YDL132W / CDC53

Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL132W / CDC53

Click on Significant Values for Screen Details ID:SGTC_149|Compound:3970-0822|FD-Score:4.24|P-value:5.87E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.71|P-value:3.75E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.69|P-value:4.06E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.83|P-value:2.55E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.61|P-value:5.18E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:4.32|P-value:4.51E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.70|P-value:3.92E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:5.05|P-value:2.31E-6 ID:SGTC_981|Compound:1488-1094|FD-Score:3.99|P-value:1.47E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.80|P-value:6.73E-6 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.61|P-value:5.66E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:-3.45|P-value:9.42E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.51|P-value:7.11E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.90|P-value:2.24E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:-4.30|P-value:5.30E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.63|P-value:4.90E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.67|P-value:4.35E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-4.81|P-value:7.26E-6 ID:SGTC_1611|Compound:st001856|FD-Score:-4.64|P-value:1.40E-5 ID:SGTC_1729|Compound:st035615|FD-Score:4.25|P-value:5.70E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-3.68|P-value:4.61E-4 ID:SGTC_1898|Compound:st060199|FD-Score:4.72|P-value:9.44E-6 ID:SGTC_1958|Compound:st076513|FD-Score:3.50|P-value:7.36E-4 ID:SGTC_2136|Compound:5328288|FD-Score:3.54|P-value:6.47E-4 ID:SGTC_2274|Compound:7947094|FD-Score:3.60|P-value:5.47E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.55|P-value:2.04E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.41|P-value:9.90E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.11|P-value:1.81E-6 ID:SGTC_2424|Compound:5537928|FD-Score:3.63|P-value:4.84E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.33|P-value:4.20E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.63|P-value:4.92E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-3.54|P-value:7.15E-4 ID:SGTC_3012|Compound:9080433|FD-Score:-4.66|P-value:1.33E-5 ID:SGTC_3210|Compound:9128157|FD-Score:4.83|P-value:5.78E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:4.24|P-value:5.87E-5 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.71|P-value:3.75E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.69|P-value:4.06E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.83|P-value:2.55E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.61|P-value:5.18E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:4.32|P-value:4.51E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.70|P-value:3.92E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:5.05|P-value:2.31E-6 ID:SGTC_981|Compound:1488-1094|FD-Score:3.99|P-value:1.47E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.80|P-value:6.73E-6 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.61|P-value:5.66E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:-3.45|P-value:9.42E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:3.51|P-value:7.11E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.90|P-value:2.24E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:-4.30|P-value:5.30E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.63|P-value:4.90E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.67|P-value:4.35E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-4.81|P-value:7.26E-6 ID:SGTC_1611|Compound:st001856|FD-Score:-4.64|P-value:1.40E-5 ID:SGTC_1729|Compound:st035615|FD-Score:4.25|P-value:5.70E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-3.68|P-value:4.61E-4 ID:SGTC_1898|Compound:st060199|FD-Score:4.72|P-value:9.44E-6 ID:SGTC_1958|Compound:st076513|FD-Score:3.50|P-value:7.36E-4 ID:SGTC_2136|Compound:5328288|FD-Score:3.54|P-value:6.47E-4 ID:SGTC_2274|Compound:7947094|FD-Score:3.60|P-value:5.47E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.55|P-value:2.04E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.41|P-value:9.90E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.11|P-value:1.81E-6 ID:SGTC_2424|Compound:5537928|FD-Score:3.63|P-value:4.84E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.33|P-value:4.20E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.63|P-value:4.92E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-3.54|P-value:7.15E-4 ID:SGTC_3012|Compound:9080433|FD-Score:-4.66|P-value:1.33E-5 ID:SGTC_3210|Compound:9128157|FD-Score:4.83|P-value:5.78E-6

Top fitness defect scores for YDL132W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2419 paclitaxel 5.11 166.5 µM 1.81E-6 2 1.64E-7 0.57
2 SGTC_977 1348-1378 5.05 135.0 µM 2.31E-6 3 2.20E-7 0.52
3 SGTC_3210 9128157 4.83 49.5 µM 5.78E-6 4 6.66E-7 0.07
4 SGTC_1064 honokiol 4.80 6.8 µM 6.73E-6 4 8.01E-7 0.23
5 SGTC_1898 st060199 4.72 18.6 µM 9.44E-6 2 1.21E-6 0.55
6 SGTC_2434 5670830 4.33 169.9 µM 4.20E-5 2 7.32E-6 0.13
7 SGTC_799 1310-0557 4.32 151.0 µM 4.51E-5 superoxide 3 7.96E-6 0.14
8 SGTC_1729 st035615 4.25 46.0 µM 5.70E-5 6 1.06E-5 0.01
9 SGTC_149 3970-0822 4.24 46.1 µM 5.87E-5 tubulin folding & SWR complex 8 1.10E-5 0.15
10 SGTC_981 1488-1094 3.99 54.9 µM 1.47E-4 2 3.33E-5 0.20
11 SGTC_598 3937-0236 3.83 20.2 µM 2.55E-4 ERG2 4 6.45E-5 0.11
12 SGTC_412 gemfibrozil 3.71 100.0 µM 3.75E-4 DNA intercalators 8 1.02E-4 0.14
13 SGTC_806 3966-4455 3.70 5.9 µM 3.92E-4 9 1.08E-4 0.04
14 SGTC_512 prima-1 3.69 270.0 µM 4.06E-4 DNA intercalators 16 1.13E-4 0.09
15 SGTC_1565 sinapyl alcohol 3.67 95.1 µM 4.35E-4 5 1.23E-4 0.03
16 SGTC_2424 5537928 3.63 59.2 µM 4.84E-4 12 1.39E-4 0.01
17 SGTC_1484 3473-2379 3.63 286.0 µM 4.90E-4 3 1.41E-4 0.39
18 SGTC_2953 9074162 3.63 66.5 µM 4.92E-4 8 1.42E-4 0.21
19 SGTC_783 0848-0141 3.61 92.7 µM 5.18E-4 RSC & ERG11 18 1.51E-4 0.03
20 SGTC_2274 7947094 3.60 46.1 µM 5.47E-4 9 1.61E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 3.97E-11 YLL006W-A_p YLL006W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.100 5.96E-9 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.098 1.15E-8 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.091 1.19E-7 YIL084C SDS3 hom Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
0.091 1.20E-7 YLR029C RPL15A het Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
0.087 3.88E-7 YDR464W SPP41 het Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
0.087 4.12E-7 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.081 2.77E-6 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.077 7.41E-6 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.075 1.54E-5 YPR044C_d OPI11_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO
0.074 1.91E-5 YHR080C YHR080C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
0.071 3.49E-5 YPR148C YPR148C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.070 5.18E-5 YLR406C RPL31B hom Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
0.069 6.84E-5 YPR038W_d IRC16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci
0.068 8.11E-5 YDR409W SIZ1 hom SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring