compound:9071271|FD-Score:3.94|P-value:3.52E-4 ID:SGTC_2440|Compound:5628896|FD-Score:4.15|P-value:1.77E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.55|P-value:8.83E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.93|P-value:1.06E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.02|P-value:2.67E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.70|P-value:7.42E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.84|P-value:4.79E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.78|P-value:1.34E-5 ID:SGTC_3145|Compound:9085516|FD-Score:3.63|P-value:9.11E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.62|P-value:9.22E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-5.11|P-value:3.62E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:12.70|P-value:2.62E-28 ID:SGTC_145|Compound:3737-0113|FD-Score:4.31|P-value:1.04E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.87|P-value:2.00E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.76|P-value:3.28E-7 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.60|P-value:7.56E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:4.01|P-value:2.82E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.03|P-value:2.60E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.26|P-value:2.04E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:7.14|P-value:3.33E-10 ID:SGTC_601|Compound:0986-0029|FD-Score:-3.70|P-value:5.69E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.93|P-value:7.66E-6 ID:SGTC_638|Compound:3970-0837|FD-Score:5.76|P-value:3.29E-7 ID:SGTC_649|Compound:1123-0311|FD-Score:4.68|P-value:2.73E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:5.18|P-value:3.88E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:5.15|P-value:4.50E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.56|P-value:8.44E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-5.45|P-value:8.64E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:4.13|P-value:1.89E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.58|P-value:5.58E-14 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.98|P-value:3.05E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.67|P-value:7.95E-4 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.26|P-value:1.23E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.66|P-value:8.15E-4 ID:SGTC_1625|Compound:st003711|FD-Score:-3.74|P-value:4.93E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-5.42|P-value:1.01E-6 ID:SGTC_1630|Compound:st005209|FD-Score:-4.74|P-value:1.59E-5 ID:SGTC_1783|Compound:st039459|FD-Score:3.78|P-value:5.77E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-4.03|P-value:1.97E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.36|P-value:6.30E-5 ID:SGTC_2051|Compound:5263410|FD-Score:5.01|P-value:7.69E-6 ID:SGTC_2081|Compound:5214970|FD-Score:5.54|P-value:8.49E-7 ID:SGTC_2293|Compound:7624732|FD-Score:4.65|P-value:3.07E-5 ID:SGTC_2368|Compound:9071271|FD-Score:3.94|P-value:3.52E-4 ID:SGTC_2440|Compound:5628896|FD-Score:4.15|P-value:1.77E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.55|P-value:8.83E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.93|P-value:1.06E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.02|P-value:2.67E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.70|P-value:7.42E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.84|P-value:4.79E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.78|P-value:1.34E-5 ID:SGTC_3145|Compound:9085516|FD-Score:3.63|P-value:9.11E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.62|P-value:9.22E-4

Top fitness defect scores for YDL025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 12.70 9.0 µM 2.62E-28 cell wall 42 5.43E-37
2 SGTC_1161 k007-0610 8.58 110.0 µM 5.58E-14 ERAD & cell cycle 21 4.82E-18
3 SGTC_457 grayanotoxin iii 7.14 135.0 µM 3.33E-10 14 4.71E-13
4 SGTC_146 3448-8650 5.87 53.1 µM 2.00E-7 NEO1-PIK1 57 2.19E-9
5 SGTC_221 trifluoperazine 5.76 10.3 µM 3.28E-7 NEO1-PIK1 128 4.20E-9
6 SGTC_638 3970-0837 5.76 4.1 µM 3.29E-7 14 4.23E-9
7 SGTC_2081 5214970 5.54 32.3 µM 8.49E-7 4 1.47E-8
8 SGTC_795 0685-0265 5.18 78.0 µM 3.88E-6 excess fatty acid 10 1.09E-7
9 SGTC_881 0833-0569 5.15 170.0 µM 4.50E-6 4 1.33E-7
10 SGTC_2051 5263410 5.01 176.0 µM 7.69E-6 9 2.69E-7
11 SGTC_2596 sinensetin 4.93 100.0 µM 1.06E-5 13 4.11E-7
12 SGTC_649 1123-0311 4.68 367.0 µM 2.73E-5 endomembrane recycling 43 1.43E-6
13 SGTC_2293 7624732 4.65 200.0 µM 3.07E-5 13 1.66E-6
14 SGTC_145 3737-0113 4.31 38.4 µM 1.04E-4 DNA intercalators 58 8.25E-6
15 SGTC_1559 coumarin 343 4.26 70.1 µM 1.23E-4 10 1.04E-5
16 SGTC_2440 5628896 4.15 157.8 µM 1.77E-4 23 1.66E-5
17 SGTC_1159 4092-0538 4.13 61.2 µM 1.89E-4 25 1.82E-5
18 SGTC_304 1310-0298 4.03 97.0 µM 2.60E-4 cell wall 59 2.77E-5
19 SGTC_2806 7952793 4.02 58.4 µM 2.67E-4 19 2.87E-5
20 SGTC_288 1315-0019 4.01 35.1 µM 2.82E-4 RSC & ERG11 22 3.08E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.269 1.67E-56 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.244 1.10E-46 YNL115C_p YNL115C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
0.229 2.56E-41 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.228 7.44E-41 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.227 2.08E-40 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.225 7.94E-40 YBR296C PHO89 hom Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
0.223 3.99E-39 YNL077W APJ1 hom Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
0.209 2.66E-34 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.204 8.38E-33 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.201 8.57E-32 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.198 6.44E-31 YNL211C_p YNL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
0.196 1.91E-30 YNR044W AGA1 hom Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
0.196 1.98E-30 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.194 8.72E-30 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.194 9.14E-30 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p