HIPHOP chemogenomics database

YDL101C / DUN1

Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair

Zygosity: Homozygous strain

fixedexpanded
Profile for YDL101C / DUN1

Click on Significant Values for Screen Details

Top fitness defect scores for YDL101C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2003	4023581	12.10	200.0 µM	2.45E-30	cell wall signaling	2	4.67E-34
2	SGTC_2004	4023728	8.69	41.9 µM	1.47E-16		2	1.74E-18
3	SGTC_2619	obliquin	7.02	87.9 µM	2.01E-11	cell wall	58	1.10E-12
4	SGTC_273	hydroxyurea	6.02	18.1 mM	7.58E-9	heme biosynthesis & mitochondrial translocase	10	8.87E-10
5	SGTC_3247	9135149	5.75	49.5 µM	3.24E-8	DNA damage response	13	4.57E-9
6	SGTC_1915	st067105	5.51	30.6 µM	1.12E-7	RNA pol III & RNase P/MRP	9	1.84E-8
7	SGTC_2584	androsterone	5.18	48.7 µM	5.59E-7	cell wall	76	1.13E-7
8	SGTC_70	0141-0289	4.79	43.3 µM	3.25E-6	mitochondrial stress	120	8.20E-7
9	SGTC_1153	4055-0057	4.68	267.0 µM	5.26E-6		8	1.41E-6
10	SGTC_268	camptothecin	4.61	39.6 µM	7.07E-6	DNA damage response	54	1.97E-6
11	SGTC_599	1935-0052	4.54	86.5 µM	9.79E-6		9	2.84E-6
12	SGTC_2996	9066192	4.54	71.4 µM	9.83E-6		19	2.85E-6
13	SGTC_2429	5902844	4.50	64.4 µM	1.17E-5		10	3.47E-6
14	SGTC_1879	gemcitabine	4.47	100.0 µM	1.33E-5		6	4.00E-6
15	SGTC_3088	9117578	4.43	49.5 µM	1.55E-5	amide catabolism	6	4.74E-6
16	SGTC_788	1182-0090	4.42	188.0 µM	1.62E-5	endomembrane recycling	67	5.02E-6
17	SGTC_744	1594-0068	4.39	283.0 µM	1.80E-5	amide catabolism	18	5.62E-6
18	SGTC_2588	indole-3-carbinol	4.38	100.0 µM	1.89E-5		30	5.94E-6
19	SGTC_1467	k284-1082	4.27	293.0 µM	2.92E-5		10	9.69E-6
20	SGTC_2008	4012253	4.20	172.0 µM	3.83E-5		19	1.32E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.172	1.19E-23	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.157	6.06E-20	YBR202W	MCM7	het	Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
0.145	2.76E-17	YGL033W	HOP2	hom	Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.139	6.36E-16	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.139	6.54E-16	YLR342W	FKS1	hom	Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.136	2.76E-15	YJL027C_p	YJL027C_p	hom	Putative protein of unknown function
0.133	1.10E-14	YBL085W	BOI1	hom	Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.124	5.03E-13	YDR076W	RAD55	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.122	1.27E-12	YDR527W	RBA50	het	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.120	3.31E-12	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.120	3.63E-12	YGL175C	SAE2	hom	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.119	5.51E-12	YNL075W	IMP4	het	Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
0.118	6.55E-12	YPR143W	RRP15	het	Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.118	8.53E-12	YNL112W	DBP2	het	ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.116	1.37E-11	YNL273W	TOF1	hom	Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase