YDL053C / PBP4

Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL053C / PBP4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.91|P-value:6.96E-5 ID:SGTC_169|Compound:k015-0017|FD-Score:6.54|P-value:9.32E-11 ID:SGTC_196|Compound:k015-0023|FD-Score:3.61|P-value:2.26E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:4.20|P-value:2.14E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.50|P-value:3.14E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.47|P-value:6.43E-6 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.32|P-value:6.01E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:4.06|P-value:3.81E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-3.27|P-value:7.13E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.94|P-value:6.34E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.32|P-value:6.02E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:4.25|P-value:1.80E-5 ID:SGTC_784|Compound:4579-0127|FD-Score:-3.91|P-value:6.94E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.56|P-value:2.55E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.28|P-value:6.80E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.39|P-value:9.82E-6 ID:SGTC_1737|Compound:st035557|FD-Score:4.87|P-value:1.08E-6 ID:SGTC_1843|Compound:st055809|FD-Score:3.49|P-value:3.42E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.23|P-value:8.37E-4 ID:SGTC_2031|Compound:5180455|FD-Score:4.14|P-value:2.85E-5 ID:SGTC_2036|Compound:5160441|FD-Score:5.99|P-value:2.77E-9 ID:SGTC_2037|Compound:5169046|FD-Score:-3.41|P-value:4.42E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.86|P-value:8.48E-5 ID:SGTC_2126|Compound:5251174|FD-Score:6.06|P-value:1.86E-9 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.95|P-value:6.02E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.99|P-value:5.25E-5 ID:SGTC_2328|Compound:6201408|FD-Score:4.05|P-value:4.04E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.25|P-value:7.94E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:6.98|P-value:5.31E-12 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:6.55|P-value:8.95E-11 ID:SGTC_2464|Compound:5717415|FD-Score:3.44|P-value:4.14E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.78|P-value:1.67E-6 ID:SGTC_2792|Compound:7535235|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.33|P-value:6.03E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.25|P-value:7.95E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.42|P-value:4.34E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.86|P-value:8.57E-5 ID:SGTC_3106|Compound:9121286|FD-Score:3.25|P-value:7.88E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-5.08|P-value:3.70E-7 ID:SGTC_3119|Compound:9125183|FD-Score:-3.20|P-value:9.03E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.39|P-value:4.67E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.37|P-value:5.07E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.91|P-value:6.96E-5 ID:SGTC_169|Compound:k015-0017|FD-Score:6.54|P-value:9.32E-11 ID:SGTC_196|Compound:k015-0023|FD-Score:3.61|P-value:2.26E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:4.20|P-value:2.14E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.50|P-value:3.14E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.47|P-value:6.43E-6 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.32|P-value:6.01E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:4.06|P-value:3.81E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-3.27|P-value:7.13E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.94|P-value:6.34E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.32|P-value:6.02E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:4.25|P-value:1.80E-5 ID:SGTC_784|Compound:4579-0127|FD-Score:-3.91|P-value:6.94E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.56|P-value:2.55E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.28|P-value:6.80E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.39|P-value:9.82E-6 ID:SGTC_1737|Compound:st035557|FD-Score:4.87|P-value:1.08E-6 ID:SGTC_1843|Compound:st055809|FD-Score:3.49|P-value:3.42E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.23|P-value:8.37E-4 ID:SGTC_2031|Compound:5180455|FD-Score:4.14|P-value:2.85E-5 ID:SGTC_2036|Compound:5160441|FD-Score:5.99|P-value:2.77E-9 ID:SGTC_2037|Compound:5169046|FD-Score:-3.41|P-value:4.42E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.86|P-value:8.48E-5 ID:SGTC_2126|Compound:5251174|FD-Score:6.06|P-value:1.86E-9 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.95|P-value:6.02E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.99|P-value:5.25E-5 ID:SGTC_2328|Compound:6201408|FD-Score:4.05|P-value:4.04E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.25|P-value:7.94E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:6.98|P-value:5.31E-12 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:6.55|P-value:8.95E-11 ID:SGTC_2464|Compound:5717415|FD-Score:3.44|P-value:4.14E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.78|P-value:1.67E-6 ID:SGTC_2792|Compound:7535235|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.33|P-value:6.03E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.25|P-value:7.95E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.42|P-value:4.34E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.86|P-value:8.57E-5 ID:SGTC_3106|Compound:9121286|FD-Score:3.25|P-value:7.88E-4 ID:SGTC_3114|Compound:9122766|FD-Score:-5.08|P-value:3.70E-7 ID:SGTC_3119|Compound:9125183|FD-Score:-3.20|P-value:9.03E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.39|P-value:4.67E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.37|P-value:5.07E-4

Top fitness defect scores for YDL053C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2407 paclitaxel 6.98 400.0 µM 5.31E-12 6 1.46E-12
2 SGTC_2410 aloe-emodin-8-o-glycoside 6.55 46.3 µM 8.95E-11 mitochondrial processes 4 2.86E-11
3 SGTC_169 k015-0017 6.54 16.0 µM 9.32E-11 1 2.99E-11
4 SGTC_2126 5251174 6.06 33.9 µM 1.86E-9 2 6.97E-10
5 SGTC_2036 5160441 5.99 105.0 µM 2.77E-9 NEO1 43 1.06E-9
6 SGTC_1737 st035557 4.87 69.7 µM 1.08E-6 9 5.68E-7
7 SGTC_2541 muurolladie-3-one 4.78 83.1 µM 1.67E-6 excess fatty acid 12 8.98E-7
8 SGTC_1727 st034309 4.39 9.9 µM 9.82E-6 24 5.78E-6
9 SGTC_775 4577-0580 4.25 57.3 µM 1.80E-5 5 1.09E-5
10 SGTC_198 3454-2873 4.20 16.2 µM 2.14E-5 60S ribosome export 11 1.31E-5
11 SGTC_2031 5180455 4.14 200.0 µM 2.85E-5 10 1.77E-5
12 SGTC_425 3825-7017 4.06 13.1 µM 3.81E-5 16 2.40E-5
13 SGTC_2328 6201408 4.05 200.0 µM 4.04E-5 18 2.56E-5
14 SGTC_2326 6164455 3.99 177.6 µM 5.25E-5 mitochondrial processes 20 3.37E-5
15 SGTC_2249 haloperidol 3.95 116.4 µM 6.02E-5 ergosterol depletion effects on membrane 113 3.89E-5
16 SGTC_439 amiodarone 3.94 34.4 µM 6.34E-5 NEO1-PIK1 99 4.11E-5
17 SGTC_2095 5432005 3.86 200.0 µM 8.48E-5 10 5.58E-5
18 SGTC_3104 9120930 3.86 49.5 µM 8.57E-5 14 5.64E-5
19 SGTC_196 k015-0023 3.61 18.1 µM 2.26E-4 PDR1 35 1.56E-4
20 SGTC_1843 st055809 3.49 45.5 µM 3.42E-4 28 2.42E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 3.01E-14 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.126 2.35E-13 YGR178C PBP1 hom Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.113 5.14E-11 YGL161C YIP5 hom Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
0.111 1.29E-10 YBR222C PCS60 hom Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
0.110 1.46E-10 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.108 3.24E-10 YER157W COG3 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.099 1.01E-8 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.098 1.11E-8 YGL253W HXK2 hom Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication
0.096 2.19E-8 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.095 3.03E-8 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.095 3.56E-8 YOR070C GYP1 hom Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.092 8.43E-8 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.091 1.28E-7 YBR249C ARO4 hom 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
0.091 1.39E-7 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.089 2.40E-7 YEL008W_d YEL008W_d hom Hypothetical protein predicted to be involved in metabolism