YDL137W / ARF2

ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL137W / ARF2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.83|P-value:9.22E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:4.16|P-value:2.40E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.54|P-value:4.64E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.56|P-value:4.13E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.54|P-value:2.68E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.29|P-value:6.57E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.88|P-value:7.63E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.33|P-value:5.68E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:6.76|P-value:2.11E-11 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.41|P-value:4.31E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:3.38|P-value:4.93E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:3.45|P-value:3.76E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-4.22|P-value:1.84E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.11|P-value:2.94E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.23|P-value:8.16E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.36|P-value:1.02E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:3.41|P-value:4.32E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.51|P-value:3.03E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.21|P-value:6.57E-10 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.58|P-value:2.41E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.47|P-value:3.48E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.29|P-value:1.19E-7 ID:SGTC_1764|Compound:st044502|FD-Score:-3.28|P-value:6.74E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.33|P-value:5.60E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.20|P-value:2.05E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.68|P-value:1.63E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.73|P-value:1.37E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.94|P-value:7.06E-7 ID:SGTC_2056|Compound:5235370|FD-Score:-3.76|P-value:1.20E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.38|P-value:4.84E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.58|P-value:2.38E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.46|P-value:3.54E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-5.39|P-value:6.85E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.31|P-value:1.24E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.61|P-value:3.37E-6 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.37|P-value:5.01E-4 ID:SGTC_2786|Compound:7743617|FD-Score:5.15|P-value:2.48E-7 ID:SGTC_2906|Compound:7926292|FD-Score:3.67|P-value:1.71E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.46|P-value:3.69E-4 ID:SGTC_2939|Compound:9044578|FD-Score:3.92|P-value:6.38E-5 ID:SGTC_2953|Compound:9074162|FD-Score:-3.37|P-value:4.97E-4 ID:SGTC_3011|Compound:9081274|FD-Score:-3.18|P-value:9.47E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-3.71|P-value:1.44E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.37|P-value:5.09E-4 ID:SGTC_3121|Compound:9125696|FD-Score:3.30|P-value:6.31E-4 ID:SGTC_3151|Compound:9099821|FD-Score:5.34|P-value:9.33E-8 ID:SGTC_3154|Compound:9100113|FD-Score:3.91|P-value:6.84E-5 ID:SGTC_3156|Compound:9099931|FD-Score:4.15|P-value:2.59E-5 ID:SGTC_3184|Compound:9108758|FD-Score:3.51|P-value:3.05E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-4.43|P-value:7.30E-6 ID:SGTC_3239|Compound:9133757|FD-Score:4.07|P-value:3.59E-5 ID:SGTC_3240|Compound:9133527|FD-Score:-4.16|P-value:2.39E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.80|P-value:1.05E-4 ID:SGTC_3268|Compound:9137683|FD-Score:3.51|P-value:3.09E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.83|P-value:9.22E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:4.16|P-value:2.40E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.54|P-value:4.64E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.56|P-value:4.13E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.54|P-value:2.68E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.29|P-value:6.57E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.88|P-value:7.63E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.33|P-value:5.68E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:6.76|P-value:2.11E-11 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.41|P-value:4.31E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:3.38|P-value:4.93E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:3.45|P-value:3.76E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-4.22|P-value:1.84E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.11|P-value:2.94E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.23|P-value:8.16E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.36|P-value:1.02E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:3.41|P-value:4.32E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.51|P-value:3.03E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.21|P-value:6.57E-10 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.58|P-value:2.41E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.47|P-value:3.48E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.29|P-value:1.19E-7 ID:SGTC_1764|Compound:st044502|FD-Score:-3.28|P-value:6.74E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.33|P-value:5.60E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.20|P-value:2.05E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.68|P-value:1.63E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.73|P-value:1.37E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.94|P-value:7.06E-7 ID:SGTC_2056|Compound:5235370|FD-Score:-3.76|P-value:1.20E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.38|P-value:4.84E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.58|P-value:2.38E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.46|P-value:3.54E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-5.39|P-value:6.85E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.31|P-value:1.24E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.61|P-value:3.37E-6 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.37|P-value:5.01E-4 ID:SGTC_2786|Compound:7743617|FD-Score:5.15|P-value:2.48E-7 ID:SGTC_2906|Compound:7926292|FD-Score:3.67|P-value:1.71E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.46|P-value:3.69E-4 ID:SGTC_2939|Compound:9044578|FD-Score:3.92|P-value:6.38E-5 ID:SGTC_2953|Compound:9074162|FD-Score:-3.37|P-value:4.97E-4 ID:SGTC_3011|Compound:9081274|FD-Score:-3.18|P-value:9.47E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-3.71|P-value:1.44E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.37|P-value:5.09E-4 ID:SGTC_3121|Compound:9125696|FD-Score:3.30|P-value:6.31E-4 ID:SGTC_3151|Compound:9099821|FD-Score:5.34|P-value:9.33E-8 ID:SGTC_3154|Compound:9100113|FD-Score:3.91|P-value:6.84E-5 ID:SGTC_3156|Compound:9099931|FD-Score:4.15|P-value:2.59E-5 ID:SGTC_3184|Compound:9108758|FD-Score:3.51|P-value:3.05E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-4.43|P-value:7.30E-6 ID:SGTC_3239|Compound:9133757|FD-Score:4.07|P-value:3.59E-5 ID:SGTC_3240|Compound:9133527|FD-Score:-4.16|P-value:2.39E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.80|P-value:1.05E-4 ID:SGTC_3268|Compound:9137683|FD-Score:3.51|P-value:3.09E-4

Top fitness defect scores for YDL137W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_873 0591-4978 6.76 488.0 µM 2.11E-11 2 7.14E-12
2 SGTC_3151 9099821 5.34 49.5 µM 9.33E-8 5 4.69E-8
3 SGTC_2786 7743617 5.15 45.5 µM 2.48E-7 6 1.30E-7
4 SGTC_2021 4019055 4.94 173.0 µM 7.06E-7 endomembrane recycling 66 3.90E-7
5 SGTC_2688 kanamycin b 4.61 96.7 µM 3.37E-6 calcium & mitochondrial duress 283 2.00E-6
6 SGTC_77 0828-0286 4.54 12.5 µM 4.64E-6 TSC3-RPN4 20 2.80E-6
7 SGTC_1283 0922-0125 4.36 16.4 µM 1.02E-5 15 6.41E-6
8 SGTC_71 0417-1750 4.16 47.8 µM 2.40E-5 Golgi 39 1.56E-5
9 SGTC_3156 9099931 4.15 49.5 µM 2.59E-5 5 1.69E-5
10 SGTC_3239 9133757 4.07 49.5 µM 3.59E-5 2 2.38E-5
11 SGTC_2939 9044578 3.92 71.4 µM 6.38E-5 38 4.35E-5
12 SGTC_3154 9100113 3.91 49.5 µM 6.84E-5 29 4.67E-5
13 SGTC_528 3492-0019 3.88 172.0 µM 7.63E-5 calcium & mitochondrial duress 226 5.24E-5
14 SGTC_15 0266-0086 3.83 27.1 µM 9.22E-5 endomembrane recycling 68 6.38E-5
15 SGTC_3257 9139206 3.80 33.0 µM 1.05E-4 5 7.34E-5
16 SGTC_1951 sophoraflavanone G 3.73 9.2 µM 1.37E-4 RNA processing & uracil transport 31 9.68E-5
17 SGTC_1919 st067113 3.68 52.3 µM 1.63E-4 TSC3-RPN4 39 1.16E-4
18 SGTC_2906 7926292 3.67 26.4 µM 1.71E-4 35 1.22E-4
19 SGTC_2303 7774051 3.58 200.0 µM 2.38E-4 34 1.72E-4
20 SGTC_1597 7-benzyloxygramine 3.58 47.5 µM 2.41E-4 calcium & mitochondrial duress 151 1.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 9.10E-26 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.179 1.22E-25 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.177 5.03E-25 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.155 1.28E-19 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.155 2.11E-19 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.140 3.96E-16 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.138 9.20E-16 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.137 1.39E-15 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.131 2.58E-14 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.129 7.06E-14 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.127 1.40E-13 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.126 2.51E-13 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.123 7.27E-13 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.122 1.37E-12 YKR094C RPL40B hom Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
0.121 2.33E-12 YNR044W AGA1 hom Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds