YDL045C / FAD1

Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL045C / FAD1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:15.20|P-value:6.21E-60 ID:SGTC_349|Compound:k015-0014|FD-Score:3.97|P-value:1.05E-5 ID:SGTC_350|Compound:k064-0020|FD-Score:3.76|P-value:2.71E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:2.98|P-value:6.99E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:3.69|P-value:3.83E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:3.10|P-value:4.32E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.11|P-value:4.22E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.76|P-value:1.63E-7 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:4.30|P-value:1.94E-6 ID:SGTC_1728|Compound:st035614|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.63|P-value:4.99E-5 ID:SGTC_1832|Compound:st054113|FD-Score:-3.34|P-value:1.74E-4 ID:SGTC_1835|Compound:st008666|FD-Score:-3.86|P-value:1.75E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.06|P-value:6.62E-6 ID:SGTC_1957|Compound:st077035|FD-Score:2.94|P-value:8.05E-4 ID:SGTC_2018|Compound:5133068|FD-Score:7.16|P-value:8.19E-15 ID:SGTC_2060|Compound:5238662|FD-Score:-3.62|P-value:5.38E-5 ID:SGTC_2157|Compound:5649910|FD-Score:2.96|P-value:7.53E-4 ID:SGTC_2404|Compound:5161160|FD-Score:2.97|P-value:7.08E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.51|P-value:8.35E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:-3.19|P-value:3.22E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-4.08|P-value:6.21E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:6.08|P-value:3.54E-11 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.21|P-value:2.85E-4 ID:SGTC_2904|Compound:7225135|FD-Score:3.08|P-value:4.82E-4 ID:SGTC_2979|Compound:9012552|FD-Score:2.89|P-value:9.77E-4 ID:SGTC_3023|Compound:9089636|FD-Score:3.17|P-value:3.29E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.19|P-value:3.14E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:15.20|P-value:6.21E-60 ID:SGTC_349|Compound:k015-0014|FD-Score:3.97|P-value:1.05E-5 ID:SGTC_350|Compound:k064-0020|FD-Score:3.76|P-value:2.71E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:2.98|P-value:6.99E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:3.69|P-value:3.83E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:3.10|P-value:4.32E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.11|P-value:4.22E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.76|P-value:1.63E-7 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:4.30|P-value:1.94E-6 ID:SGTC_1728|Compound:st035614|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.63|P-value:4.99E-5 ID:SGTC_1832|Compound:st054113|FD-Score:-3.34|P-value:1.74E-4 ID:SGTC_1835|Compound:st008666|FD-Score:-3.86|P-value:1.75E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.06|P-value:6.62E-6 ID:SGTC_1957|Compound:st077035|FD-Score:2.94|P-value:8.05E-4 ID:SGTC_2018|Compound:5133068|FD-Score:7.16|P-value:8.19E-15 ID:SGTC_2060|Compound:5238662|FD-Score:-3.62|P-value:5.38E-5 ID:SGTC_2157|Compound:5649910|FD-Score:2.96|P-value:7.53E-4 ID:SGTC_2404|Compound:5161160|FD-Score:2.97|P-value:7.08E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.51|P-value:8.35E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:-3.19|P-value:3.22E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-4.08|P-value:6.21E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:6.08|P-value:3.54E-11 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.21|P-value:2.85E-4 ID:SGTC_2904|Compound:7225135|FD-Score:3.08|P-value:4.82E-4 ID:SGTC_2979|Compound:9012552|FD-Score:2.89|P-value:9.77E-4 ID:SGTC_3023|Compound:9089636|FD-Score:3.17|P-value:3.29E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.19|P-value:3.14E-4

Top fitness defect scores for YDL045C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_16 erodoxin 15.20 22.8 µM 6.21E-60 unfolded protein response 2 1.99E-52 3.63
2 SGTC_2018 5133068 7.16 143.0 µM 8.19E-15 2 4.06E-13 0.95
3 SGTC_2621 lithochol-11-enic acid 6.08 100.0 µM 3.54E-11 1 6.04E-10 0.91
4 SGTC_1239 0337-0584 4.76 35.4 µM 1.63E-7 unfolded protein response 23 9.59E-7 0.33
5 SGTC_1292 0kpi-0043 4.30 232.0 µM 1.94E-6 1 8.36E-6 0.45
6 SGTC_1842 st055362 4.06 61.2 µM 6.62E-6 3 2.44E-5 0.30
7 SGTC_349 k015-0014 3.97 24.0 µM 1.05E-5 6 3.66E-5 0.02
8 SGTC_350 k064-0020 3.76 19.8 µM 2.71E-5 heme requiring 6 8.40E-5 0.05
9 SGTC_906 1165-0455 3.69 526.0 µM 3.83E-5 4 1.14E-4 0.22
10 SGTC_2517 totarol 3.51 10.0 µM 8.35E-5 7 2.25E-4 0.13
11 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 3.21 100.0 µM 2.85E-4 9 6.61E-4 0.13
12 SGTC_3203 9113543 3.19 49.5 µM 3.14E-4 18 7.19E-4 0.02
13 SGTC_3023 9089636 3.17 49.5 µM 3.29E-4 5 7.50E-4 0.07
14 SGTC_972 1319-0219 3.11 195.0 µM 4.22E-4 18 9.32E-4 0.05
15 SGTC_924 2046-0044 3.10 21.3 µM 4.32E-4 16 9.52E-4 0.05
16 SGTC_1728 st035614 3.08 33.8 µM 4.75E-4 15 0.00103 0.04
17 SGTC_2904 7225135 3.08 42.0 µM 4.82E-4 10 0.00105 0.02
18 SGTC_352 k048-0007 2.98 5.1 µM 6.99E-4 ERAD & cell cycle 26 0.00145 0.04
19 SGTC_2404 5161160 2.97 200.0 µM 7.08E-4 21 0.00147 0.13
20 SGTC_2157 5649910 2.96 96.4 µM 7.53E-4 18 0.00155 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.530 4.28E-242 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.430 4.67E-151 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.324 1.16E-82 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.321 4.59E-81 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.270 2.71E-57 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.264 1.15E-54 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.239 1.30E-44 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.219 1.05E-37 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.219 1.14E-37 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.200 1.66E-31 YCL052C PBN1 het Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
0.182 1.87E-26 YPR111W DBF20 hom Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs
0.169 5.22E-23 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.169 6.20E-23 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.152 8.47E-19 YCL051W LRE1 hom Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
0.145 3.20E-17 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization