YDL097C / RPN6

Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL097C / RPN6

Click on Significant Values for Screen Details ID:SGTC_81|Compound:0411-0046|FD-Score:-3.97|P-value:8.14E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:4.89|P-value:5.24E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.02|P-value:7.04E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:5.98|P-value:1.07E-6 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.01|P-value:7.49E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.78|P-value:7.45E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:5.14|P-value:2.27E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.45|P-value:2.09E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:6.15|P-value:5.31E-7 ID:SGTC_790|Compound:1575-0012|FD-Score:4.73|P-value:8.95E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:4.55|P-value:1.53E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.43|P-value:2.21E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.63|P-value:1.22E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.58|P-value:1.39E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.13|P-value:5.27E-4 ID:SGTC_1770|Compound:st043884|FD-Score:4.55|P-value:1.55E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.48|P-value:1.90E-4 ID:SGTC_2004|Compound:4023728|FD-Score:5.12|P-value:2.46E-5 ID:SGTC_2071|Compound:5230951|FD-Score:5.79|P-value:2.21E-6 ID:SGTC_2124|Compound:5246320|FD-Score:5.69|P-value:3.20E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.24|P-value:3.86E-4 ID:SGTC_2158|Compound:5657123|FD-Score:4.72|P-value:9.10E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.76|P-value:8.05E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.53|P-value:1.65E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.92|P-value:9.55E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.52|P-value:1.72E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.92|P-value:9.56E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.12|P-value:5.52E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-4.83|P-value:6.26E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-4.34|P-value:2.87E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.48|P-value:1.94E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:5.36|P-value:1.06E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-5.05|P-value:3.05E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.54|P-value:1.54E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-6.66|P-value:6.21E-8 ID:SGTC_2785|Compound:7756463|FD-Score:6.08|P-value:7.24E-7 ID:SGTC_2790|Compound:5423970|FD-Score:6.00|P-value:9.86E-7 ID:SGTC_2837|Compound:9008476|FD-Score:-3.93|P-value:9.05E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.06|P-value:6.47E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.23|P-value:3.84E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.21|P-value:4.22E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.12|P-value:2.42E-5 ID:SGTC_3030|Compound:9087619|FD-Score:5.34|P-value:1.14E-5 ID:SGTC_3038|Compound:9091290|FD-Score:-3.96|P-value:8.38E-4 ID:SGTC_3081|Compound:9117243|FD-Score:4.35|P-value:2.81E-4 ID:SGTC_3142|Compound:9086498|FD-Score:6.44|P-value:1.62E-7 ID:SGTC_3178|Compound:9107506|FD-Score:5.98|P-value:1.07E-6 ID:SGTC_3200|Compound:9114385|FD-Score:4.54|P-value:1.59E-4 ID:SGTC_3317|Compound:9138495|FD-Score:5.70|P-value:3.15E-6 ID:SGTC_3318|Compound:9139014|FD-Score:4.36|P-value:2.71E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:-3.97|P-value:8.14E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:4.89|P-value:5.24E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.02|P-value:7.04E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:5.98|P-value:1.07E-6 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.01|P-value:7.49E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.78|P-value:7.45E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:5.14|P-value:2.27E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.45|P-value:2.09E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:6.15|P-value:5.31E-7 ID:SGTC_790|Compound:1575-0012|FD-Score:4.73|P-value:8.95E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:4.55|P-value:1.53E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.43|P-value:2.21E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.63|P-value:1.22E-4 ID:SGTC_1638|Compound:st008370|FD-Score:4.58|P-value:1.39E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.13|P-value:5.27E-4 ID:SGTC_1770|Compound:st043884|FD-Score:4.55|P-value:1.55E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.48|P-value:1.90E-4 ID:SGTC_2004|Compound:4023728|FD-Score:5.12|P-value:2.46E-5 ID:SGTC_2071|Compound:5230951|FD-Score:5.79|P-value:2.21E-6 ID:SGTC_2124|Compound:5246320|FD-Score:5.69|P-value:3.20E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.24|P-value:3.86E-4 ID:SGTC_2158|Compound:5657123|FD-Score:4.72|P-value:9.10E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.76|P-value:8.05E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.53|P-value:1.65E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.92|P-value:9.55E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.52|P-value:1.72E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.92|P-value:9.56E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.12|P-value:5.52E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-4.83|P-value:6.26E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-4.34|P-value:2.87E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.48|P-value:1.94E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:5.36|P-value:1.06E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-5.05|P-value:3.05E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.54|P-value:1.54E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-6.66|P-value:6.21E-8 ID:SGTC_2785|Compound:7756463|FD-Score:6.08|P-value:7.24E-7 ID:SGTC_2790|Compound:5423970|FD-Score:6.00|P-value:9.86E-7 ID:SGTC_2837|Compound:9008476|FD-Score:-3.93|P-value:9.05E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.06|P-value:6.47E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.23|P-value:3.84E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.21|P-value:4.22E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.12|P-value:2.42E-5 ID:SGTC_3030|Compound:9087619|FD-Score:5.34|P-value:1.14E-5 ID:SGTC_3038|Compound:9091290|FD-Score:-3.96|P-value:8.38E-4 ID:SGTC_3081|Compound:9117243|FD-Score:4.35|P-value:2.81E-4 ID:SGTC_3142|Compound:9086498|FD-Score:6.44|P-value:1.62E-7 ID:SGTC_3178|Compound:9107506|FD-Score:5.98|P-value:1.07E-6 ID:SGTC_3200|Compound:9114385|FD-Score:4.54|P-value:1.59E-4 ID:SGTC_3317|Compound:9138495|FD-Score:5.70|P-value:3.15E-6 ID:SGTC_3318|Compound:9139014|FD-Score:4.36|P-value:2.71E-4

Top fitness defect scores for YDL097C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3142 9086498 6.44 49.5 µM 1.62E-7 iron homeostasis 21 5.85E-11 0.12
2 SGTC_695 3505-6186 6.15 243.0 µM 5.31E-7 1 3.77E-10 1.14
3 SGTC_2785 7756463 6.08 72.7 µM 7.24E-7 2 6.13E-10 0.47
4 SGTC_2790 5423970 6.00 71.4 µM 9.86E-7 endomembrane recycling 4 9.94E-10 0.01
5 SGTC_3178 9107506 5.98 49.5 µM 1.07E-6 1 1.12E-9 0.56
6 SGTC_413 propylparaben 5.98 100.0 µM 1.07E-6 amide catabolism 1 1.13E-9 2.12
7 SGTC_2071 5230951 5.79 39.9 µM 2.21E-6 TSC3-RPN4 3 3.53E-9 0.94
8 SGTC_3317 9138495 5.70 8.8 µM 3.15E-6 6 6.12E-9 0.08
9 SGTC_2124 5246320 5.69 200.0 µM 3.20E-6 heme biosynthesis & mitochondrial translocase 17 6.28E-9 0.09
10 SGTC_2617 dihydrotanshinone i 5.36 20.8 µM 1.06E-5 copper-dependent oxidative stress 12 4.09E-8 0.03
11 SGTC_3030 9087619 5.34 49.5 µM 1.14E-5 1 4.57E-8 0.53
12 SGTC_553 k007-0256 5.14 45.7 µM 2.27E-5 heme biosynthesis & mitochondrial translocase 14 1.35E-7 0.06
13 SGTC_2004 4023728 5.12 41.9 µM 2.46E-5 17 1.52E-7 0.41
14 SGTC_211 3455-0679 4.89 143.5 µM 5.24E-5 6 4.95E-7 0.90
15 SGTC_546 peri-Naphthalide 4.78 147.0 µM 7.45E-5 DNA damage response 15 8.57E-7 0.15
16 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 4.76 49.5 µM 8.05E-5 13 9.68E-7 0.00
17 SGTC_790 1575-0012 4.73 79.1 µM 8.95E-5 TSC3-RPN4 5 1.14E-6 1.25
18 SGTC_2158 5657123 4.72 3.6 µM 9.10E-5 8 1.17E-6 0.07
19 SGTC_1524 corynanthine 4.63 62.1 µM 1.22E-4 3 1.84E-6 0.09
20 SGTC_1638 st008370 4.58 60.6 µM 1.39E-4 ergosterol biosynthesis 11 2.27E-6 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 5.39E-60 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.254 2.26E-50 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.253 6.29E-50 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.224 4.33E-39 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.192 4.60E-29 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.185 2.56E-27 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.183 1.63E-26 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.176 1.06E-24 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.141 2.71E-16 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.131 2.48E-14 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.126 2.08E-13 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.125 4.04E-13 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.115 2.18E-11 YOR261C RPN8 het Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
0.110 2.02E-10 YBR025C OLA1 hom P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
0.109 2.67E-10 YMR290W-A_d YMR290W-A_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase