Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YDL144C / YDL144C

Click on Significant Values for Screen Details

Top fitness defect scores for YDL144C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1761 st044296 5.24 37.0 µM 2.68E-7 4 8.06E-8
2 SGTC_2587 piperic acid 5.23 92.2 µM 2.81E-7 4 8.49E-8
3 SGTC_1163 k060-0048 5.01 49.9 µM 8.21E-7 9 2.74E-7
4 SGTC_1898 st060199 4.95 18.6 µM 1.08E-6 3 3.70E-7
5 SGTC_1077 5-fluorocytosine 4.93 377.0 nM 1.21E-6 exosome 5 4.18E-7
6 SGTC_2431 5490079 4.82 200.0 µM 1.99E-6 15 7.22E-7
7 SGTC_1206 3978-0017 4.70 132.0 µM 3.34E-6 ERG2 18 1.27E-6
8 SGTC_1523 st012844 4.61 44.7 µM 5.19E-6 60S ribosome export 6 2.06E-6
9 SGTC_1464 k208-0027 4.35 110.0 µM 1.56E-5 18 6.87E-6
10 SGTC_358 0075-0058 3.99 9.4 µM 6.62E-5 60 3.32E-5
11 SGTC_2021 4019055 3.94 173.0 µM 8.07E-5 endomembrane recycling 124 4.12E-5
12 SGTC_1888 azatryptophan 3.91 97.5 µM 8.91E-5 17 4.59E-5
13 SGTC_2481 5478948 3.87 45.2 µM 1.03E-4 PDR1 39 5.39E-5
14 SGTC_991 3996-0057 3.59 64.7 µM 2.89E-4 29 1.66E-4
15 SGTC_1329 1447-1697 3.54 50.6 µM 3.39E-4 26 1.97E-4
16 SGTC_265 mycophenolic acid 3.48 32.9 µM 4.17E-4 RPP1 & pyrimidine depletion 40 2.47E-4
17 SGTC_1773 st048542 3.46 73.6 µM 4.50E-4 70 2.68E-4
18 SGTC_3223 9129970 3.46 49.5 µM 4.59E-4 34 2.74E-4
19 SGTC_793 0488-0545 3.43 106.0 µM 5.01E-4 27 3.02E-4
20 SGTC_1030 k029-0042 3.36 36.7 µM 6.41E-4 TSC3-RPN4 53 3.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 2.00E-10 YKR094C RPL40B hom Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
0.106 7.19E-10 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.096 2.90E-8 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.094 5.31E-8 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.092 8.72E-8 YER076C_p YER076C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
0.091 1.47E-7 YOL082W ATG19 hom Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
0.090 1.80E-7 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.089 2.70E-7 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.084 1.03E-6 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.082 1.71E-6 YOR377W ATF1 hom Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
0.082 2.34E-6 YFL040W_p YFL040W_p hom Putative transporter, member of the sugar porter family; YFL040W is not an essential gene
0.082 2.23E-6 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.081 2.84E-6 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.080 3.34E-6 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.079 4.98E-6 YOL052C-A DDR2 hom Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication