YDL083C / RPS16B

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL083C / RPS16B

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.45|P-value:2.88E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.48|P-value:8.28E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:-4.01|P-value:1.26E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:3.81|P-value:2.85E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.53|P-value:6.29E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.67|P-value:1.01E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.19|P-value:6.40E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.42|P-value:8.76E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:-5.06|P-value:1.95E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.96|P-value:1.74E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-4.05|P-value:1.07E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.96|P-value:1.71E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.73|P-value:7.76E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.64|P-value:1.14E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.43|P-value:8.54E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:4.14|P-value:9.05E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.72|P-value:3.87E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.64|P-value:4.96E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.94|P-value:1.82E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-4.00|P-value:1.28E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.86|P-value:4.65E-6 ID:SGTC_1739|Compound:st037196|FD-Score:-3.47|P-value:7.46E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.36|P-value:4.04E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.88|P-value:1.93E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.86|P-value:2.39E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.89|P-value:1.91E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-5.77|P-value:6.93E-8 ID:SGTC_2952|Compound:9073731|FD-Score:4.30|P-value:5.05E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.92|P-value:1.98E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.54|P-value:1.67E-5 ID:SGTC_2996|Compound:9066192|FD-Score:4.64|P-value:1.33E-5 ID:SGTC_2999|Compound:9069765|FD-Score:-6.59|P-value:9.06E-10 ID:SGTC_3244|Compound:9134489|FD-Score:3.57|P-value:6.20E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.67|P-value:4.57E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.45|P-value:2.88E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.48|P-value:8.28E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:-4.01|P-value:1.26E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:3.81|P-value:2.85E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.53|P-value:6.29E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.67|P-value:1.01E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.19|P-value:6.40E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.42|P-value:8.76E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:-5.06|P-value:1.95E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.96|P-value:1.74E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-4.05|P-value:1.07E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.96|P-value:1.71E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.73|P-value:7.76E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.64|P-value:1.14E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.43|P-value:8.54E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:4.14|P-value:9.05E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.72|P-value:3.87E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.64|P-value:4.96E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.94|P-value:1.82E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-4.00|P-value:1.28E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.86|P-value:4.65E-6 ID:SGTC_1739|Compound:st037196|FD-Score:-3.47|P-value:7.46E-4 ID:SGTC_2016|Compound:5121758|FD-Score:4.36|P-value:4.04E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.88|P-value:1.93E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.86|P-value:2.39E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.89|P-value:1.91E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-5.77|P-value:6.93E-8 ID:SGTC_2952|Compound:9073731|FD-Score:4.30|P-value:5.05E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.92|P-value:1.98E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.54|P-value:1.67E-5 ID:SGTC_2996|Compound:9066192|FD-Score:4.64|P-value:1.33E-5 ID:SGTC_2999|Compound:9069765|FD-Score:-6.59|P-value:9.06E-10 ID:SGTC_3244|Compound:9134489|FD-Score:3.57|P-value:6.20E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.67|P-value:4.57E-4

Top fitness defect scores for YDL083C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2996 9066192 4.64 71.4 µM 1.33E-5 14 1.74E-6
2 SGTC_15 0266-0086 4.45 27.1 µM 2.88E-5 endomembrane recycling 46 4.39E-6
3 SGTC_2016 5121758 4.36 140.0 µM 4.04E-5 redox potentiating 36 6.60E-6
4 SGTC_2952 9073731 4.30 13.8 µM 5.05E-5 tubulin folding & SWR complex 33 8.64E-6
5 SGTC_1288 0973-0013 4.14 65.5 µM 9.05E-5 12 1.74E-5
6 SGTC_1166 k206-0078 3.96 225.0 µM 1.71E-4 22 3.75E-5
7 SGTC_1052 3253-3998 3.96 198.0 µM 1.74E-4 16 3.82E-5
8 SGTC_1391 3442-0225 3.94 151.0 µM 1.82E-4 15 4.05E-5
9 SGTC_2978 9011784 3.92 43.0 µM 1.98E-4 29 4.47E-5
10 SGTC_2774 tamoxifen 3.86 10.9 µM 2.39E-4 16 5.60E-5
11 SGTC_195 4234-0266 3.81 16.6 µM 2.85E-4 38 6.94E-5
12 SGTC_1380 2291-0730 3.72 164.0 µM 3.87E-4 31 1.00E-4
13 SGTC_3245 9134821 3.67 49.5 µM 4.57E-4 28 1.22E-4
14 SGTC_1385 3091-4834 3.64 14.1 µM 4.96E-4 RPP1 & pyrimidine depletion 28 1.35E-4
15 SGTC_3244 9134489 3.57 49.5 µM 6.20E-4 27 1.76E-4
16 SGTC_146 3448-8650 3.48 53.1 µM 8.28E-4 NEO1-PIK1 175 2.50E-4
17 SGTC_2917 7979103 3.32 53.8 µM 0.00135 55 4.51E-4
18 SGTC_17 0960-0276 3.32 49.8 µM 0.00136 67 4.53E-4
19 SGTC_783 0848-0141 3.25 92.7 µM 0.00166 RSC & ERG11 30 5.75E-4
20 SGTC_1291 0993-0025 3.24 5.8 µM 0.00170 25 5.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 1.16E-13 YPL041C_p YPL041C_p hom Protein of unknown function involved in maintenance of proper telomere length
0.108 3.98E-10 YBR088C POL30 het Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
0.103 2.00E-9 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.103 2.46E-9 YER164W CHD1 hom Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
0.101 4.51E-9 YOL014W_p YOL014W_p hom Putative protein of unknown function
0.099 1.06E-8 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.093 6.10E-8 YDR244W PEX5 hom Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
0.089 2.70E-7 YHR016C YSC84 hom Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
0.088 3.02E-7 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.086 6.89E-7 YMR291W TDA1 hom Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.083 1.36E-6 YHR021C RPS27B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
0.081 2.35E-6 YBR133C HSL7 hom Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
0.081 2.72E-6 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.079 5.32E-6 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.078 6.22E-6 YLL013C PUF3 hom Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins