YDL077C / VAM6

Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL077C / VAM6

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:4.90|P-value:4.41E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:5.65|P-value:3.12E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:4.26|P-value:3.36E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:9.09|P-value:1.64E-13 ID:SGTC_123|Compound:0083-0120|FD-Score:6.86|P-value:1.96E-8 ID:SGTC_139|Compound:0099-0334|FD-Score:8.98|P-value:3.10E-13 ID:SGTC_149|Compound:3970-0822|FD-Score:6.57|P-value:7.32E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.75|P-value:3.24E-8 ID:SGTC_232|Compound:clozapine|FD-Score:5.98|P-value:8.52E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.73|P-value:2.31E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.85|P-value:9.34E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:4.08|P-value:5.59E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.93|P-value:8.55E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.64|P-value:2.67E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:-5.52|P-value:4.26E-6 ID:SGTC_332|Compound:4112-0506|FD-Score:5.15|P-value:1.92E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.68|P-value:9.09E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:10.90|P-value:8.55E-19 ID:SGTC_352|Compound:k048-0007|FD-Score:5.41|P-value:7.55E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.58|P-value:6.78E-8 ID:SGTC_435|Compound:c-PAF|FD-Score:-6.61|P-value:5.03E-8 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.81|P-value:1.67E-6 ID:SGTC_459|Compound:nigericin|FD-Score:8.05|P-value:5.74E-11 ID:SGTC_481|Compound:bay k-8644|FD-Score:4.29|P-value:3.01E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:-4.14|P-value:4.16E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.88|P-value:1.82E-8 ID:SGTC_554|Compound:4483-2211|FD-Score:4.31|P-value:2.86E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:7.34|P-value:2.05E-9 ID:SGTC_687|Compound:k029-0044|FD-Score:5.17|P-value:1.79E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.15|P-value:4.55E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.66|P-value:9.87E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:4.50|P-value:1.62E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:16.60|P-value:8.20E-41 ID:SGTC_848|Compound:0368-0089|FD-Score:4.00|P-value:6.90E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:4.84|P-value:5.36E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.71|P-value:8.27E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.79|P-value:6.34E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:3.94|P-value:8.31E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.29|P-value:2.64E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.08|P-value:5.65E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.14|P-value:4.45E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.26|P-value:3.32E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.57|P-value:1.29E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-6.23|P-value:2.58E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:-5.59|P-value:3.25E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.09|P-value:2.36E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.59|P-value:3.79E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:4.81|P-value:6.01E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:5.03|P-value:2.87E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:11.70|P-value:3.05E-21 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.95|P-value:8.07E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.93|P-value:8.52E-4 ID:SGTC_1658|Compound:st012944|FD-Score:8.82|P-value:7.94E-13 ID:SGTC_1660|Compound:st012960|FD-Score:5.43|P-value:6.94E-6 ID:SGTC_1687|Compound:st020778|FD-Score:13.20|P-value:1.48E-26 ID:SGTC_1750|Compound:st081859|FD-Score:4.81|P-value:6.03E-5 ID:SGTC_1786|Compound:berberine|FD-Score:-4.34|P-value:2.29E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:5.43|P-value:7.10E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.06|P-value:2.61E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.70|P-value:4.72E-18 ID:SGTC_1873|Compound:rapamycin|FD-Score:12.60|P-value:3.41E-24 ID:SGTC_1877|Compound:wortmannin|FD-Score:9.15|P-value:1.13E-13 ID:SGTC_1887|Compound:st057648|FD-Score:-3.88|P-value:8.66E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-5.05|P-value:2.32E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.90|P-value:9.26E-4 ID:SGTC_2029|Compound:5175081|FD-Score:4.44|P-value:1.92E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.57|P-value:1.30E-4 ID:SGTC_2077|Compound:5212015|FD-Score:9.55|P-value:9.80E-15 ID:SGTC_2090|Compound:5301565|FD-Score:4.48|P-value:1.72E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.95|P-value:7.96E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.51|P-value:1.56E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.13|P-value:4.86E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.60|P-value:1.18E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.81|P-value:5.18E-5 ID:SGTC_2275|Compound:7947463|FD-Score:7.35|P-value:1.99E-9 ID:SGTC_2291|Compound:7917025|FD-Score:3.89|P-value:9.35E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.34|P-value:2.62E-4 ID:SGTC_2322|Compound:7413319|FD-Score:13.40|P-value:2.00E-27 ID:SGTC_2389|Compound:9091542|FD-Score:9.50|P-value:1.30E-14 ID:SGTC_2459|Compound:5811157|FD-Score:4.39|P-value:2.23E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.33|P-value:2.70E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.07|P-value:5.79E-4 ID:SGTC_2481|Compound:5478948|FD-Score:6.24|P-value:3.00E-7 ID:SGTC_2482|Compound:5981709|FD-Score:4.63|P-value:1.08E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:19.10|P-value:4.31E-53 ID:SGTC_2526|Compound:patulin|FD-Score:4.92|P-value:4.22E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:3.90|P-value:9.26E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:7.47|P-value:1.11E-9 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.01|P-value:6.70E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:5.60|P-value:3.65E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.80|P-value:3.21E-18 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.19|P-value:4.10E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:9.13|P-value:1.27E-13 ID:SGTC_2743|Compound:auranofin|FD-Score:7.29|P-value:2.58E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.75|P-value:7.30E-5 ID:SGTC_2927|Compound:9011802|FD-Score:4.32|P-value:2.75E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.80|P-value:2.62E-8 ID:SGTC_3131|Compound:9127911|FD-Score:-4.72|P-value:7.04E-5 ID:SGTC_3133|Compound:9127736|FD-Score:-4.32|P-value:2.45E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.16|P-value:4.41E-4 ID:SGTC_3324|Compound:9138759|FD-Score:5.87|P-value:1.33E-6 ID:SGTC_3330|Compound:9141424|FD-Score:3.88|P-value:9.70E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:4.90|P-value:4.41E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:5.65|P-value:3.12E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:4.26|P-value:3.36E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:9.09|P-value:1.64E-13 ID:SGTC_123|Compound:0083-0120|FD-Score:6.86|P-value:1.96E-8 ID:SGTC_139|Compound:0099-0334|FD-Score:8.98|P-value:3.10E-13 ID:SGTC_149|Compound:3970-0822|FD-Score:6.57|P-value:7.32E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.75|P-value:3.24E-8 ID:SGTC_232|Compound:clozapine|FD-Score:5.98|P-value:8.52E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.73|P-value:2.31E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.85|P-value:9.34E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:4.08|P-value:5.59E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.93|P-value:8.55E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.64|P-value:2.67E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:-5.52|P-value:4.26E-6 ID:SGTC_332|Compound:4112-0506|FD-Score:5.15|P-value:1.92E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.68|P-value:9.09E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:10.90|P-value:8.55E-19 ID:SGTC_352|Compound:k048-0007|FD-Score:5.41|P-value:7.55E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.58|P-value:6.78E-8 ID:SGTC_435|Compound:c-PAF|FD-Score:-6.61|P-value:5.03E-8 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.81|P-value:1.67E-6 ID:SGTC_459|Compound:nigericin|FD-Score:8.05|P-value:5.74E-11 ID:SGTC_481|Compound:bay k-8644|FD-Score:4.29|P-value:3.01E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:-4.14|P-value:4.16E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.88|P-value:1.82E-8 ID:SGTC_554|Compound:4483-2211|FD-Score:4.31|P-value:2.86E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:7.34|P-value:2.05E-9 ID:SGTC_687|Compound:k029-0044|FD-Score:5.17|P-value:1.79E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.15|P-value:4.55E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.66|P-value:9.87E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:4.50|P-value:1.62E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:16.60|P-value:8.20E-41 ID:SGTC_848|Compound:0368-0089|FD-Score:4.00|P-value:6.90E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:4.84|P-value:5.36E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.71|P-value:8.27E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.79|P-value:6.34E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:3.94|P-value:8.31E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.29|P-value:2.64E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.08|P-value:5.65E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.14|P-value:4.45E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.26|P-value:3.32E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.57|P-value:1.29E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-6.23|P-value:2.58E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:-5.59|P-value:3.25E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.09|P-value:2.36E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.59|P-value:3.79E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:4.81|P-value:6.01E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:5.03|P-value:2.87E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:11.70|P-value:3.05E-21 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.95|P-value:8.07E-4 ID:SGTC_1622|Compound:st003707|FD-Score:3.93|P-value:8.52E-4 ID:SGTC_1658|Compound:st012944|FD-Score:8.82|P-value:7.94E-13 ID:SGTC_1660|Compound:st012960|FD-Score:5.43|P-value:6.94E-6 ID:SGTC_1687|Compound:st020778|FD-Score:13.20|P-value:1.48E-26 ID:SGTC_1750|Compound:st081859|FD-Score:4.81|P-value:6.03E-5 ID:SGTC_1786|Compound:berberine|FD-Score:-4.34|P-value:2.29E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:5.43|P-value:7.10E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.06|P-value:2.61E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.70|P-value:4.72E-18 ID:SGTC_1873|Compound:rapamycin|FD-Score:12.60|P-value:3.41E-24 ID:SGTC_1877|Compound:wortmannin|FD-Score:9.15|P-value:1.13E-13 ID:SGTC_1887|Compound:st057648|FD-Score:-3.88|P-value:8.66E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-5.05|P-value:2.32E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.90|P-value:9.26E-4 ID:SGTC_2029|Compound:5175081|FD-Score:4.44|P-value:1.92E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.57|P-value:1.30E-4 ID:SGTC_2077|Compound:5212015|FD-Score:9.55|P-value:9.80E-15 ID:SGTC_2090|Compound:5301565|FD-Score:4.48|P-value:1.72E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.95|P-value:7.96E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.51|P-value:1.56E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.13|P-value:4.86E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.60|P-value:1.18E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.81|P-value:5.18E-5 ID:SGTC_2275|Compound:7947463|FD-Score:7.35|P-value:1.99E-9 ID:SGTC_2291|Compound:7917025|FD-Score:3.89|P-value:9.35E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.34|P-value:2.62E-4 ID:SGTC_2322|Compound:7413319|FD-Score:13.40|P-value:2.00E-27 ID:SGTC_2389|Compound:9091542|FD-Score:9.50|P-value:1.30E-14 ID:SGTC_2459|Compound:5811157|FD-Score:4.39|P-value:2.23E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.33|P-value:2.70E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.07|P-value:5.79E-4 ID:SGTC_2481|Compound:5478948|FD-Score:6.24|P-value:3.00E-7 ID:SGTC_2482|Compound:5981709|FD-Score:4.63|P-value:1.08E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:19.10|P-value:4.31E-53 ID:SGTC_2526|Compound:patulin|FD-Score:4.92|P-value:4.22E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:3.90|P-value:9.26E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:7.47|P-value:1.11E-9 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.01|P-value:6.70E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:5.60|P-value:3.65E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.80|P-value:3.21E-18 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.19|P-value:4.10E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:9.13|P-value:1.27E-13 ID:SGTC_2743|Compound:auranofin|FD-Score:7.29|P-value:2.58E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.75|P-value:7.30E-5 ID:SGTC_2927|Compound:9011802|FD-Score:4.32|P-value:2.75E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.80|P-value:2.62E-8 ID:SGTC_3131|Compound:9127911|FD-Score:-4.72|P-value:7.04E-5 ID:SGTC_3133|Compound:9127736|FD-Score:-4.32|P-value:2.45E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.16|P-value:4.41E-4 ID:SGTC_3324|Compound:9138759|FD-Score:5.87|P-value:1.33E-6 ID:SGTC_3330|Compound:9141424|FD-Score:3.88|P-value:9.70E-4

Top fitness defect scores for YDL077C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2514 salinomycin 19.10 42.7 µM 4.31E-53 2 2.78E-81
2 SGTC_841 0251-0215 16.60 91.2 µM 8.20E-41 5 2.91E-62
3 SGTC_2322 7413319 13.40 38.2 µM 2.00E-27 ERAD & cell cycle 6 1.56E-41
4 SGTC_1687 st020778 13.20 55.4 µM 1.48E-26 27 3.45E-40
5 SGTC_1873 rapamycin 12.60 2.0 nM 3.41E-24 4 1.55E-36
6 SGTC_1495 4469-0029 11.70 237.0 µM 3.05E-21 9 5.72E-32
7 SGTC_351 caspofungin 10.90 25.0 nM 8.55E-19 cell wall 4 3.49E-28
8 SGTC_2679 rapamycin 10.80 1.0 nM 3.21E-18 19 2.70E-27
9 SGTC_1844 rapamycin 10.70 4.0 nM 4.72E-18 35 4.90E-27
10 SGTC_2077 5212015 9.55 200.0 µM 9.80E-15 8 6.53E-22
11 SGTC_2389 9091542 9.50 195.5 µM 1.30E-14 iron homeostasis 3 1.01E-21
12 SGTC_1877 wortmannin 9.15 25.0 µM 1.13E-13 19 2.84E-20
13 SGTC_2737 bleomycin 9.13 30.5 nM 1.27E-13 Golgi 14 3.40E-20
14 SGTC_117 lucigenin 9.09 22.1 µM 1.64E-13 redox potentiating 12 5.06E-20
15 SGTC_139 0099-0334 8.98 718.6 µM 3.10E-13 1 1.35E-19
16 SGTC_1658 st012944 8.82 20.0 µM 7.94E-13 iron homeostasis 7 5.79E-19
17 SGTC_459 nigericin 8.05 15.3 µM 5.74E-11 11 4.26E-16
18 SGTC_2597 laccaic acid a 7.47 100.0 µM 1.11E-9 4 4.07E-14
19 SGTC_2275 7947463 7.35 200.0 µM 1.99E-9 iron homeostasis 7 1.01E-13
20 SGTC_562 3772-2733 7.34 112.0 µM 2.05E-9 ERG2 24 1.05E-13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.623 0 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.611 0 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.426 3.17E-148 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.419 3.34E-143 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.374 7.35E-112 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.351 6.39E-98 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.331 1.14E-86 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.324 9.12E-83 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.313 3.87E-77 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.311 3.07E-76 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.289 1.40E-65 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.274 1.17E-58 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.269 1.57E-56 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.253 3.24E-50 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.253 4.81E-50 YGL012W ERG4 hom C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol