YDL074C / BRE1

E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL074C / BRE1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:6.53|P-value:1.72E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:6.69|P-value:8.99E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:4.79|P-value:9.70E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:7.75|P-value:6.98E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.98|P-value:3.72E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:4.01|P-value:9.27E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:7.33|P-value:5.17E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:8.42|P-value:2.25E-11 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.43|P-value:2.09E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:6.63|P-value:1.16E-7 ID:SGTC_200|Compound:k038-0079|FD-Score:5.74|P-value:3.80E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.28|P-value:4.40E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.46|P-value:2.62E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.60|P-value:4.36E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:8.84|P-value:2.32E-12 ID:SGTC_851|Compound:0394-0702|FD-Score:-5.29|P-value:1.31E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:4.07|P-value:7.90E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-5.74|P-value:2.56E-6 ID:SGTC_923|Compound:1900-2434|FD-Score:8.79|P-value:3.11E-12 ID:SGTC_930|Compound:2810-3379|FD-Score:4.82|P-value:8.81E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-4.48|P-value:1.78E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.88|P-value:7.20E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:-4.65|P-value:1.07E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.70|P-value:1.28E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:4.06|P-value:8.14E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.01|P-value:9.32E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.06|P-value:8.13E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:5.16|P-value:2.95E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.66|P-value:1.42E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.26|P-value:3.44E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-4.28|P-value:3.22E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-6.45|P-value:1.54E-7 ID:SGTC_2090|Compound:5301565|FD-Score:5.46|P-value:1.03E-5 ID:SGTC_2111|Compound:5486399|FD-Score:4.56|P-value:1.97E-4 ID:SGTC_2297|Compound:7971671|FD-Score:4.58|P-value:1.81E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:6.78|P-value:6.07E-8 ID:SGTC_2408|Compound:sulbentine|FD-Score:8.37|P-value:3.00E-11 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.05|P-value:8.28E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:-4.48|P-value:1.82E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.54|P-value:2.04E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.31|P-value:4.04E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.33|P-value:3.79E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.02|P-value:8.88E-4 ID:SGTC_3140|Compound:9094243|FD-Score:5.98|P-value:1.56E-6 ID:SGTC_3143|Compound:9086658|FD-Score:5.37|P-value:1.43E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.90|P-value:9.25E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-4.19|P-value:4.22E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.77|P-value:1.04E-4 ID:SGTC_3355|Compound:9151058|FD-Score:4.94|P-value:6.06E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:6.53|P-value:1.72E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:6.69|P-value:8.99E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:4.79|P-value:9.70E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:7.75|P-value:6.98E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.98|P-value:3.72E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:4.01|P-value:9.27E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:7.33|P-value:5.17E-9 ID:SGTC_158|Compound:k038-0087|FD-Score:8.42|P-value:2.25E-11 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.43|P-value:2.09E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:6.63|P-value:1.16E-7 ID:SGTC_200|Compound:k038-0079|FD-Score:5.74|P-value:3.80E-6 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.28|P-value:4.40E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.46|P-value:2.62E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.60|P-value:4.36E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:8.84|P-value:2.32E-12 ID:SGTC_851|Compound:0394-0702|FD-Score:-5.29|P-value:1.31E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:4.07|P-value:7.90E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-5.74|P-value:2.56E-6 ID:SGTC_923|Compound:1900-2434|FD-Score:8.79|P-value:3.11E-12 ID:SGTC_930|Compound:2810-3379|FD-Score:4.82|P-value:8.81E-5 ID:SGTC_933|Compound:2914-0560|FD-Score:-4.48|P-value:1.78E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.88|P-value:7.20E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:-4.65|P-value:1.07E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.70|P-value:1.28E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:4.06|P-value:8.14E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.01|P-value:9.32E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.06|P-value:8.13E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:5.16|P-value:2.95E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.66|P-value:1.42E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.26|P-value:3.44E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-4.28|P-value:3.22E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-6.45|P-value:1.54E-7 ID:SGTC_2090|Compound:5301565|FD-Score:5.46|P-value:1.03E-5 ID:SGTC_2111|Compound:5486399|FD-Score:4.56|P-value:1.97E-4 ID:SGTC_2297|Compound:7971671|FD-Score:4.58|P-value:1.81E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:6.78|P-value:6.07E-8 ID:SGTC_2408|Compound:sulbentine|FD-Score:8.37|P-value:3.00E-11 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.05|P-value:8.28E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:-4.48|P-value:1.82E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.54|P-value:2.04E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.31|P-value:4.04E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.33|P-value:3.79E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.02|P-value:8.88E-4 ID:SGTC_3140|Compound:9094243|FD-Score:5.98|P-value:1.56E-6 ID:SGTC_3143|Compound:9086658|FD-Score:5.37|P-value:1.43E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.90|P-value:9.25E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-4.19|P-value:4.22E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.77|P-value:1.04E-4 ID:SGTC_3355|Compound:9151058|FD-Score:4.94|P-value:6.06E-5

Top fitness defect scores for YDL074C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_439 amiodarone 8.84 34.4 µM 2.32E-12 NEO1-PIK1 18 4.72E-19
2 SGTC_923 1900-2434 8.79 73.3 µM 3.11E-12 RNA processing & uracil transport 3 7.58E-19
3 SGTC_158 k038-0087 8.42 26.4 µM 2.25E-11 ubiquinone biosynthesis & proteasome 46 1.84E-17
4 SGTC_2408 sulbentine 8.37 18.4 µM 3.00E-11 11 2.92E-17
5 SGTC_86 0417-1630 7.75 471.9 µM 6.98E-10 ubiquinone biosynthesis & proteasome 61 4.65E-15
6 SGTC_142 0335-0849 7.33 62.7 µM 5.17E-9 ubiquinone biosynthesis & proteasome 47 1.17E-13
7 SGTC_2405 ticlatone 6.78 3.5 µM 6.07E-8 21 6.13E-12
8 SGTC_58 0957-0393 6.69 9.0 µM 8.99E-8 cell wall 175 1.15E-11
9 SGTC_165 k038-0023 6.63 64.4 µM 1.16E-7 ubiquinone biosynthesis & proteasome 16 1.73E-11
10 SGTC_36 0335-0881 6.53 14.2 µM 1.72E-7 ubiquinone biosynthesis & proteasome 52 3.26E-11
11 SGTC_3140 9094243 5.98 49.5 µM 1.56E-6 5 1.13E-9
12 SGTC_200 k038-0079 5.74 37.5 µM 3.80E-6 ubiquinone biosynthesis & proteasome 29 4.70E-9
13 SGTC_2090 5301565 5.46 104.5 µM 1.03E-5 TSC3-RPN4 22 2.32E-8
14 SGTC_3143 9086658 5.37 49.5 µM 1.43E-5 13 3.93E-8
15 SGTC_1537 2',6'-dihydroxyflavanone 5.16 78.0 µM 2.95E-5 2 1.26E-7
16 SGTC_3355 9151058 4.94 44.0 µM 6.06E-5 RNA processing & uracil transport 2 3.96E-7
17 SGTC_1002 4049-0206 4.88 172.0 µM 7.20E-5 12 5.22E-7
18 SGTC_930 2810-3379 4.82 265.0 µM 8.81E-5 4 7.19E-7
19 SGTC_64 000l-0018 4.79 272.1 µM 9.70E-5 ubiquinone biosynthesis & proteasome 114 8.39E-7
20 SGTC_3321 9137793 4.77 26.8 µM 1.04E-4 cell wall 49 9.31E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.295 2.14E-68 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.291 1.58E-66 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.288 4.77E-65 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.283 1.05E-62 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.280 1.39E-61 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.280 1.44E-61 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.277 4.60E-60 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.275 2.58E-59 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.267 1.36E-55 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.259 2.06E-52 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.252 7.09E-50 YPR065W ROX1 hom Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.245 5.81E-47 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.240 4.64E-45 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.238 2.81E-44 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.238 2.89E-44 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron