YDL058W / USO1

Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL058W / USO1

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:4.37|P-value:1.65E-5 ID:SGTC_17|Compound:0960-0276|FD-Score:3.51|P-value:4.46E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:4.22|P-value:3.01E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:3.54|P-value:3.95E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.28|P-value:9.36E-4 ID:SGTC_393|Compound:acivicin|FD-Score:4.03|P-value:6.42E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:4.22|P-value:3.03E-5 ID:SGTC_470|Compound:aa-861|FD-Score:4.15|P-value:3.94E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.60|P-value:4.72E-8 ID:SGTC_569|Compound:4130-2812|FD-Score:3.54|P-value:3.99E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:8.66|P-value:5.55E-17 ID:SGTC_681|Compound:1642-0006|FD-Score:3.31|P-value:8.62E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:-3.29|P-value:6.24E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.76|P-value:1.80E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.31|P-value:8.66E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:4.03|P-value:6.43E-5 ID:SGTC_721|Compound:1187-1332|FD-Score:3.28|P-value:9.39E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.58|P-value:1.59E-10 ID:SGTC_901|Compound:1112-0062|FD-Score:4.43|P-value:1.23E-5 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.21|P-value:1.55E-9 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.40|P-value:6.40E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:5.18|P-value:3.91E-7 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.49|P-value:4.69E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.28|P-value:9.48E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.40|P-value:6.28E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.47|P-value:1.07E-5 ID:SGTC_1617|Compound:st002906|FD-Score:-3.31|P-value:5.83E-4 ID:SGTC_1732|Compound:st034308|FD-Score:4.85|P-value:1.92E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.45|P-value:5.36E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.46|P-value:5.29E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.43|P-value:5.71E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.61|P-value:3.12E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.62|P-value:2.98E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.42|P-value:5.88E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.30|P-value:8.82E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.70|P-value:2.26E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.89|P-value:1.12E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.82|P-value:2.18E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.58|P-value:3.94E-6 ID:SGTC_3040|Compound:9091894|FD-Score:5.60|P-value:4.51E-8 ID:SGTC_3138|Compound:9097216|FD-Score:3.82|P-value:1.43E-4 ID:SGTC_3155|Compound:9099265|FD-Score:3.38|P-value:6.92E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:4.37|P-value:1.65E-5 ID:SGTC_17|Compound:0960-0276|FD-Score:3.51|P-value:4.46E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:4.22|P-value:3.01E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:3.54|P-value:3.95E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.28|P-value:9.36E-4 ID:SGTC_393|Compound:acivicin|FD-Score:4.03|P-value:6.42E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:4.22|P-value:3.03E-5 ID:SGTC_470|Compound:aa-861|FD-Score:4.15|P-value:3.94E-5 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.60|P-value:4.72E-8 ID:SGTC_569|Compound:4130-2812|FD-Score:3.54|P-value:3.99E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:8.66|P-value:5.55E-17 ID:SGTC_681|Compound:1642-0006|FD-Score:3.31|P-value:8.62E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:-3.29|P-value:6.24E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.76|P-value:1.80E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.31|P-value:8.66E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:4.03|P-value:6.43E-5 ID:SGTC_721|Compound:1187-1332|FD-Score:3.28|P-value:9.39E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.58|P-value:1.59E-10 ID:SGTC_901|Compound:1112-0062|FD-Score:4.43|P-value:1.23E-5 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.21|P-value:1.55E-9 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.40|P-value:6.40E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:5.18|P-value:3.91E-7 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.49|P-value:4.69E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.28|P-value:9.48E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.40|P-value:6.28E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.47|P-value:1.07E-5 ID:SGTC_1617|Compound:st002906|FD-Score:-3.31|P-value:5.83E-4 ID:SGTC_1732|Compound:st034308|FD-Score:4.85|P-value:1.92E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.45|P-value:5.36E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.46|P-value:5.29E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.43|P-value:5.71E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.61|P-value:3.12E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.62|P-value:2.98E-4 ID:SGTC_2120|Compound:5538279|FD-Score:3.42|P-value:5.88E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.30|P-value:8.82E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.70|P-value:2.26E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.89|P-value:1.12E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.82|P-value:2.18E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.58|P-value:3.94E-6 ID:SGTC_3040|Compound:9091894|FD-Score:5.60|P-value:4.51E-8 ID:SGTC_3138|Compound:9097216|FD-Score:3.82|P-value:1.43E-4 ID:SGTC_3155|Compound:9099265|FD-Score:3.38|P-value:6.92E-4

Top fitness defect scores for YDL058W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_633 0437-0093 8.66 97.7 µM 5.55E-17 DNA intercalators 1 2.33E-18 1.57
2 SGTC_867 0518-0118 6.58 155.0 µM 1.59E-10 DNA intercalators 3 2.29E-11 0.26
3 SGTC_1060 3448-7350 6.21 82.0 µM 1.55E-9 Golgi 7 2.71E-10 0.10
4 SGTC_3040 9091894 5.60 49.5 µM 4.51E-8 DNA intercalators 4 1.05E-8 0.45
5 SGTC_471 1-acyl-paf 5.60 4.0 µM 4.72E-8 DNA intercalators 9 1.10E-8 0.03
6 SGTC_1290 0986-0249 5.18 10.2 µM 3.91E-7 DNA intercalators 3 1.09E-7 0.18
7 SGTC_1732 st034308 4.85 51.8 µM 1.92E-6 3 6.13E-7 0.16
8 SGTC_2536 dehydroabietamide 4.82 32.0 µM 2.18E-6 4 7.04E-7 0.20
9 SGTC_1459 k035-0031 4.47 35.7 µM 1.07E-5 DNA intercalators 8 3.98E-6 0.03
10 SGTC_901 1112-0062 4.43 60.1 µM 1.23E-5 4 4.63E-6 0.58
11 SGTC_9 0092-0002 4.37 10.4 µM 1.65E-5 10 6.35E-6 0.03
12 SGTC_96 0929-0093 4.22 37.3 µM 3.01E-5 calcium & mitochondrial duress 3 1.22E-5 0.28
13 SGTC_412 gemfibrozil 4.22 100.0 µM 3.03E-5 DNA intercalators 5 1.23E-5 0.23
14 SGTC_470 aa-861 4.15 153.0 µM 3.94E-5 sphingolipid biosynthesis & PDR1 13 1.63E-5 0.36
15 SGTC_393 acivicin 4.03 6.2 µM 6.42E-5 10 2.78E-5 0.05
16 SGTC_717 1532-0246 4.03 73.2 µM 6.43E-5 Golgi 7 2.78E-5 0.20
17 SGTC_2489 cantharidin 3.89 73.4 µM 1.12E-4 cell wall signaling 7 5.06E-5 0.12
18 SGTC_3138 9097216 3.82 49.5 µM 1.43E-4 7 6.64E-5 0.10
19 SGTC_700 3453-2283 3.76 142.0 µM 1.80E-4 amide catabolism 9 8.52E-5 0.26
20 SGTC_2473 5854593 3.70 8.9 µM 2.26E-4 4 1.09E-4 0.34

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.386 1.23E-119 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.365 1.72E-105 YMR119W ASI1 hom Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
0.355 2.74E-100 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.324 1.09E-82 YOR278W HEM4 het Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
0.321 3.49E-81 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.321 3.96E-81 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.315 6.70E-78 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.309 6.48E-75 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.308 1.01E-74 YDR362C TFC6 het One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
0.290 4.78E-66 YDR458C HEH2 hom Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
0.282 1.72E-62 YNL317W PFS2 het Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
0.228 1.05E-40 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.208 4.42E-34 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.187 1.02E-27 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.185 3.28E-27 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p