YDL095W / PMT1

Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL095W / PMT1

Click on Significant Values for Screen Details ID:SGTC_98|Compound:0074-0023|FD-Score:3.81|P-value:4.11E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:9.16|P-value:3.90E-16 ID:SGTC_143|Compound:4032-1194|FD-Score:11.70|P-value:4.06E-25 ID:SGTC_306|Compound:1672-3436|FD-Score:3.88|P-value:3.27E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.54|P-value:9.51E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:3.55|P-value:9.05E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.79|P-value:4.30E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-4.06|P-value:1.70E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:4.55|P-value:3.16E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.53|P-value:9.74E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:4.29|P-value:8.07E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.35|P-value:1.14E-8 ID:SGTC_830|Compound:4076-0286|FD-Score:3.74|P-value:5.16E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:3.63|P-value:7.09E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.91|P-value:7.96E-6 ID:SGTC_1163|Compound:k060-0048|FD-Score:4.37|P-value:6.23E-5 ID:SGTC_1614|Compound:st002383|FD-Score:4.10|P-value:1.59E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-3.85|P-value:3.36E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.70|P-value:5.75E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.84|P-value:3.73E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-5.74|P-value:2.02E-7 ID:SGTC_2708|Compound:st078559|FD-Score:-3.81|P-value:3.86E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.59|P-value:8.11E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.46|P-value:4.12E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.71|P-value:5.54E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.81|P-value:4.11E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:9.16|P-value:3.90E-16 ID:SGTC_143|Compound:4032-1194|FD-Score:11.70|P-value:4.06E-25 ID:SGTC_306|Compound:1672-3436|FD-Score:3.88|P-value:3.27E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.54|P-value:9.51E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:3.55|P-value:9.05E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.79|P-value:4.30E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-4.06|P-value:1.70E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:4.55|P-value:3.16E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.53|P-value:9.74E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:4.29|P-value:8.07E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.35|P-value:1.14E-8 ID:SGTC_830|Compound:4076-0286|FD-Score:3.74|P-value:5.16E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:3.63|P-value:7.09E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.91|P-value:7.96E-6 ID:SGTC_1163|Compound:k060-0048|FD-Score:4.37|P-value:6.23E-5 ID:SGTC_1614|Compound:st002383|FD-Score:4.10|P-value:1.59E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-3.85|P-value:3.36E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.70|P-value:5.75E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.84|P-value:3.73E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-5.74|P-value:2.02E-7 ID:SGTC_2708|Compound:st078559|FD-Score:-3.81|P-value:3.86E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.59|P-value:8.11E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.46|P-value:4.12E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.71|P-value:5.54E-4

Top fitness defect scores for YDL095W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_143 4032-1194 11.70 15.7 µM 4.06E-25 3 7.31E-32
2 SGTC_117 lucigenin 9.16 22.1 µM 3.90E-16 redox potentiating 10 2.65E-20
3 SGTC_800 3448-7362 6.35 155.0 µM 1.14E-8 translation 37 1.04E-10
4 SGTC_1028 k015-0029 4.91 4.8 µM 7.96E-6 8 4.56E-7
5 SGTC_572 0960-0087 4.55 17.7 µM 3.16E-5 17 2.66E-6
6 SGTC_1163 k060-0048 4.37 49.9 µM 6.23E-5 14 6.32E-6
7 SGTC_663 1486-0733 4.29 13.9 µM 8.07E-5 cell wall signaling 44 8.80E-6
8 SGTC_1614 st002383 4.10 48.6 µM 1.59E-4 cell wall signaling 14 2.09E-5
9 SGTC_306 1672-3436 3.88 11.6 µM 3.27E-4 Golgi 15 5.23E-5
10 SGTC_2399 5884133 3.84 200.0 µM 3.73E-4 cell wall signaling 78 6.19E-5
11 SGTC_98 0074-0023 3.81 107.7 µM 4.11E-4 Golgi 43 7.00E-5
12 SGTC_531 3788-1495 3.79 174.0 µM 4.30E-4 unfolded protein response 20 7.41E-5
13 SGTC_830 4076-0286 3.74 57.8 µM 5.16E-4 translation 71 9.35E-5
14 SGTC_3211 9128781 3.71 49.5 µM 5.54E-4 73 1.02E-4
15 SGTC_2007 4011539 3.70 71.8 µM 5.75E-4 cell wall signaling 72 1.07E-4
16 SGTC_906 1165-0455 3.63 526.0 µM 7.09E-4 18 1.40E-4
17 SGTC_2784 7567673 3.59 72.7 µM 8.11E-4 iron homeostasis 42 1.66E-4
18 SGTC_437 s-farnesyl-l-cysteine methyl ester 3.55 6.7 µM 9.05E-4 19 1.91E-4
19 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 3.54 8.0 µM 9.51E-4 40 2.04E-4
20 SGTC_625 k213-0058 3.53 121.0 µM 9.74E-4 translation 141 2.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.395 4.03E-125 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.275 2.72E-59 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.209 1.86E-34 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.207 9.75E-34 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.184 4.87E-27 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.175 1.99E-24 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.167 1.85E-22 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.144 5.81E-17 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.138 1.00E-15 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.132 1.54E-14 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.127 1.45E-13 YDR245W MNN10 hom Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
0.125 3.34E-13 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.125 3.53E-13 YOR067C ALG8 hom Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.125 3.82E-13 YEL003W GIM4 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.124 6.42E-13 YJL139C YUR1 hom Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication