YDL029W / ARP2

Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL029W / ARP2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:7.59|P-value:1.60E-12 ID:SGTC_382|Compound:3229-0966|FD-Score:4.82|P-value:4.93E-6 ID:SGTC_596|Compound:1155-0152|FD-Score:3.87|P-value:1.99E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.46|P-value:7.81E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.39|P-value:8.37E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.44|P-value:7.04E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.31|P-value:3.25E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.52|P-value:6.32E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.62|P-value:4.56E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.80|P-value:4.45E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.62|P-value:3.92E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:9.15|P-value:2.09E-17 ID:SGTC_1523|Compound:st012844|FD-Score:-7.69|P-value:5.81E-13 ID:SGTC_1529|Compound:apigenin|FD-Score:-4.14|P-value:6.16E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.87|P-value:1.95E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.59|P-value:5.13E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.46|P-value:7.80E-4 ID:SGTC_1725|Compound:st036284|FD-Score:5.42|P-value:3.28E-7 ID:SGTC_1735|Compound:st034310|FD-Score:4.75|P-value:6.58E-6 ID:SGTC_1736|Compound:st036774|FD-Score:5.02|P-value:2.06E-6 ID:SGTC_1739|Compound:st037196|FD-Score:-4.26|P-value:3.95E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.66|P-value:4.10E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.52|P-value:6.47E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.70|P-value:3.53E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.59|P-value:4.33E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.73|P-value:2.73E-4 ID:SGTC_2160|Compound:5660466|FD-Score:6.00|P-value:1.84E-8 ID:SGTC_2177|Compound:5811945|FD-Score:-3.46|P-value:6.68E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.69|P-value:3.67E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.78|P-value:2.31E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.50|P-value:5.78E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.44|P-value:8.15E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.78|P-value:5.84E-6 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.58|P-value:5.19E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.40|P-value:9.47E-4 ID:SGTC_2904|Compound:7225135|FD-Score:4.27|P-value:4.64E-5 ID:SGTC_2944|Compound:9067346|FD-Score:3.67|P-value:3.88E-4 ID:SGTC_3143|Compound:9086658|FD-Score:4.06|P-value:1.00E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.46|P-value:7.75E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:7.59|P-value:1.60E-12 ID:SGTC_382|Compound:3229-0966|FD-Score:4.82|P-value:4.93E-6 ID:SGTC_596|Compound:1155-0152|FD-Score:3.87|P-value:1.99E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.46|P-value:7.81E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.39|P-value:8.37E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.44|P-value:7.04E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.31|P-value:3.25E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.52|P-value:6.32E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.62|P-value:4.56E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.80|P-value:4.45E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.62|P-value:3.92E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:9.15|P-value:2.09E-17 ID:SGTC_1523|Compound:st012844|FD-Score:-7.69|P-value:5.81E-13 ID:SGTC_1529|Compound:apigenin|FD-Score:-4.14|P-value:6.16E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.87|P-value:1.95E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.59|P-value:5.13E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.46|P-value:7.80E-4 ID:SGTC_1725|Compound:st036284|FD-Score:5.42|P-value:3.28E-7 ID:SGTC_1735|Compound:st034310|FD-Score:4.75|P-value:6.58E-6 ID:SGTC_1736|Compound:st036774|FD-Score:5.02|P-value:2.06E-6 ID:SGTC_1739|Compound:st037196|FD-Score:-4.26|P-value:3.95E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.66|P-value:4.10E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.52|P-value:6.47E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.70|P-value:3.53E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.59|P-value:4.33E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.73|P-value:2.73E-4 ID:SGTC_2160|Compound:5660466|FD-Score:6.00|P-value:1.84E-8 ID:SGTC_2177|Compound:5811945|FD-Score:-3.46|P-value:6.68E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.69|P-value:3.67E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.78|P-value:2.31E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.50|P-value:5.78E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.44|P-value:8.15E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.78|P-value:5.84E-6 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.58|P-value:5.19E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.40|P-value:9.47E-4 ID:SGTC_2904|Compound:7225135|FD-Score:4.27|P-value:4.64E-5 ID:SGTC_2944|Compound:9067346|FD-Score:3.67|P-value:3.88E-4 ID:SGTC_3143|Compound:9086658|FD-Score:4.06|P-value:1.00E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.46|P-value:7.75E-4

Top fitness defect scores for YDL029W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1512 0259-0544 9.15 12.2 µM 2.09E-17 1 2.84E-20 3.61
2 SGTC_6 0844-0013 7.59 19.8 µM 1.60E-12 1 1.63E-14 2.55
3 SGTC_2160 5660466 6.00 132.9 µM 1.84E-8 60S ribosome export 4 9.88E-10 0.40
4 SGTC_1725 st036284 5.42 20.6 µM 3.28E-7 2 2.93E-8 0.91
5 SGTC_1736 st036774 5.02 51.0 µM 2.06E-6 1 2.55E-7 1.12
6 SGTC_382 3229-0966 4.82 23.2 µM 4.93E-6 2 7.12E-7 0.18
7 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.78 3.7 µM 5.84E-6 7 8.70E-7 0.01
8 SGTC_1735 st034310 4.75 56.8 µM 6.58E-6 Golgi 2 1.00E-6 0.10
9 SGTC_2904 7225135 4.27 42.0 µM 4.64E-5 1 9.93E-6 0.74
10 SGTC_3143 9086658 4.06 49.5 µM 1.00E-4 12 2.45E-5 0.01
11 SGTC_1587 estradiol valerate 3.87 56.1 µM 1.95E-4 2 5.35E-5 0.01
12 SGTC_596 1155-0152 3.87 116.0 µM 1.99E-4 TRP & mitochondrial translation 7 5.50E-5 0.01
13 SGTC_2005 4024620 3.70 188.0 µM 3.53E-4 5 1.08E-4 0.02
14 SGTC_2304 7443016 3.69 91.4 µM 3.67E-4 6 1.13E-4 0.08
15 SGTC_2944 9067346 3.67 25.6 µM 3.88E-4 15 1.20E-4 0.06
16 SGTC_1856 st057054 3.66 55.7 µM 4.10E-4 4 1.28E-4 0.50
17 SGTC_1405 3909-7894 3.62 12.0 µM 4.56E-4 60S ribosome export 2 1.45E-4 0.02
18 SGTC_1624 st003710 3.59 6.2 µM 5.13E-4 calcium & mitochondrial duress 4 1.67E-4 0.57
19 SGTC_2672 12a-hydroxy-5-deoxydehydromunduserone 3.58 100.0 µM 5.19E-4 1 1.69E-4 0.47
20 SGTC_1189 1431-2119 3.52 7.3 µM 6.32E-4 13 2.13E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 1.30E-24 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.149 3.33E-18 YBL064C PRX1 hom Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
0.149 3.85E-18 YLR314C CDC3 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.145 3.63E-17 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.143 9.41E-17 YFL046W_p FMP32_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.133 9.89E-15 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.131 3.12E-14 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.129 5.52E-14 YDR085C AFR1 hom Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.126 2.06E-13 YMR191W SPG5 hom Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.126 2.29E-13 YLR421C RPN13 hom Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
0.126 2.39E-13 YLR366W_d YLR366W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
0.125 3.64E-13 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.124 5.18E-13 YPL071C_p YPL071C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.123 8.10E-13 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.123 1.04E-12 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities