YDL139C / SCM3

Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL139C / SCM3

Click on Significant Values for Screen Details ID:SGTC_139|Compound:0099-0334|FD-Score:3.60|P-value:6.02E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.32|P-value:1.67E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:2.97|P-value:7.80E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-4.12|P-value:4.40E-6 ID:SGTC_324|Compound:1315-0088|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-4.34|P-value:1.44E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:-3.28|P-value:1.92E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:-2.93|P-value:7.72E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.43|P-value:1.10E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.41|P-value:1.37E-4 ID:SGTC_442|Compound:cape|FD-Score:3.10|P-value:4.70E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.06|P-value:4.59E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.83|P-value:2.20E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:2.96|P-value:8.03E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:3.45|P-value:1.17E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.99|P-value:1.00E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:3.09|P-value:4.93E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:3.59|P-value:6.37E-5 ID:SGTC_903|Compound:1134-0042|FD-Score:5.15|P-value:1.85E-8 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.22|P-value:2.49E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-2.94|P-value:7.27E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.16|P-value:3.74E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.30|P-value:2.20E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.23|P-value:2.85E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.05|P-value:4.88E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.20|P-value:3.21E-4 ID:SGTC_1985|Compound:st071382|FD-Score:2.99|P-value:7.31E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:2.93|P-value:9.08E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.26|P-value:2.55E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.19|P-value:3.33E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-2.87|P-value:9.42E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.96|P-value:1.20E-5 ID:SGTC_2683|Compound:piperine|FD-Score:3.19|P-value:3.35E-4 ID:SGTC_2694|Compound:st077685|FD-Score:2.94|P-value:8.78E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.07|P-value:5.32E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-5.02|P-value:3.13E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-3.10|P-value:3.98E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-3.13|P-value:3.53E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-6.14|P-value:1.84E-11 ID:SGTC_3020|Compound:9089140|FD-Score:3.08|P-value:5.18E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.29|P-value:2.22E-4 ID:SGTC_3255|Compound:9139072|FD-Score:2.95|P-value:8.33E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.83|P-value:2.14E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:3.60|P-value:6.02E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.32|P-value:1.67E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:2.97|P-value:7.80E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-4.12|P-value:4.40E-6 ID:SGTC_324|Compound:1315-0088|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-4.34|P-value:1.44E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:-3.28|P-value:1.92E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:-2.93|P-value:7.72E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.43|P-value:1.10E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.41|P-value:1.37E-4 ID:SGTC_442|Compound:cape|FD-Score:3.10|P-value:4.70E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.06|P-value:4.59E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.83|P-value:2.20E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:2.96|P-value:8.03E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:3.45|P-value:1.17E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.99|P-value:1.00E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:3.09|P-value:4.93E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:3.59|P-value:6.37E-5 ID:SGTC_903|Compound:1134-0042|FD-Score:5.15|P-value:1.85E-8 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.22|P-value:2.49E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-2.94|P-value:7.27E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.16|P-value:3.74E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.30|P-value:2.20E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.23|P-value:2.85E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.05|P-value:4.88E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.20|P-value:3.21E-4 ID:SGTC_1985|Compound:st071382|FD-Score:2.99|P-value:7.31E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:2.93|P-value:9.08E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.26|P-value:2.55E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.19|P-value:3.33E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-2.87|P-value:9.42E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.96|P-value:1.20E-5 ID:SGTC_2683|Compound:piperine|FD-Score:3.19|P-value:3.35E-4 ID:SGTC_2694|Compound:st077685|FD-Score:2.94|P-value:8.78E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.07|P-value:5.32E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-5.02|P-value:3.13E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-3.10|P-value:3.98E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-3.13|P-value:3.53E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-6.14|P-value:1.84E-11 ID:SGTC_3020|Compound:9089140|FD-Score:3.08|P-value:5.18E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.29|P-value:2.22E-4 ID:SGTC_3255|Compound:9139072|FD-Score:2.95|P-value:8.33E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.83|P-value:2.14E-5

Top fitness defect scores for YDL139C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_903 1134-0042 5.15 114.0 µM 1.85E-8 4 1.31E-7 0.47
2 SGTC_381 3461-2219 4.43 42.7 µM 1.10E-6 copper-dependent oxidative stress 2 4.67E-6 0.86
3 SGTC_785 1270-0111 3.99 65.3 µM 1.00E-5 7 3.24E-5 0.03
4 SGTC_2395 5646831 3.96 180.2 µM 1.20E-5 2 3.78E-5 0.17
5 SGTC_324 1315-0088 3.94 19.6 µM 1.32E-5 RSC & ERG11 14 4.13E-5 0.35
6 SGTC_3272 9137413 3.83 49.5 µM 2.14E-5 7 6.31E-5 0.06
7 SGTC_760 0568-0725 3.83 82.9 µM 2.20E-5 1 6.45E-5 0.18
8 SGTC_139 0099-0334 3.60 718.6 µM 6.02E-5 9 1.56E-4 0.01
9 SGTC_881 0833-0569 3.59 170.0 µM 6.37E-5 6 1.64E-4 0.28
10 SGTC_772 k072-0172 3.45 35.3 µM 1.17E-4 5 2.81E-4 0.28
11 SGTC_384 k007-0144 3.41 5.2 µM 1.37E-4 ERAD & cell cycle 20 3.23E-4 0.02
12 SGTC_1486 3970-0793 3.30 4.5 µM 2.20E-4 6 4.90E-4 0.17
13 SGTC_3238 9133470 3.29 49.5 µM 2.22E-4 9 4.94E-4 0.07
14 SGTC_2292 7671323 3.26 168.1 µM 2.55E-4 RNA processing & uracil transport 20 5.58E-4 0.01
15 SGTC_1747 st037496 3.23 66.6 µM 2.85E-4 17 6.15E-4 0.11
16 SGTC_1975 st070967 3.20 30.8 µM 3.21E-4 22 6.82E-4 0.02
17 SGTC_2308 7584454 3.19 197.6 µM 3.33E-4 21 7.05E-4 0.02
18 SGTC_2683 piperine 3.19 63.0 µM 3.35E-4 5 7.09E-4 1.17E-4
19 SGTC_1198 1612-1005 3.16 86.7 µM 3.74E-4 10 7.81E-4 0.17
20 SGTC_442 cape 3.10 100.0 µM 4.70E-4 5 9.55E-4 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.46E-11 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.106 6.78E-10 YGR080W TWF1 hom Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly
0.103 2.76E-9 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.087 4.94E-7 YGR171C MSM1 hom Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
0.085 8.69E-7 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.079 4.21E-6 YNR060W FRE4 hom Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
0.077 8.90E-6 YFR020W_p YFR020W_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.075 1.22E-5 YGR046W TAM41 het Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
0.075 1.53E-5 YDR481C PHO8 hom Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
0.074 1.90E-5 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.073 2.21E-5 YHR177W_p YHR177W_p hom Putative protein of unknown function; overexpression causes a cell cycle delay or arrest
0.073 2.42E-5 YDL105W NSE4 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
0.068 7.71E-5 YOL030W GAS5 hom 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
0.067 1.09E-4 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.067 1.13E-4 YJL016W_p YJL016W_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species