YDL131W / LYS21

Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL131W / LYS21

Click on Significant Values for Screen Details ID:SGTC_264|Compound:promethazine|FD-Score:-5.46|P-value:4.22E-10 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.23|P-value:8.33E-7 ID:SGTC_456|Compound:gingerol|FD-Score:-3.86|P-value:7.43E-6 ID:SGTC_708|Compound:3448-5400|FD-Score:-2.87|P-value:6.30E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:-3.51|P-value:4.12E-5 ID:SGTC_729|Compound:4092-0585|FD-Score:-2.98|P-value:4.04E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-2.97|P-value:4.32E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.94|P-value:4.40E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.30|P-value:9.24E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.16|P-value:1.45E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.15|P-value:2.03E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-2.76|P-value:9.83E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-4.19|P-value:1.22E-6 ID:SGTC_1740|Compound:st038286|FD-Score:3.07|P-value:2.50E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.44|P-value:4.84E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:2.85|P-value:6.25E-4 ID:SGTC_2255|Compound:7941087|FD-Score:-2.98|P-value:4.17E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-2.79|P-value:8.65E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-4.17|P-value:1.36E-6 ID:SGTC_2401|Compound:5128683|FD-Score:-4.44|P-value:3.09E-7 ID:SGTC_2423|Compound:st077119|FD-Score:-2.87|P-value:6.50E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.00|P-value:3.37E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.46|P-value:4.39E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.08|P-value:2.72E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.91|P-value:4.95E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.28|P-value:1.01E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.29|P-value:1.09E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.48|P-value:4.15E-5 ID:SGTC_2989|Compound:9073649|FD-Score:6.62|P-value:3.68E-14 ID:SGTC_2997|Compound:9067075|FD-Score:7.44|P-value:2.14E-17 ID:SGTC_3001|Compound:9076041|FD-Score:-3.08|P-value:2.75E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-2.98|P-value:4.18E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.92|P-value:4.64E-6 ID:SGTC_3130|Compound:9127414|FD-Score:-3.18|P-value:1.77E-4 ID:SGTC_3156|Compound:9099931|FD-Score:2.82|P-value:6.92E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.85|P-value:6.76E-6 ID:SGTC_3314|Compound:9135860|FD-Score:3.09|P-value:2.32E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.85|P-value:6.23E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-2.95|P-value:4.60E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.19|P-value:1.72E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-2.87|P-value:6.35E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-5.46|P-value:4.22E-10 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.23|P-value:8.33E-7 ID:SGTC_456|Compound:gingerol|FD-Score:-3.86|P-value:7.43E-6 ID:SGTC_708|Compound:3448-5400|FD-Score:-2.87|P-value:6.30E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:-3.51|P-value:4.12E-5 ID:SGTC_729|Compound:4092-0585|FD-Score:-2.98|P-value:4.04E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-2.97|P-value:4.32E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.94|P-value:4.40E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.30|P-value:9.24E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.16|P-value:1.45E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.15|P-value:2.03E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-2.76|P-value:9.83E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-4.19|P-value:1.22E-6 ID:SGTC_1740|Compound:st038286|FD-Score:3.07|P-value:2.50E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.44|P-value:4.84E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:2.85|P-value:6.25E-4 ID:SGTC_2255|Compound:7941087|FD-Score:-2.98|P-value:4.17E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-2.79|P-value:8.65E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-4.17|P-value:1.36E-6 ID:SGTC_2401|Compound:5128683|FD-Score:-4.44|P-value:3.09E-7 ID:SGTC_2423|Compound:st077119|FD-Score:-2.87|P-value:6.50E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.00|P-value:3.37E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.46|P-value:4.39E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.08|P-value:2.72E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.91|P-value:4.95E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.28|P-value:1.01E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.29|P-value:1.09E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.48|P-value:4.15E-5 ID:SGTC_2989|Compound:9073649|FD-Score:6.62|P-value:3.68E-14 ID:SGTC_2997|Compound:9067075|FD-Score:7.44|P-value:2.14E-17 ID:SGTC_3001|Compound:9076041|FD-Score:-3.08|P-value:2.75E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-2.98|P-value:4.18E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.92|P-value:4.64E-6 ID:SGTC_3130|Compound:9127414|FD-Score:-3.18|P-value:1.77E-4 ID:SGTC_3156|Compound:9099931|FD-Score:2.82|P-value:6.92E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.85|P-value:6.76E-6 ID:SGTC_3314|Compound:9135860|FD-Score:3.09|P-value:2.32E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.85|P-value:6.23E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-2.95|P-value:4.60E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.19|P-value:1.72E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-2.87|P-value:6.35E-4

Top fitness defect scores for YDL131W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2997 9067075 7.44 71.4 µM 2.14E-17 unfolded protein response 8 5.01E-14
2 SGTC_2989 9073649 6.62 71.4 µM 3.68E-14 7 1.78E-11
3 SGTC_418 lividomycin a 4.23 100.0 µM 8.33E-7 8 1.14E-5
4 SGTC_3125 9127534 3.92 49.5 µM 4.64E-6 12 4.46E-5
5 SGTC_2871 9038510 3.91 13.0 µM 4.95E-6 17 4.70E-5
6 SGTC_3313 9133968 3.85 57.0 µM 6.76E-6 8 6.01E-5
7 SGTC_2947 9072639 3.48 7.7 µM 4.15E-5 tubulin folding & SWR complex 62 2.54E-4
8 SGTC_2605 digitonin 3.46 300.0 nM 4.39E-5 47 2.65E-4
9 SGTC_1924 st058459 3.44 75.1 µM 4.84E-5 21 2.87E-4
10 SGTC_1263 0774-1208 3.30 64.8 µM 9.24E-5 79 4.80E-4
11 SGTC_2876 9059903 3.28 26.0 µM 1.01E-4 33 5.16E-4
12 SGTC_3314 9135860 3.09 59.4 µM 2.32E-4 52 9.97E-4
13 SGTC_1740 st038286 3.07 13.2 µM 2.50E-4 69 0.00106
14 SGTC_2429 5902844 3.00 64.4 µM 3.37E-4 83 0.00134
15 SGTC_1253 0550-0034 2.94 176.0 µM 4.40E-4 67 0.00166
16 SGTC_3315 9136395 2.85 68.5 µM 6.23E-4 54 0.00219
17 SGTC_1928 4'-methoxychalcone 2.85 40.3 µM 6.25E-4 48 0.00220
18 SGTC_3156 9099931 2.82 49.5 µM 6.92E-4 55 0.00238
19 SGTC_459 nigericin 2.71 15.3 µM 0.00108 137 0.00340
20 SGTC_1911 st060208 2.68 74.3 µM 0.00118 102 0.00364

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.263 5.89E-54 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.253 2.68E-50 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.222 1.17E-38 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.217 6.74E-37 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.209 1.79E-34 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.197 1.28E-30 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.183 1.12E-26 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.178 2.06E-25 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.177 4.16E-25 YDR459C PFA5 hom Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
0.177 5.67E-25 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.176 8.95E-25 YJL131C AIM23 hom Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
0.175 1.65E-24 YDL227C HO hom Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
0.168 1.39E-22 YDL199C_p YDL199C_p hom Putative transporter, member of the sugar porter family
0.166 2.98E-22 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.163 2.20E-21 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability