YDL118W_d

Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL118W / YDL118W

Click on Significant Values for Screen Details

Top fitness defect scores for YDL118W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1788 fluconazole 28.30 20.0 µM 3.62E-133 azole & statin 2 8.17E-177
2 SGTC_227 fluconazole 16.20 33.5 µM 3.70E-45 azole & statin 4 1.46E-59
3 SGTC_171 k064-0041 14.30 9.7 µM 1.87E-35 heme requiring 2 1.22E-46
4 SGTC_350 k064-0020 13.40 19.8 µM 3.13E-31 heme requiring 2 5.13E-41
5 SGTC_1712 st032288 11.10 53.4 µM 4.40E-22 calcium & mitochondrial duress 18 7.67E-29
6 SGTC_1226 3909-7979 10.40 35.4 µM 9.76E-20 5 1.01E-25
7 SGTC_902 1120-0002 9.61 2.1 µM 4.55E-17 azole & statin 4 3.58E-22
8 SGTC_1991 st073000 9.43 8.9 µM 1.69E-16 NEO1 12 2.04E-21
9 SGTC_2347 9049783 9.25 174.8 µM 5.99E-16 3 1.10E-20
10 SGTC_2647 atorvastatin 8.97 60.2 µM 4.19E-15 azole & statin 3 1.46E-19
11 SGTC_1311 1190-0148 8.46 100.0 µM 1.29E-13 azole & statin 2 1.39E-17
12 SGTC_2648 atorvastatin 8.36 78.7 µM 2.42E-13 azole & statin 4 3.20E-17
13 SGTC_2651 1327-0036 8.06 5.0 µM 1.59E-12 37 3.90E-16
14 SGTC_1495 4469-0029 7.99 237.0 µM 2.44E-12 24 6.92E-16
15 SGTC_712 r015-0009 7.71 80.6 µM 1.32E-11 3 6.47E-15
16 SGTC_831 k061-1017 7.51 217.0 µM 4.08E-11 translation 2 2.90E-14
17 SGTC_73 0327-0325 7.37 16.2 µM 9.15E-11 DNA damage response 8 8.48E-14
18 SGTC_2930 9013230 7.26 14.9 µM 1.72E-10 heme biosynthesis & mitochondrial translocase 3 1.96E-13
19 SGTC_547 1120-0019 7.03 610.0 nM 5.95E-10 azole & statin 5 1.02E-12
20 SGTC_1774 st048371 7.00 48.5 µM 7.01E-10 RNA processing & uracil transport 4 1.26E-12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.599 0 YDL119C_p YDL119C_p hom Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.373 3.31E-111 YHR045W_p YHR045W_p hom Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
0.369 8.92E-109 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.316 1.39E-78 YPL170W DAP1 hom Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
0.302 1.26E-71 YBR035C PDX3 hom Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
0.280 1.82E-61 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.280 2.40E-61 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.259 1.89E-52 YMR063W RIM9 hom Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
0.253 2.84E-50 YDR232W HEM1 het 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.227 1.41E-40 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.210 1.09E-34 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.201 5.10E-32 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.198 6.60E-31 YJR049C UTR1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.193 2.26E-29 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.189 1.92E-28 YHL027W RIM101 hom Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC