HIPHOP chemogenomics database

YDL153C / SAS10

Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDL153C / SAS10

Click on Significant Values for Screen Details

Top fitness defect scores for YDL153C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2988	9073640	7.72	71.4 µM	9.49E-16		1	6.03E-15	1.43
2	SGTC_2602	kawain	6.06	100.0 µM	2.11E-10		1	6.69E-10	0.83
3	SGTC_1919	st067113	4.82	52.3 µM	3.35E-7	TSC3-RPN4	5	7.02E-7	0.35
4	SGTC_107	0358-0022	4.80	2.7 µM	3.76E-7	SWF1 & branched chain AA biosynthesis	4	7.83E-7	0.94
5	SGTC_119	0121-0043	4.40	24.8 µM	2.98E-6	mitochondrial stress	14	5.53E-6	0.10
6	SGTC_2595	hexamethylquercetagetin	4.37	100.0 µM	3.35E-6		3	6.18E-6	0.39
7	SGTC_3137	9097352	4.33	49.5 µM	4.07E-6		3	7.43E-6	0.07
8	SGTC_728	3030-5151	4.33	22.5 µM	4.18E-6		1	7.61E-6	0.44
9	SGTC_2594	a-mangostin	4.18	100.0 µM	8.29E-6		4	1.45E-5	0.01
10	SGTC_2376	9072922	4.06	81.3 µM	1.43E-5		3	2.44E-5	0.20
11	SGTC_2926	9010726	4.06	65.2 µM	1.45E-5		5	2.46E-5	0.08
12	SGTC_2068	5226020	4.04	147.0 µM	1.56E-5		6	2.64E-5	0.21
13	SGTC_1931	st074438	3.95	57.1 µM	2.34E-5		12	3.88E-5	0.09
14	SGTC_2765	myclobutanil	3.82	7.7 µM	4.19E-5	azole & statin	10	6.72E-5	0.16
15	SGTC_2900	5328528	3.80	85.3 µM	4.56E-5	Golgi	6	7.28E-5	0.10
16	SGTC_512	prima-1	3.78	270.0 µM	4.92E-5	DNA intercalators	15	7.83E-5	0.09
17	SGTC_3026	9090633	3.63	49.5 µM	9.17E-5		8	1.41E-4	0.05
18	SGTC_1594	piperine	3.59	70.1 µM	1.08E-4		4	1.64E-4	0.44
19	SGTC_3329	9141233	3.58	27.6 µM	1.13E-4		5	1.71E-4	0.16
20	SGTC_2759	disulfiram	3.53	3.4 µM	1.39E-4		28	2.09E-4	0.01

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.199	2.08E-31	YLR195C	NMT1	het	N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.193	2.10E-29	YBR155W	CNS1	het	TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.167	2.95E-22	YDR235W	PRP42	het	U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
0.163	2.78E-21	YER043C	SAH1	het	S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.159	1.88E-20	YKL152C	GPM1	het	Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
0.151	1.31E-18	YBL004W	UTP20	het	Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.139	6.47E-16	YNL163C	RIA1	het	Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
0.134	7.11E-15	YJL174W	KRE9	het	Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
0.134	7.50E-15	YDR023W	SES1	het	Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.127	1.44E-13	YBL084C	CDC27	het	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.119	4.03E-12	YKL033W	TTI1	het	Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
0.119	4.16E-12	YER171W	RAD3	het	5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.117	8.95E-12	YLL013C	PUF3	hom	Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
0.116	1.61E-11	YIR011C	STS1	het	Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
0.115	2.06E-11	YJL061W	NUP82	het	Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)