YDL059C / RAD59

Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL059C / RAD59

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:-3.42|P-value:6.94E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.85|P-value:1.72E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.49|P-value:5.88E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.79|P-value:2.07E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.86|P-value:1.62E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.86|P-value:1.64E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:6.51|P-value:6.66E-10 ID:SGTC_248|Compound:streptozotocin|FD-Score:7.06|P-value:2.43E-11 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.14|P-value:5.20E-9 ID:SGTC_268|Compound:camptothecin|FD-Score:10.20|P-value:1.42E-21 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.84|P-value:1.64E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.66|P-value:7.17E-6 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.52|P-value:5.32E-4 ID:SGTC_459|Compound:nigericin|FD-Score:-4.31|P-value:2.83E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.45|P-value:6.14E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.67|P-value:3.21E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:7.22|P-value:8.50E-12 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.68|P-value:2.87E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:6.42|P-value:1.14E-9 ID:SGTC_827|Compound:3253-0474|FD-Score:-4.49|P-value:1.37E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:12.40|P-value:2.12E-31 ID:SGTC_858|Compound:0438-0351|FD-Score:3.46|P-value:6.50E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:12.30|P-value:6.23E-31 ID:SGTC_1082|Compound:camptothecin|FD-Score:21.90|P-value:5.03E-93 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.05|P-value:7.60E-5 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.54|P-value:1.16E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.93|P-value:1.26E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.33|P-value:9.88E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.23|P-value:4.10E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.34|P-value:2.72E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:4.19|P-value:4.75E-5 ID:SGTC_1707|Compound:st032323|FD-Score:28.20|P-value:6.20E-153 ID:SGTC_1718|Compound:roemerine|FD-Score:5.18|P-value:7.10E-7 ID:SGTC_1763|Compound:st044482|FD-Score:3.47|P-value:6.30E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.42|P-value:1.95E-5 ID:SGTC_1913|Compound:st060213|FD-Score:5.33|P-value:3.42E-7 ID:SGTC_1915|Compound:st067105|FD-Score:5.31|P-value:3.81E-7 ID:SGTC_1930|Compound:st074634|FD-Score:3.44|P-value:6.90E-4 ID:SGTC_2087|Compound:5276107|FD-Score:3.57|P-value:4.55E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.73|P-value:2.64E-4 ID:SGTC_2100|Compound:5377183|FD-Score:-3.33|P-value:9.20E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_2190|Compound:6049624|FD-Score:-4.25|P-value:3.51E-5 ID:SGTC_2239|Compound:6685329|FD-Score:3.41|P-value:7.57E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.85|P-value:1.59E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.87|P-value:1.58E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:14.00|P-value:2.63E-39 ID:SGTC_2671|Compound:danthron|FD-Score:5.47|P-value:1.76E-7 ID:SGTC_2834|Compound:9001755|FD-Score:5.49|P-value:1.57E-7 ID:SGTC_2839|Compound:9003951|FD-Score:4.07|P-value:7.53E-5 ID:SGTC_2913|Compound:7966556|FD-Score:-3.35|P-value:8.49E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.43|P-value:6.70E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.78|P-value:2.19E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.36|P-value:8.74E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.61|P-value:3.85E-4 ID:SGTC_3116|Compound:9124943|FD-Score:5.03|P-value:1.42E-6 ID:SGTC_3119|Compound:9125183|FD-Score:3.70|P-value:2.91E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.03|P-value:2.93E-11 ID:SGTC_3254|Compound:9136651|FD-Score:5.88|P-value:2.21E-8 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.42|P-value:6.94E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:3.85|P-value:1.72E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.49|P-value:5.88E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.79|P-value:2.07E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.86|P-value:1.62E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.86|P-value:1.64E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:6.51|P-value:6.66E-10 ID:SGTC_248|Compound:streptozotocin|FD-Score:7.06|P-value:2.43E-11 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.14|P-value:5.20E-9 ID:SGTC_268|Compound:camptothecin|FD-Score:10.20|P-value:1.42E-21 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.84|P-value:1.64E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.66|P-value:7.17E-6 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.52|P-value:5.32E-4 ID:SGTC_459|Compound:nigericin|FD-Score:-4.31|P-value:2.83E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.45|P-value:6.14E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.67|P-value:3.21E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:7.22|P-value:8.50E-12 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.68|P-value:2.87E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:6.42|P-value:1.14E-9 ID:SGTC_827|Compound:3253-0474|FD-Score:-4.49|P-value:1.37E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:12.40|P-value:2.12E-31 ID:SGTC_858|Compound:0438-0351|FD-Score:3.46|P-value:6.50E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:12.30|P-value:6.23E-31 ID:SGTC_1082|Compound:camptothecin|FD-Score:21.90|P-value:5.03E-93 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.05|P-value:7.60E-5 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.54|P-value:1.16E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.93|P-value:1.26E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.33|P-value:9.88E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.23|P-value:4.10E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.34|P-value:2.72E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:4.19|P-value:4.75E-5 ID:SGTC_1707|Compound:st032323|FD-Score:28.20|P-value:6.20E-153 ID:SGTC_1718|Compound:roemerine|FD-Score:5.18|P-value:7.10E-7 ID:SGTC_1763|Compound:st044482|FD-Score:3.47|P-value:6.30E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.42|P-value:1.95E-5 ID:SGTC_1913|Compound:st060213|FD-Score:5.33|P-value:3.42E-7 ID:SGTC_1915|Compound:st067105|FD-Score:5.31|P-value:3.81E-7 ID:SGTC_1930|Compound:st074634|FD-Score:3.44|P-value:6.90E-4 ID:SGTC_2087|Compound:5276107|FD-Score:3.57|P-value:4.55E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.73|P-value:2.64E-4 ID:SGTC_2100|Compound:5377183|FD-Score:-3.33|P-value:9.20E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_2190|Compound:6049624|FD-Score:-4.25|P-value:3.51E-5 ID:SGTC_2239|Compound:6685329|FD-Score:3.41|P-value:7.57E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.85|P-value:1.59E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.87|P-value:1.58E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:14.00|P-value:2.63E-39 ID:SGTC_2671|Compound:danthron|FD-Score:5.47|P-value:1.76E-7 ID:SGTC_2834|Compound:9001755|FD-Score:5.49|P-value:1.57E-7 ID:SGTC_2839|Compound:9003951|FD-Score:4.07|P-value:7.53E-5 ID:SGTC_2913|Compound:7966556|FD-Score:-3.35|P-value:8.49E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.43|P-value:6.70E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.78|P-value:2.19E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.36|P-value:8.74E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.61|P-value:3.85E-4 ID:SGTC_3116|Compound:9124943|FD-Score:5.03|P-value:1.42E-6 ID:SGTC_3119|Compound:9125183|FD-Score:3.70|P-value:2.91E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.03|P-value:2.93E-11 ID:SGTC_3254|Compound:9136651|FD-Score:5.88|P-value:2.21E-8

Top fitness defect scores for YDL059C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1707 st032323 28.20 6.3 µM 6.20E-153 DNA damage response 1 1.86E-175
2 SGTC_1082 camptothecin 21.90 424.9 nM 5.03E-93 DNA damage response 1 1.26E-106
3 SGTC_2612 dictamnine 14.00 15.5 µM 2.63E-39 RNA pol III & RNase P/MRP 1 6.56E-45
4 SGTC_829 1635-0120 12.40 117.0 µM 2.12E-31 DNA damage response 4 7.90E-36
5 SGTC_1079 idarubicin 12.30 2.4 µM 6.23E-31 DNA damage response 1 2.73E-35
6 SGTC_268 camptothecin 10.20 39.6 µM 1.42E-21 DNA damage response 9 1.53E-24
7 SGTC_558 k064-0027 7.22 21.3 µM 8.50E-12 DNA damage response 3 2.55E-13
8 SGTC_248 streptozotocin 7.06 5.2 mM 2.43E-11 DNA damage response 7 8.48E-13
9 SGTC_3247 9135149 7.03 49.5 µM 2.93E-11 DNA damage response 9 1.05E-12
10 SGTC_244 nsc-207895 6.51 1.3 µM 6.66E-10 DNA damage response 8 3.79E-11
11 SGTC_824 1304-0008 6.42 100.0 µM 1.14E-9 DNA damage response 8 7.01E-11
12 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 6.14 44.4 nM 5.20E-9 DNA damage response 19 4.00E-10
13 SGTC_3254 9136651 5.88 49.5 µM 2.21E-8 DNA damage response 4 2.10E-9
14 SGTC_2834 9001755 5.49 58.4 µM 1.57E-7 10 1.99E-8
15 SGTC_2671 danthron 5.47 100.0 µM 1.76E-7 DNA damage response 17 2.27E-8
16 SGTC_1913 st060213 5.33 96.1 µM 3.42E-7 9 4.85E-8
17 SGTC_1915 st067105 5.31 30.6 µM 3.81E-7 RNA pol III & RNase P/MRP 12 5.49E-8
18 SGTC_1718 roemerine 5.18 11.9 µM 7.10E-7 redox potentiating 20 1.12E-7
19 SGTC_3116 9124943 5.03 49.5 µM 1.42E-6 6 2.48E-7
20 SGTC_411 enrofloxacin 4.66 100.0 µM 7.17E-6 DNA damage response 3 1.58E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.608 0 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.604 0 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.551 8.52E-266 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.549 2.21E-263 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.536 8.01E-249 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.527 1.56E-239 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.522 2.34E-234 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.455 5.32E-171 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.360 2.32E-103 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.356 1.35E-100 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.316 1.40E-78 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.308 1.92E-74 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.294 5.67E-68 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.289 2.12E-65 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.242 4.32E-46 YDR369C XRS2 hom Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling