YDL046W / NPC2

Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL046W / NPC2

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-3.49|P-value:2.26E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.56|P-value:1.84E-6 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.18|P-value:6.90E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.90|P-value:3.80E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:4.84|P-value:4.61E-7 ID:SGTC_709|Compound:3807-1509|FD-Score:7.88|P-value:7.28E-16 ID:SGTC_736|Compound:0958-0254|FD-Score:-3.23|P-value:5.79E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-4.21|P-value:1.12E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:4.22|P-value:9.15E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:4.34|P-value:5.27E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.41|P-value:3.07E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.14|P-value:7.05E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.53|P-value:1.91E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.59|P-value:1.56E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.82|P-value:6.16E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.26|P-value:5.24E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.15|P-value:6.90E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.72|P-value:7.84E-5 ID:SGTC_1387|Compound:3342-1166|FD-Score:-3.40|P-value:3.11E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.24|P-value:5.68E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.86|P-value:4.50E-5 ID:SGTC_1913|Compound:st060213|FD-Score:4.10|P-value:1.55E-5 ID:SGTC_1947|Compound:st076332|FD-Score:-3.32|P-value:4.21E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.90|P-value:3.80E-5 ID:SGTC_2043|Compound:5101342|FD-Score:-3.18|P-value:6.91E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-4.45|P-value:3.70E-6 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.64|P-value:1.26E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.33|P-value:3.53E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-7.51|P-value:1.82E-14 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.13|P-value:7.37E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.54|P-value:1.63E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.09|P-value:8.52E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.34|P-value:3.97E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.49|P-value:2.26E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.56|P-value:1.84E-6 ID:SGTC_105|Compound:0111-0055|FD-Score:-3.18|P-value:6.90E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.90|P-value:3.80E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:4.84|P-value:4.61E-7 ID:SGTC_709|Compound:3807-1509|FD-Score:7.88|P-value:7.28E-16 ID:SGTC_736|Compound:0958-0254|FD-Score:-3.23|P-value:5.79E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-4.21|P-value:1.12E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:4.22|P-value:9.15E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:4.34|P-value:5.27E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.41|P-value:3.07E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.14|P-value:7.05E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.53|P-value:1.91E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.59|P-value:1.56E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.82|P-value:6.16E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.26|P-value:5.24E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.15|P-value:6.90E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.72|P-value:7.84E-5 ID:SGTC_1387|Compound:3342-1166|FD-Score:-3.40|P-value:3.11E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.24|P-value:5.68E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.86|P-value:4.50E-5 ID:SGTC_1913|Compound:st060213|FD-Score:4.10|P-value:1.55E-5 ID:SGTC_1947|Compound:st076332|FD-Score:-3.32|P-value:4.21E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.90|P-value:3.80E-5 ID:SGTC_2043|Compound:5101342|FD-Score:-3.18|P-value:6.91E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-4.45|P-value:3.70E-6 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.64|P-value:1.26E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.33|P-value:3.53E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-7.51|P-value:1.82E-14 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.13|P-value:7.37E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.54|P-value:1.63E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.09|P-value:8.52E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.34|P-value:3.97E-4

Top fitness defect scores for YDL046W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_709 3807-1509 7.88 243.0 µM 7.28E-16 RSC complex & mRNA processing 1 1.63E-15
2 SGTC_708 3448-5400 4.84 146.0 µM 4.61E-7 21 6.51E-7
3 SGTC_90 0870-0863 4.56 4.6 µM 1.84E-6 32 2.52E-6
4 SGTC_952 1185-0122 4.34 232.0 µM 5.27E-6 14 7.04E-6
5 SGTC_916 4466-0038 4.22 42.0 nM 9.15E-6 cell wall 57 1.21E-5
6 SGTC_1913 st060213 4.10 96.1 µM 1.55E-5 27 2.03E-5
7 SGTC_342 k048-0088 3.90 23.3 µM 3.80E-5 RSC & ERG11 18 4.84E-5
8 SGTC_2001 4023502 3.90 57.7 µM 3.80E-5 mitochondrial processes 19 4.85E-5
9 SGTC_1828 st055811 3.86 49.0 µM 4.50E-5 10 5.72E-5
10 SGTC_1359 1582-0056 3.72 520.0 nM 7.84E-5 25 9.83E-5
11 SGTC_2736 butenafine 3.54 14.7 µM 1.63E-4 41 2.01E-4
12 SGTC_2478 5763493 3.33 32.3 µM 3.53E-4 39 4.27E-4
13 SGTC_1357 1503-1303 3.15 39.5 µM 6.90E-4 77 8.22E-4
14 SGTC_1200 1643-0193 3.14 18.4 µM 7.05E-4 39 8.40E-4
15 SGTC_2531 methyl deoxycholate 3.13 70.1 µM 7.37E-4 60 8.77E-4
16 SGTC_2831 7999036 3.09 19.5 µM 8.52E-4 98 0.00101
17 SGTC_2499 avocadenofuran 3.01 51.5 µM 0.00110 88 0.00129
18 SGTC_943 3253-1379 3.00 105.0 µM 0.00115 65 0.00135
19 SGTC_36 0335-0881 2.98 14.2 µM 0.00123 ubiquinone biosynthesis & proteasome 180 0.00144
20 SGTC_1056 3448-1962 2.94 115.0 µM 0.00142 amide catabolism 62 0.00167

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 9.48E-17 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.122 1.37E-12 YDR053W_d YDR053W_d het Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
0.109 2.25E-10 YDR525W_d API2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.097 1.73E-8 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.091 1.51E-7 YLR336C SGD1 het Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
0.090 1.60E-7 YGR137W_d YGR137W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.088 3.61E-7 YBR085C-A YBR085C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
0.086 6.74E-7 YOL004W SIN3 hom Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
0.085 7.23E-7 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.085 8.87E-7 YBR018C GAL7 hom Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
0.083 1.59E-6 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.082 1.89E-6 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.082 2.03E-6 YDR157W_d YDR157W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 2.17E-6 YDL230W PTP1 hom Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
0.078 5.44E-6 YGR277C CAB4 het Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved