YDL031W / DBP10

Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL031W / DBP10

Click on Significant Values for Screen Details ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.19|P-value:4.81E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.08|P-value:7.22E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.64|P-value:8.39E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.50|P-value:1.47E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.45|P-value:1.76E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:4.60|P-value:9.89E-7 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.95|P-value:2.22E-5 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.35|P-value:2.62E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.42|P-value:2.01E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.49|P-value:1.52E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:5.53|P-value:5.19E-9 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.92|P-value:2.55E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.01|P-value:9.35E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:4.09|P-value:1.16E-5 ID:SGTC_1753|Compound:st043925|FD-Score:4.01|P-value:1.66E-5 ID:SGTC_1765|Compound:st044815|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.37|P-value:2.47E-4 ID:SGTC_1962|Compound:st076595|FD-Score:-3.18|P-value:5.05E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.95|P-value:1.51E-7 ID:SGTC_2058|Compound:5236698|FD-Score:3.12|P-value:6.22E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2152|Compound:5568786|FD-Score:3.18|P-value:5.09E-4 ID:SGTC_2281|Compound:7943571|FD-Score:3.33|P-value:2.81E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.89|P-value:2.91E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.34|P-value:2.72E-4 ID:SGTC_2443|Compound:5493264|FD-Score:-3.08|P-value:7.17E-4 ID:SGTC_2465|Compound:5809242|FD-Score:3.11|P-value:6.55E-4 ID:SGTC_2473|Compound:5854593|FD-Score:-3.32|P-value:3.00E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:3.08|P-value:7.30E-4 ID:SGTC_2735|Compound:sertaconazole|FD-Score:3.78|P-value:4.55E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.08|P-value:7.27E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.17|P-value:5.11E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.61|P-value:9.43E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.98|P-value:1.25E-7 ID:SGTC_3201|Compound:9113999|FD-Score:5.47|P-value:7.54E-9 ID:SGTC_3292|Compound:9120825|FD-Score:3.00|P-value:9.39E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.19|P-value:4.81E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.08|P-value:7.22E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.64|P-value:8.39E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.50|P-value:1.47E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.45|P-value:1.76E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:4.60|P-value:9.89E-7 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.95|P-value:2.22E-5 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.35|P-value:2.62E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.42|P-value:2.01E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.49|P-value:1.52E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:5.53|P-value:5.19E-9 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.92|P-value:2.55E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.01|P-value:9.35E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:4.09|P-value:1.16E-5 ID:SGTC_1753|Compound:st043925|FD-Score:4.01|P-value:1.66E-5 ID:SGTC_1765|Compound:st044815|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.37|P-value:2.47E-4 ID:SGTC_1962|Compound:st076595|FD-Score:-3.18|P-value:5.05E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.95|P-value:1.51E-7 ID:SGTC_2058|Compound:5236698|FD-Score:3.12|P-value:6.22E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2152|Compound:5568786|FD-Score:3.18|P-value:5.09E-4 ID:SGTC_2281|Compound:7943571|FD-Score:3.33|P-value:2.81E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.89|P-value:2.91E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.34|P-value:2.72E-4 ID:SGTC_2443|Compound:5493264|FD-Score:-3.08|P-value:7.17E-4 ID:SGTC_2465|Compound:5809242|FD-Score:3.11|P-value:6.55E-4 ID:SGTC_2473|Compound:5854593|FD-Score:-3.32|P-value:3.00E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:3.08|P-value:7.30E-4 ID:SGTC_2735|Compound:sertaconazole|FD-Score:3.78|P-value:4.55E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.08|P-value:7.27E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.17|P-value:5.11E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.61|P-value:9.43E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.98|P-value:1.25E-7 ID:SGTC_3201|Compound:9113999|FD-Score:5.47|P-value:7.54E-9 ID:SGTC_3292|Compound:9120825|FD-Score:3.00|P-value:9.39E-4

Top fitness defect scores for YDL031W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1111 tnp00062 5.53 510.7 nM 5.19E-9 3 1.57E-8 1.54
2 SGTC_3201 9113999 5.47 49.5 µM 7.54E-9 1 2.23E-8 0.44
3 SGTC_2019 4016004 4.95 108.0 µM 1.51E-7 5 3.69E-7 0.16
4 SGTC_999 4022-0066 4.60 22.7 µM 9.89E-7 Golgi 6 2.15E-6 0.01
5 SGTC_1238 0333-0287 4.09 48.8 µM 1.16E-5 Golgi 5 2.16E-5 0.49
6 SGTC_1753 st043925 4.01 64.0 µM 1.66E-5 3 3.02E-5 0.54
7 SGTC_1218 1348-1416 3.92 30.7 µM 2.55E-5 SWF1 & branched chain AA biosynthesis 3 4.51E-5 0.13
8 SGTC_2404 5161160 3.89 200.0 µM 2.91E-5 8 5.11E-5 0.03
9 SGTC_2735 sertaconazole 3.78 140.0 nM 4.55E-5 plasma membrane duress 3 7.76E-5 0.01
10 SGTC_3032 9089427 3.61 49.5 µM 9.43E-5 60S ribosome export 6 1.54E-4 0.11
11 SGTC_464 bw-b 70c 3.50 158.0 µM 1.47E-4 11 2.33E-4 0.40
12 SGTC_752 1896-0090 3.45 130.0 µM 1.76E-4 TSC3-RPN4 10 2.76E-4 0.20
13 SGTC_1014 4130-3005 3.42 69.3 µM 2.01E-4 10 3.13E-4 0.26
14 SGTC_1865 st057639 3.37 52.2 µM 2.47E-4 6 3.80E-4 0.20
15 SGTC_2100 5377183 3.36 190.0 µM 2.52E-4 60S ribosome export 14 3.87E-4 0.02
16 SGTC_2419 paclitaxel 3.34 166.5 µM 2.72E-4 10 4.15E-4 0.02
17 SGTC_2281 7943571 3.33 200.0 µM 2.81E-4 11 4.28E-4 0.08
18 SGTC_120 rhodamine 6G 3.19 52.3 µM 4.81E-4 mitochondrial processes 33 7.09E-4 2.27E-4
19 SGTC_2152 5568786 3.18 200.0 µM 5.09E-4 11 7.48E-4 0.11
20 SGTC_3006 9075424 3.17 71.4 µM 5.11E-4 14 7.50E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.81E-18 YDR170C SEC7 het Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
0.134 6.11E-15 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.133 8.72E-15 YHL016C DUR3 hom Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.130 3.33E-14 YGL165C_d YGL165C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
0.128 1.00E-13 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.120 3.15E-12 YIL099W SGA1 hom Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation
0.112 8.94E-11 YGL111W NSA1 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
0.110 1.87E-10 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.106 7.62E-10 YBR084W MIS1 hom Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
0.102 2.96E-9 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.100 6.84E-9 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.098 1.17E-8 YLR314C CDC3 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.096 2.52E-8 YLR105C SEN2 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
0.095 3.18E-8 YGR248W SOL4 hom 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
0.093 6.77E-8 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease