YDL117W / CYK3

SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL117W / CYK3

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:4.75|P-value:1.37E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.04|P-value:3.34E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.04|P-value:3.35E-7 ID:SGTC_139|Compound:0099-0334|FD-Score:3.25|P-value:6.51E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.88|P-value:7.15E-7 ID:SGTC_235|Compound:0330-0087|FD-Score:4.52|P-value:4.07E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.50|P-value:2.67E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.64|P-value:2.30E-6 ID:SGTC_360|Compound:0957-0399|FD-Score:3.54|P-value:2.33E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.64|P-value:1.59E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.31|P-value:5.22E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:4.78|P-value:1.19E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.48|P-value:2.94E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-3.18|P-value:9.70E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-3.41|P-value:4.51E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.46|P-value:5.21E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:3.67|P-value:1.44E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:9.22|P-value:5.54E-20 ID:SGTC_974|Compound:1326-0144|FD-Score:4.32|P-value:1.00E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:7.36|P-value:2.15E-13 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-4.46|P-value:6.83E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.69|P-value:1.35E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.15|P-value:9.22E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.91|P-value:6.91E-5 ID:SGTC_1613|Compound:st002381|FD-Score:4.87|P-value:7.73E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.75|P-value:1.37E-6 ID:SGTC_1682|Compound:st012968|FD-Score:-4.83|P-value:1.21E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.85|P-value:7.07E-5 ID:SGTC_1931|Compound:st074438|FD-Score:3.80|P-value:8.77E-5 ID:SGTC_1944|Compound:st076371|FD-Score:3.46|P-value:3.14E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.80|P-value:8.55E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.59|P-value:1.95E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.19|P-value:7.94E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.15|P-value:2.08E-5 ID:SGTC_2210|Compound:7115885|FD-Score:3.89|P-value:5.97E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.79|P-value:9.00E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.54|P-value:2.80E-4 ID:SGTC_2286|Compound:7945697|FD-Score:5.18|P-value:1.61E-7 ID:SGTC_2289|Compound:7946404|FD-Score:17.40|P-value:1.05E-65 ID:SGTC_2311|Compound:7694451|FD-Score:3.21|P-value:7.45E-4 ID:SGTC_2356|Compound:9061756|FD-Score:-3.25|P-value:7.83E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.10|P-value:2.55E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.45|P-value:3.28E-4 ID:SGTC_2396|Compound:5342950|FD-Score:5.59|P-value:1.75E-8 ID:SGTC_2467|Compound:5474540|FD-Score:3.53|P-value:2.42E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-5.27|P-value:1.34E-7 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.72|P-value:1.42E-4 ID:SGTC_2702|Compound:st077768|FD-Score:3.36|P-value:4.42E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.65|P-value:1.54E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.40|P-value:3.81E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.39|P-value:4.00E-4 ID:SGTC_3002|Compound:9075603|FD-Score:4.09|P-value:2.66E-5 ID:SGTC_3016|Compound:9080866|FD-Score:4.10|P-value:2.52E-5 ID:SGTC_3311|Compound:9131146|FD-Score:3.91|P-value:5.58E-5 ID:SGTC_3322|Compound:9139058|FD-Score:5.60|P-value:1.70E-8 ID:SGTC_3334|Compound:9142812|FD-Score:3.55|P-value:2.26E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:4.75|P-value:1.37E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.04|P-value:3.34E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.04|P-value:3.35E-7 ID:SGTC_139|Compound:0099-0334|FD-Score:3.25|P-value:6.51E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.88|P-value:7.15E-7 ID:SGTC_235|Compound:0330-0087|FD-Score:4.52|P-value:4.07E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.50|P-value:2.67E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.64|P-value:2.30E-6 ID:SGTC_360|Compound:0957-0399|FD-Score:3.54|P-value:2.33E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.64|P-value:1.59E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.31|P-value:5.22E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:4.78|P-value:1.19E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.48|P-value:2.94E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-3.18|P-value:9.70E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-3.41|P-value:4.51E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.46|P-value:5.21E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:3.67|P-value:1.44E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:9.22|P-value:5.54E-20 ID:SGTC_974|Compound:1326-0144|FD-Score:4.32|P-value:1.00E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:7.36|P-value:2.15E-13 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-4.46|P-value:6.83E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.69|P-value:1.35E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.15|P-value:9.22E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.91|P-value:6.91E-5 ID:SGTC_1613|Compound:st002381|FD-Score:4.87|P-value:7.73E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.75|P-value:1.37E-6 ID:SGTC_1682|Compound:st012968|FD-Score:-4.83|P-value:1.21E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.85|P-value:7.07E-5 ID:SGTC_1931|Compound:st074438|FD-Score:3.80|P-value:8.77E-5 ID:SGTC_1944|Compound:st076371|FD-Score:3.46|P-value:3.14E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.80|P-value:8.55E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.59|P-value:1.95E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.19|P-value:7.94E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.15|P-value:2.08E-5 ID:SGTC_2210|Compound:7115885|FD-Score:3.89|P-value:5.97E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.79|P-value:9.00E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.54|P-value:2.80E-4 ID:SGTC_2286|Compound:7945697|FD-Score:5.18|P-value:1.61E-7 ID:SGTC_2289|Compound:7946404|FD-Score:17.40|P-value:1.05E-65 ID:SGTC_2311|Compound:7694451|FD-Score:3.21|P-value:7.45E-4 ID:SGTC_2356|Compound:9061756|FD-Score:-3.25|P-value:7.83E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.10|P-value:2.55E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.45|P-value:3.28E-4 ID:SGTC_2396|Compound:5342950|FD-Score:5.59|P-value:1.75E-8 ID:SGTC_2467|Compound:5474540|FD-Score:3.53|P-value:2.42E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-5.27|P-value:1.34E-7 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.72|P-value:1.42E-4 ID:SGTC_2702|Compound:st077768|FD-Score:3.36|P-value:4.42E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.65|P-value:1.54E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.40|P-value:3.81E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.39|P-value:4.00E-4 ID:SGTC_3002|Compound:9075603|FD-Score:4.09|P-value:2.66E-5 ID:SGTC_3016|Compound:9080866|FD-Score:4.10|P-value:2.52E-5 ID:SGTC_3311|Compound:9131146|FD-Score:3.91|P-value:5.58E-5 ID:SGTC_3322|Compound:9139058|FD-Score:5.60|P-value:1.70E-8 ID:SGTC_3334|Compound:9142812|FD-Score:3.55|P-value:2.26E-4

Top fitness defect scores for YDL117W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2289 7946404 17.40 165.1 µM 1.05E-65 1 6.61E-68
2 SGTC_839 0122-0028 9.22 230.0 µM 5.54E-20 RSC complex & mRNA processing 1 1.46E-20
3 SGTC_1133 3473-0247 7.36 50.0 µM 2.15E-13 RSC complex & mRNA processing 6 9.51E-14
4 SGTC_3322 9139058 5.60 2.1 µM 1.70E-8 cell wall 7 1.09E-8
5 SGTC_2396 5342950 5.59 42.8 µM 1.75E-8 RSC complex & mRNA processing 16 1.12E-8
6 SGTC_2286 7945697 5.18 125.0 µM 1.61E-7 27 1.11E-7
7 SGTC_122 0236-0016 5.04 402.4 µM 3.35E-7 RNA processing & uracil transport 14 2.37E-7
8 SGTC_221 trifluoperazine 4.88 10.3 µM 7.15E-7 NEO1-PIK1 166 5.18E-7
9 SGTC_1613 st002381 4.87 52.1 µM 7.73E-7 10 5.61E-7
10 SGTC_594 1487-1584 4.78 10.8 µM 1.19E-6 13 8.71E-7
11 SGTC_1679 4130-1276 4.75 6.7 µM 1.37E-6 Golgi 43 1.01E-6
12 SGTC_65 0798-0905 4.75 35.0 µM 1.37E-6 calcium & mitochondrial duress 59 1.01E-6
13 SGTC_351 caspofungin 4.64 25.0 nM 2.30E-6 cell wall 67 1.73E-6
14 SGTC_235 0330-0087 4.52 5.0 mM 4.07E-6 TSC3-RPN4 12 3.11E-6
15 SGTC_800 3448-7362 4.46 155.0 µM 5.21E-6 translation 123 4.01E-6
16 SGTC_974 1326-0144 4.32 138.0 µM 1.00E-5 ERG2 30 7.88E-6
17 SGTC_2150 5556613 4.15 200.0 µM 2.08E-5 17 1.67E-5
18 SGTC_3016 9080866 4.10 49.5 µM 2.52E-5 23 2.04E-5
19 SGTC_2365 9042475 4.10 200.0 µM 2.55E-5 32 2.06E-5
20 SGTC_3002 9075603 4.09 71.4 µM 2.66E-5 22 2.15E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.226 3.31E-40 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.225 7.46E-40 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.218 2.82E-37 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.204 8.87E-33 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.195 3.62E-30 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.181 3.17E-26 YJL139C YUR1 hom Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
0.180 9.88E-26 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.168 9.90E-23 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.168 1.38E-22 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.159 1.77E-20 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.159 2.21E-20 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.158 4.11E-20 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.157 4.78E-20 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.153 4.67E-19 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.152 6.60E-19 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication