YDL100C / GET3

Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL100C / GET3

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:6.39|P-value:7.00E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.36|P-value:1.19E-10 ID:SGTC_70|Compound:0141-0289|FD-Score:5.70|P-value:8.49E-6 ID:SGTC_81|Compound:0411-0046|FD-Score:5.17|P-value:4.83E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:9.08|P-value:3.51E-12 ID:SGTC_108|Compound:0509-0257|FD-Score:5.21|P-value:4.20E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:8.13|P-value:4.06E-10 ID:SGTC_115|Compound:0527-0192|FD-Score:7.68|P-value:3.41E-9 ID:SGTC_119|Compound:0121-0043|FD-Score:4.26|P-value:6.56E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.41|P-value:4.47E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:7.57|P-value:5.49E-9 ID:SGTC_208|Compound:k072-0098|FD-Score:5.58|P-value:1.26E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.33|P-value:5.50E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:5.00|P-value:8.20E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.72|P-value:1.95E-7 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.77|P-value:1.50E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:6.22|P-value:1.33E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.27|P-value:5.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:6.10|P-value:2.09E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:7.67|P-value:3.57E-9 ID:SGTC_373|Compound:0844-0104|FD-Score:11.80|P-value:3.27E-19 ID:SGTC_384|Compound:k007-0144|FD-Score:13.10|P-value:2.96E-23 ID:SGTC_426|Compound:0988-0037|FD-Score:6.35|P-value:8.18E-7 ID:SGTC_548|Compound:1436-0017|FD-Score:5.11|P-value:5.78E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:8.76|P-value:1.80E-11 ID:SGTC_660|Compound:4031-1742|FD-Score:4.24|P-value:6.99E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.62|P-value:2.50E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:5.64|P-value:1.05E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:10.70|P-value:3.41E-16 ID:SGTC_768|Compound:0384-0033|FD-Score:6.48|P-value:5.03E-7 ID:SGTC_805|Compound:3852-0218|FD-Score:7.58|P-value:5.19E-9 ID:SGTC_866|Compound:0495-0101|FD-Score:-4.60|P-value:2.38E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:13.60|P-value:4.76E-25 ID:SGTC_926|Compound:2268-1401|FD-Score:4.85|P-value:1.27E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.43|P-value:4.20E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:4.14|P-value:8.98E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.48|P-value:4.93E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:5.33|P-value:2.86E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:13.10|P-value:2.15E-23 ID:SGTC_1165|Compound:k072-0275|FD-Score:5.06|P-value:6.72E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.30|P-value:5.98E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:9.03|P-value:4.49E-12 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.12|P-value:8.82E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.62|P-value:2.44E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.14|P-value:9.14E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-4.25|P-value:6.33E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-5.36|P-value:2.36E-5 ID:SGTC_1731|Compound:st035964|FD-Score:8.28|P-value:2.02E-10 ID:SGTC_1736|Compound:st036774|FD-Score:4.97|P-value:8.99E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.22|P-value:2.48E-8 ID:SGTC_1752|Compound:st044819|FD-Score:4.58|P-value:2.77E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.70|P-value:7.75E-6 ID:SGTC_1797|Compound:st048695|FD-Score:4.14|P-value:9.13E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-4.64|P-value:2.16E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-5.01|P-value:7.30E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:8.39|P-value:1.19E-10 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.86|P-value:1.25E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:-4.24|P-value:6.49E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-4.46|P-value:3.56E-4 ID:SGTC_1953|Compound:st076800|FD-Score:5.36|P-value:2.62E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.96|P-value:9.04E-5 ID:SGTC_2067|Compound:5224629|FD-Score:5.35|P-value:2.74E-5 ID:SGTC_2149|Compound:5554786|FD-Score:4.96|P-value:9.15E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.87|P-value:1.19E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.14|P-value:8.94E-4 ID:SGTC_2291|Compound:7917025|FD-Score:8.33|P-value:1.60E-10 ID:SGTC_2315|Compound:7733312|FD-Score:4.47|P-value:3.74E-4 ID:SGTC_2322|Compound:7413319|FD-Score:10.80|P-value:2.01E-16 ID:SGTC_2385|Compound:9074692|FD-Score:6.36|P-value:8.01E-7 ID:SGTC_2426|Compound:5582046|FD-Score:4.76|P-value:1.65E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:5.50|P-value:1.68E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:5.82|P-value:5.71E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.12|P-value:8.72E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.18|P-value:7.61E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:5.83|P-value:5.40E-6 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.31|P-value:3.07E-5 ID:SGTC_2784|Compound:7567673|FD-Score:4.77|P-value:1.62E-4 ID:SGTC_2938|Compound:9059746|FD-Score:5.15|P-value:5.12E-5 ID:SGTC_2964|Compound:9083345|FD-Score:7.32|P-value:1.60E-8 ID:SGTC_3028|Compound:9087778|FD-Score:4.62|P-value:2.50E-4 ID:SGTC_3081|Compound:9117243|FD-Score:4.24|P-value:6.89E-4 ID:SGTC_3090|Compound:9117696|FD-Score:4.58|P-value:2.80E-4 ID:SGTC_3094|Compound:9117742|FD-Score:7.23|P-value:2.43E-8 ID:SGTC_3260|Compound:9137109|FD-Score:6.30|P-value:1.00E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-6.33|P-value:7.89E-7 ID:SGTC_3275|Compound:9138069|FD-Score:-4.67|P-value:1.99E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-5.82|P-value:5.08E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:6.39|P-value:7.00E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.36|P-value:1.19E-10 ID:SGTC_70|Compound:0141-0289|FD-Score:5.70|P-value:8.49E-6 ID:SGTC_81|Compound:0411-0046|FD-Score:5.17|P-value:4.83E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:9.08|P-value:3.51E-12 ID:SGTC_108|Compound:0509-0257|FD-Score:5.21|P-value:4.20E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:8.13|P-value:4.06E-10 ID:SGTC_115|Compound:0527-0192|FD-Score:7.68|P-value:3.41E-9 ID:SGTC_119|Compound:0121-0043|FD-Score:4.26|P-value:6.56E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.41|P-value:4.47E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:7.57|P-value:5.49E-9 ID:SGTC_208|Compound:k072-0098|FD-Score:5.58|P-value:1.26E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.33|P-value:5.50E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:5.00|P-value:8.20E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.72|P-value:1.95E-7 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.77|P-value:1.50E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:6.22|P-value:1.33E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.27|P-value:5.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:6.10|P-value:2.09E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:7.67|P-value:3.57E-9 ID:SGTC_373|Compound:0844-0104|FD-Score:11.80|P-value:3.27E-19 ID:SGTC_384|Compound:k007-0144|FD-Score:13.10|P-value:2.96E-23 ID:SGTC_426|Compound:0988-0037|FD-Score:6.35|P-value:8.18E-7 ID:SGTC_548|Compound:1436-0017|FD-Score:5.11|P-value:5.78E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:8.76|P-value:1.80E-11 ID:SGTC_660|Compound:4031-1742|FD-Score:4.24|P-value:6.99E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.62|P-value:2.50E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:5.64|P-value:1.05E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:10.70|P-value:3.41E-16 ID:SGTC_768|Compound:0384-0033|FD-Score:6.48|P-value:5.03E-7 ID:SGTC_805|Compound:3852-0218|FD-Score:7.58|P-value:5.19E-9 ID:SGTC_866|Compound:0495-0101|FD-Score:-4.60|P-value:2.38E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:13.60|P-value:4.76E-25 ID:SGTC_926|Compound:2268-1401|FD-Score:4.85|P-value:1.27E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.43|P-value:4.20E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:4.14|P-value:8.98E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.48|P-value:4.93E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:5.33|P-value:2.86E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:13.10|P-value:2.15E-23 ID:SGTC_1165|Compound:k072-0275|FD-Score:5.06|P-value:6.72E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.30|P-value:5.98E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:9.03|P-value:4.49E-12 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.12|P-value:8.82E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.62|P-value:2.44E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.14|P-value:9.14E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-4.25|P-value:6.33E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-5.36|P-value:2.36E-5 ID:SGTC_1731|Compound:st035964|FD-Score:8.28|P-value:2.02E-10 ID:SGTC_1736|Compound:st036774|FD-Score:4.97|P-value:8.99E-5 ID:SGTC_1750|Compound:st081859|FD-Score:7.22|P-value:2.48E-8 ID:SGTC_1752|Compound:st044819|FD-Score:4.58|P-value:2.77E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.70|P-value:7.75E-6 ID:SGTC_1797|Compound:st048695|FD-Score:4.14|P-value:9.13E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-4.64|P-value:2.16E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-5.01|P-value:7.30E-5 ID:SGTC_1872|Compound:amphotericin b|FD-Score:8.39|P-value:1.19E-10 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.86|P-value:1.25E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:-4.24|P-value:6.49E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-4.46|P-value:3.56E-4 ID:SGTC_1953|Compound:st076800|FD-Score:5.36|P-value:2.62E-5 ID:SGTC_2050|Compound:5263056|FD-Score:4.96|P-value:9.04E-5 ID:SGTC_2067|Compound:5224629|FD-Score:5.35|P-value:2.74E-5 ID:SGTC_2149|Compound:5554786|FD-Score:4.96|P-value:9.15E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.87|P-value:1.19E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.14|P-value:8.94E-4 ID:SGTC_2291|Compound:7917025|FD-Score:8.33|P-value:1.60E-10 ID:SGTC_2315|Compound:7733312|FD-Score:4.47|P-value:3.74E-4 ID:SGTC_2322|Compound:7413319|FD-Score:10.80|P-value:2.01E-16 ID:SGTC_2385|Compound:9074692|FD-Score:6.36|P-value:8.01E-7 ID:SGTC_2426|Compound:5582046|FD-Score:4.76|P-value:1.65E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:5.50|P-value:1.68E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:5.82|P-value:5.71E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.12|P-value:8.72E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.18|P-value:7.61E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:5.83|P-value:5.40E-6 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.31|P-value:3.07E-5 ID:SGTC_2784|Compound:7567673|FD-Score:4.77|P-value:1.62E-4 ID:SGTC_2938|Compound:9059746|FD-Score:5.15|P-value:5.12E-5 ID:SGTC_2964|Compound:9083345|FD-Score:7.32|P-value:1.60E-8 ID:SGTC_3028|Compound:9087778|FD-Score:4.62|P-value:2.50E-4 ID:SGTC_3081|Compound:9117243|FD-Score:4.24|P-value:6.89E-4 ID:SGTC_3090|Compound:9117696|FD-Score:4.58|P-value:2.80E-4 ID:SGTC_3094|Compound:9117742|FD-Score:7.23|P-value:2.43E-8 ID:SGTC_3260|Compound:9137109|FD-Score:6.30|P-value:1.00E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-6.33|P-value:7.89E-7 ID:SGTC_3275|Compound:9138069|FD-Score:-4.67|P-value:1.99E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-5.82|P-value:5.08E-6

Top fitness defect scores for YDL100C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_922 1866-0035 13.60 480.0 µM 4.76E-25 excess fatty acid 2 2.24E-42
2 SGTC_1161 k007-0610 13.10 110.0 µM 2.15E-23 ERAD & cell cycle 8 1.71E-39
3 SGTC_384 k007-0144 13.10 5.2 µM 2.96E-23 ERAD & cell cycle 4 2.99E-39
4 SGTC_373 0844-0104 11.80 25.9 µM 3.27E-19 ERAD & cell cycle 5 3.28E-32
5 SGTC_2322 7413319 10.80 38.2 µM 2.01E-16 ERAD & cell cycle 15 2.33E-27
6 SGTC_733 4069-0007 10.70 56.1 µM 3.41E-16 ERAD & cell cycle 10 5.82E-27
7 SGTC_103 0844-0104 9.08 51.8 µM 3.51E-12 ERAD & cell cycle 37 5.46E-20
8 SGTC_1255 0566-0045 9.03 221.0 µM 4.49E-12 excess fatty acid 3 8.39E-20
9 SGTC_568 1193-0097 8.76 7.5 µM 1.80E-11 ERAD & cell cycle 31 9.34E-19
10 SGTC_1872 amphotericin b 8.39 148.0 nM 1.19E-10 RNA pol III & RNase P/MRP 5 2.46E-17
11 SGTC_2291 7917025 8.33 104.3 µM 1.60E-10 iron homeostasis 15 4.13E-17
12 SGTC_1731 st035964 8.28 63.0 µM 2.02E-10 1 6.18E-17
13 SGTC_114 0364-0001 8.13 490.3 µM 4.06E-10 3 2.06E-16
14 SGTC_115 0527-0192 7.68 112.0 µM 3.41E-9 10 8.24E-15
15 SGTC_363 0455-0021 7.67 3.1 µM 3.57E-9 azole & statin 15 8.91E-15
16 SGTC_805 3852-0218 7.58 479.0 µM 5.19E-9 ERAD & cell cycle 13 1.71E-14
17 SGTC_188 3229-0966 7.57 278.5 µM 5.49E-9 7 1.88E-14
18 SGTC_2964 9083345 7.32 53.4 µM 1.60E-8 1 1.20E-13
19 SGTC_3094 9117742 7.23 49.5 µM 2.43E-8 1 2.46E-13
20 SGTC_1750 st081859 7.22 2.4 µM 2.48E-8 endomembrane recycling 84 2.54E-13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.403 5.34E-131 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.390 5.81E-122 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.387 4.52E-120 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.331 2.68E-86 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.311 2.67E-76 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.305 5.97E-73 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.292 5.26E-67 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.282 1.96E-62 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.267 7.40E-56 YER083C GET2 hom Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
0.264 2.02E-54 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.244 1.41E-46 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.241 1.19E-45 YLR065C ENV10 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
0.239 1.18E-44 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.238 2.54E-44 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.235 4.09E-43 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore