YDL028C / MPS1

Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL028C / MPS1

Click on Significant Values for Screen Details ID:SGTC_149|Compound:3970-0822|FD-Score:3.52|P-value:4.09E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-3.47|P-value:4.11E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.67|P-value:2.03E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-3.30|P-value:7.26E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.45|P-value:5.17E-4 ID:SGTC_1608|Compound:st001473|FD-Score:4.00|P-value:7.10E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.39|P-value:6.36E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.21|P-value:9.76E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.49|P-value:3.91E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.52|P-value:4.09E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-3.47|P-value:4.11E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.67|P-value:2.03E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-3.30|P-value:7.26E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.45|P-value:5.17E-4 ID:SGTC_1608|Compound:st001473|FD-Score:4.00|P-value:7.10E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.39|P-value:6.36E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.21|P-value:9.76E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.49|P-value:3.91E-4

Top fitness defect scores for YDL028C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1608 st001473 4.00 40.5 µM 7.10E-5 5 3.18E-5 0.14
2 SGTC_149 3970-0822 3.52 46.1 µM 4.09E-4 tubulin folding & SWR complex 18 2.16E-4 0.04
3 SGTC_1317 1273-0028 3.45 910.0 nM 5.17E-4 azole & statin 18 2.79E-4 0.03
4 SGTC_2485 5633444 3.39 17.8 µM 6.36E-4 redox potentiating 12 3.49E-4 0.02
5 SGTC_6 0844-0013 3.10 19.8 µM 0.00159 25 9.52E-4 0.03
6 SGTC_860 0443-0016 3.10 25.1 µM 0.00161 16 9.60E-4 0.10
7 SGTC_2005 4024620 3.09 188.0 µM 0.00165 10 9.90E-4 0.08
8 SGTC_1549 4'-methoxyflavonol 3.02 74.6 µM 0.00208 5 0.00127 0.23
9 SGTC_1864 st057645 2.96 30.4 µM 0.00246 amide catabolism 11 0.00153 0.07
10 SGTC_2587 piperic acid 2.93 92.2 µM 0.00269 19 0.00169 0.09
11 SGTC_1017 4239-0030 2.92 168.0 µM 0.00278 8 0.00175 0.10
12 SGTC_1863 st057641 2.91 20.1 µM 0.00288 17 0.00182 0.02
13 SGTC_470 aa-861 2.86 153.0 µM 0.00327 sphingolipid biosynthesis & PDR1 30 0.00209 0.02
14 SGTC_749 1000-0129 2.84 128.0 µM 0.00356 amide catabolism 12 0.00229 0.08
15 SGTC_3204 9114183 2.81 49.5 µM 0.00382 22 0.00247 0.02
16 SGTC_1536 cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid 2.79 61.9 µM 0.00411 14 0.00267 0.09
17 SGTC_2580 diallyl disulphide 2.78 81.4 µM 0.00418 26 0.00273 0.18
18 SGTC_433 pimozide 2.78 108.0 µM 0.00421 6 0.00275 0.02
19 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 2.75 54.9 µM 0.00458 redox potentiating 19 0.00301 0.00
20 SGTC_2026 5148700 2.74 152.0 µM 0.00460 17 0.00303 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.136 2.34E-15 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.130 3.63E-14 YCL034W LSB5 hom Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat
0.125 3.68E-13 YBR265W TSC10 het 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.117 1.11E-11 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.117 1.29E-11 YOR220W RCN2 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
0.111 1.04E-10 YML081W TDA9 hom DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
0.108 4.03E-10 YML035C AMD1 hom AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
0.108 4.02E-10 YBR228W SLX1 hom Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
0.102 2.90E-9 YPR071W_p YPR071W_p hom Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C
0.096 2.75E-8 YFL030W AGX1 hom Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases
0.094 5.69E-8 YLR366W_d YLR366W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
0.087 4.73E-7 YGL167C PMR1 hom High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
0.086 5.41E-7 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.085 8.81E-7 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.085 8.97E-7 YPL186C UIP4 hom Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope