YDL088C / ASM4

FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL088C / ASM4

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.24|P-value:5.98E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.56|P-value:1.89E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:-3.31|P-value:6.40E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.58|P-value:2.49E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:3.20|P-value:6.72E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.09|P-value:9.97E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.43|P-value:4.20E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.14|P-value:8.40E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.18|P-value:2.30E-5 ID:SGTC_759|Compound:3486-0358|FD-Score:3.11|P-value:9.25E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.13|P-value:8.48E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.07|P-value:2.39E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.38|P-value:3.57E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.43|P-value:4.22E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.25|P-value:5.76E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.57|P-value:1.81E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.31|P-value:6.30E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.29|P-value:6.87E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:5.65|P-value:9.53E-9 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.96|P-value:5.57E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.07|P-value:2.43E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.32|P-value:4.51E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-3.45|P-value:3.86E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.83|P-value:6.45E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.28|P-value:5.10E-4 ID:SGTC_1642|Compound:st009582|FD-Score:4.74|P-value:1.19E-6 ID:SGTC_1670|Compound:st016595|FD-Score:3.47|P-value:2.58E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.81|P-value:8.21E-7 ID:SGTC_1824|Compound:st053723|FD-Score:3.14|P-value:8.32E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.93|P-value:6.23E-5 ID:SGTC_1896|Compound:st058441|FD-Score:5.13|P-value:1.63E-7 ID:SGTC_1916|Compound:st067606|FD-Score:3.12|P-value:9.01E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.04|P-value:2.73E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.11|P-value:9.17E-4 ID:SGTC_2426|Compound:5582046|FD-Score:-3.49|P-value:3.40E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.28|P-value:5.13E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.55|P-value:1.92E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.77|P-value:1.18E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.40|P-value:5.72E-6 ID:SGTC_2537|Compound:larixol|FD-Score:4.13|P-value:1.85E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.16|P-value:7.86E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.59|P-value:1.68E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.10|P-value:2.13E-5 ID:SGTC_2949|Compound:9072542|FD-Score:4.15|P-value:1.71E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-6.80|P-value:1.35E-11 ID:SGTC_2989|Compound:9073649|FD-Score:3.40|P-value:3.39E-4 ID:SGTC_3004|Compound:9076122|FD-Score:-3.30|P-value:6.56E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.93|P-value:4.42E-5 ID:SGTC_3029|Compound:9088683|FD-Score:3.83|P-value:6.47E-5 ID:SGTC_3030|Compound:9087619|FD-Score:4.03|P-value:2.87E-5 ID:SGTC_3201|Compound:9113999|FD-Score:-3.21|P-value:8.89E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.59|P-value:1.64E-4 ID:SGTC_3262|Compound:9137218|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.34|P-value:4.15E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.24|P-value:5.98E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.56|P-value:1.89E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:-3.31|P-value:6.40E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.58|P-value:2.49E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:3.20|P-value:6.72E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.09|P-value:9.97E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.43|P-value:4.20E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.14|P-value:8.40E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.18|P-value:2.30E-5 ID:SGTC_759|Compound:3486-0358|FD-Score:3.11|P-value:9.25E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.13|P-value:8.48E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.07|P-value:2.39E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.38|P-value:3.57E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.43|P-value:4.22E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.25|P-value:5.76E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.57|P-value:1.81E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.31|P-value:6.30E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.29|P-value:6.87E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:5.65|P-value:9.53E-9 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.96|P-value:5.57E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.07|P-value:2.43E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.32|P-value:4.51E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-3.45|P-value:3.86E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.83|P-value:6.45E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.28|P-value:5.10E-4 ID:SGTC_1642|Compound:st009582|FD-Score:4.74|P-value:1.19E-6 ID:SGTC_1670|Compound:st016595|FD-Score:3.47|P-value:2.58E-4 ID:SGTC_1808|Compound:st050063|FD-Score:4.81|P-value:8.21E-7 ID:SGTC_1824|Compound:st053723|FD-Score:3.14|P-value:8.32E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.93|P-value:6.23E-5 ID:SGTC_1896|Compound:st058441|FD-Score:5.13|P-value:1.63E-7 ID:SGTC_1916|Compound:st067606|FD-Score:3.12|P-value:9.01E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.04|P-value:2.73E-5 ID:SGTC_2364|Compound:9055074|FD-Score:3.11|P-value:9.17E-4 ID:SGTC_2426|Compound:5582046|FD-Score:-3.49|P-value:3.40E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.28|P-value:5.13E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.55|P-value:1.92E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.77|P-value:1.18E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.40|P-value:5.72E-6 ID:SGTC_2537|Compound:larixol|FD-Score:4.13|P-value:1.85E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.16|P-value:7.86E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.59|P-value:1.68E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.10|P-value:2.13E-5 ID:SGTC_2949|Compound:9072542|FD-Score:4.15|P-value:1.71E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-6.80|P-value:1.35E-11 ID:SGTC_2989|Compound:9073649|FD-Score:3.40|P-value:3.39E-4 ID:SGTC_3004|Compound:9076122|FD-Score:-3.30|P-value:6.56E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.93|P-value:4.42E-5 ID:SGTC_3029|Compound:9088683|FD-Score:3.83|P-value:6.47E-5 ID:SGTC_3030|Compound:9087619|FD-Score:4.03|P-value:2.87E-5 ID:SGTC_3201|Compound:9113999|FD-Score:-3.21|P-value:8.89E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.59|P-value:1.64E-4 ID:SGTC_3262|Compound:9137218|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.34|P-value:4.15E-4

Top fitness defect scores for YDL088C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1189 1431-2119 5.65 7.3 µM 9.53E-9 6 8.07E-9
2 SGTC_1896 st058441 5.13 89.0 µM 1.63E-7 3 1.45E-7
3 SGTC_1808 st050063 4.81 61.5 µM 8.21E-7 2 7.52E-7
4 SGTC_1642 st009582 4.74 77.7 µM 1.19E-6 RPP1 & pyrimidine depletion 6 1.09E-6
5 SGTC_114 0364-0001 4.58 490.3 µM 2.49E-6 25 2.33E-6
6 SGTC_2533 agelasine 4.40 13.7 µM 5.72E-6 ERG2 40 5.41E-6
7 SGTC_2949 9072542 4.15 2.2 µM 1.71E-5 TSC3-RPN4 31 1.65E-5
8 SGTC_2537 larixol 4.13 68.9 µM 1.85E-5 20 1.78E-5
9 SGTC_2930 9013230 4.10 14.9 µM 2.13E-5 heme biosynthesis & mitochondrial translocase 29 2.05E-5
10 SGTC_1087 pterostilbene 4.07 9.1 µM 2.39E-5 22 2.31E-5
11 SGTC_1205 4161-2785 4.07 76.0 µM 2.43E-5 25 2.35E-5
12 SGTC_2239 6685329 4.04 187.6 µM 2.73E-5 26 2.65E-5
13 SGTC_3030 9087619 4.03 49.5 µM 2.87E-5 8 2.78E-5
14 SGTC_3006 9075424 3.93 71.4 µM 4.42E-5 11 4.31E-5
15 SGTC_1396 3511-0007 3.83 98.6 µM 6.45E-5 mitochondrial processes 14 6.33E-5
16 SGTC_3029 9088683 3.83 49.5 µM 6.47E-5 25 6.35E-5
17 SGTC_3209 9113616 3.59 49.5 µM 1.64E-4 39 1.63E-4
18 SGTC_3262 9137218 3.59 49.5 µM 1.67E-4 3 1.66E-4
19 SGTC_2688 kanamycin b 3.59 96.7 µM 1.68E-4 calcium & mitochondrial duress 428 1.67E-4
20 SGTC_1152 3909-8331 3.57 15.9 µM 1.81E-4 20 1.80E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 1.02E-12 YGL137W SEC27 het Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
0.121 1.97E-12 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.108 4.21E-10 YJL156C SSY5 het Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
0.096 2.61E-8 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.091 1.39E-7 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.090 1.80E-7 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.089 2.25E-7 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.087 4.69E-7 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.086 6.16E-7 YGL066W SGF73 hom SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.084 9.98E-7 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.084 1.16E-6 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.082 1.83E-6 YNL205C_d YNL205C_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.37E-6 YML077W BET5 het Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
0.081 2.70E-6 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.081 2.95E-6 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome