YDL099W / BUG1

Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL099W / BUG1

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:3.76|P-value:7.01E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:2.76|P-value:7.28E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.28|P-value:7.78E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.69|P-value:1.04E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:2.72|P-value:8.55E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.78|P-value:6.56E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:2.89|P-value:4.20E-4 ID:SGTC_500|Compound:h-89|FD-Score:2.90|P-value:4.13E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.28|P-value:7.49E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:-2.71|P-value:9.29E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-2.92|P-value:3.85E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.36|P-value:5.28E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.06|P-value:2.09E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:5.24|P-value:7.28E-10 ID:SGTC_684|Compound:1140-0066|FD-Score:2.80|P-value:6.12E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:2.72|P-value:8.32E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:15.80|P-value:2.36E-74 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.16|P-value:8.10E-7 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.59|P-value:1.74E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.72|P-value:9.29E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:-3.15|P-value:1.46E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.15|P-value:8.59E-7 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.66|P-value:1.23E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-2.72|P-value:8.71E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.06|P-value:2.20E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-2.75|P-value:7.78E-4 ID:SGTC_1769|Compound:st044406|FD-Score:-2.84|P-value:5.47E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.33|P-value:6.24E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.38|P-value:5.08E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-4.09|P-value:1.27E-6 ID:SGTC_2346|Compound:9031566|FD-Score:2.74|P-value:7.75E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.62|P-value:1.46E-5 ID:SGTC_2432|Compound:5630864|FD-Score:2.77|P-value:6.97E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.06|P-value:2.10E-4 ID:SGTC_2624|Compound:nerol|FD-Score:2.83|P-value:5.34E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.05|P-value:2.21E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:-2.84|P-value:5.37E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.06|P-value:2.06E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.20|P-value:1.13E-4 ID:SGTC_3046|Compound:9092212|FD-Score:-3.05|P-value:2.28E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.82|P-value:5.31E-6 ID:SGTC_3350|Compound:9150499|FD-Score:-2.73|P-value:8.48E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:3.76|P-value:7.01E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:2.76|P-value:7.28E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.28|P-value:7.78E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.69|P-value:1.04E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:2.72|P-value:8.55E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.78|P-value:6.56E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:2.89|P-value:4.20E-4 ID:SGTC_500|Compound:h-89|FD-Score:2.90|P-value:4.13E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.28|P-value:7.49E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:-2.71|P-value:9.29E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-2.92|P-value:3.85E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.36|P-value:5.28E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.06|P-value:2.09E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:5.24|P-value:7.28E-10 ID:SGTC_684|Compound:1140-0066|FD-Score:2.80|P-value:6.12E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:2.72|P-value:8.32E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:15.80|P-value:2.36E-74 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.16|P-value:8.10E-7 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.59|P-value:1.74E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.72|P-value:9.29E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:-3.15|P-value:1.46E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.15|P-value:8.59E-7 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.66|P-value:1.23E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-2.72|P-value:8.71E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.06|P-value:2.20E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-2.75|P-value:7.78E-4 ID:SGTC_1769|Compound:st044406|FD-Score:-2.84|P-value:5.47E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.33|P-value:6.24E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.38|P-value:5.08E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-4.09|P-value:1.27E-6 ID:SGTC_2346|Compound:9031566|FD-Score:2.74|P-value:7.75E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.62|P-value:1.46E-5 ID:SGTC_2432|Compound:5630864|FD-Score:2.77|P-value:6.97E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.06|P-value:2.10E-4 ID:SGTC_2624|Compound:nerol|FD-Score:2.83|P-value:5.34E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.05|P-value:2.21E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:-2.84|P-value:5.37E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.06|P-value:2.06E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.20|P-value:1.13E-4 ID:SGTC_3046|Compound:9092212|FD-Score:-3.05|P-value:2.28E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.82|P-value:5.31E-6 ID:SGTC_3350|Compound:9150499|FD-Score:-2.73|P-value:8.48E-4

Top fitness defect scores for YDL099W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 15.80 1.8 µM 2.36E-74 4 1.76E-56
2 SGTC_638 3970-0837 5.24 4.1 µM 7.28E-10 16 7.86E-8
3 SGTC_1174 0739-0097 4.16 51.1 µM 8.10E-7 redox potentiating 23 1.62E-5
4 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 4.15 85.4 µM 8.59E-7 20 1.69E-5
5 SGTC_3058 9093861 3.82 49.5 µM 5.31E-6 21 6.77E-5
6 SGTC_1 0258-0098 3.76 57.4 µM 7.01E-6 heme biosynthesis & mitochondrial translocase 10 8.36E-5
7 SGTC_159 3970-0959 3.69 50.0 µM 1.04E-5 93 1.13E-4
8 SGTC_1572 epiandrosterone 3.66 68.9 µM 1.23E-5 37 1.28E-4
9 SGTC_2425 5628681 3.62 7.8 µM 1.46E-5 36 1.47E-4
10 SGTC_1376 2910-0861 3.59 41.3 µM 1.74E-5 30 1.67E-4
11 SGTC_626 0214-0009 3.36 33.0 µM 5.28E-5 copper-dependent oxidative stress 39 3.91E-4
12 SGTC_519 k048-0011 3.28 62.8 µM 7.49E-5 45 5.10E-4
13 SGTC_70 0141-0289 3.28 43.3 µM 7.78E-5 mitochondrial stress 240 5.25E-4
14 SGTC_2925 9010536 3.20 32.5 µM 1.13E-4 azole & statin 83 6.98E-4
15 SGTC_2640 pentamidine 3.06 10.4 µM 2.06E-4 mitochondrial processes 64 0.00111
16 SGTC_629 3448-6466 3.06 76.7 µM 2.09E-4 58 0.00112
17 SGTC_2499 avocadenofuran 3.06 51.5 µM 2.10E-4 84 0.00112
18 SGTC_500 h-89 2.90 112.0 µM 4.13E-4 cell wall signaling 77 0.00189
19 SGTC_452 diindolylmethane 2.89 8.0 µM 4.20E-4 RNA processing & uracil transport 43 0.00191
20 SGTC_2624 nerol 2.83 100.0 µM 5.34E-4 55 0.00230

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.652 0 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.443 2.68E-161 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.407 5.40E-134 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.404 1.33E-131 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.398 1.55E-127 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.396 3.60E-126 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.393 1.34E-124 YJL149W DAS1 hom Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
0.358 1.29E-101 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.353 8.40E-99 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.351 5.43E-98 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.340 1.43E-91 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.332 2.57E-87 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.319 3.79E-80 YJR014W TMA22 hom Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
0.311 5.26E-76 YDL177C_p YDL177C_p hom Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
0.305 2.24E-73 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication