YDL154W / MSH5

Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL154W / MSH5

Click on Significant Values for Screen Details ID:SGTC_114|Compound:0364-0001|FD-Score:3.37|P-value:1.70E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.40|P-value:1.54E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.50|P-value:1.02E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.94|P-value:9.86E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.33|P-value:2.19E-6 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.04|P-value:7.02E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.68|P-value:4.78E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.26|P-value:2.69E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:-3.42|P-value:1.62E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.02|P-value:7.30E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.18|P-value:4.10E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.08|P-value:5.42E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.04|P-value:6.14E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:2.93|P-value:9.15E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.10|P-value:4.88E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:-3.25|P-value:3.15E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.77|P-value:3.10E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.15|P-value:4.12E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:3.96|P-value:1.35E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.62|P-value:4.68E-7 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.10|P-value:5.59E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.53|P-value:1.02E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.79|P-value:5.04E-10 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.36|P-value:2.01E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.37|P-value:1.74E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.89|P-value:1.82E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.09|P-value:5.22E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-2.94|P-value:9.96E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.29|P-value:2.41E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.00|P-value:7.86E-4 ID:SGTC_2243|Compound:6634756|FD-Score:3.33|P-value:1.99E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.59|P-value:7.73E-5 ID:SGTC_2351|Compound:9026858|FD-Score:5.60|P-value:1.43E-9 ID:SGTC_2396|Compound:5342950|FD-Score:3.09|P-value:5.15E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.63|P-value:5.75E-5 ID:SGTC_2447|Compound:5741742|FD-Score:3.47|P-value:1.14E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:9.07|P-value:3.92E-22 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-4.65|P-value:4.82E-7 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:6.51|P-value:2.61E-12 ID:SGTC_2556|Compound:citropten|FD-Score:3.00|P-value:7.19E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.45|P-value:1.16E-6 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.00|P-value:7.11E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.38|P-value:1.88E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.64|P-value:5.49E-5 ID:SGTC_2864|Compound:9034755|FD-Score:-3.52|P-value:1.06E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.61|P-value:6.26E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.06|P-value:5.69E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.40|P-value:1.54E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.27|P-value:2.59E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.34|P-value:1.94E-4 ID:SGTC_3260|Compound:9137109|FD-Score:3.35|P-value:1.84E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.37|P-value:1.70E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.40|P-value:1.54E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.50|P-value:1.02E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.94|P-value:9.86E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.33|P-value:2.19E-6 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.04|P-value:7.02E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.68|P-value:4.78E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.26|P-value:2.69E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:-3.42|P-value:1.62E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.02|P-value:7.30E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.18|P-value:4.10E-4 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.08|P-value:5.42E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.04|P-value:6.14E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:2.93|P-value:9.15E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.10|P-value:4.88E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:-3.25|P-value:3.15E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.77|P-value:3.10E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.15|P-value:4.12E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:3.96|P-value:1.35E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:4.62|P-value:4.68E-7 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.10|P-value:5.59E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.53|P-value:1.02E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.79|P-value:5.04E-10 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.36|P-value:2.01E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.37|P-value:1.74E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.89|P-value:1.82E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.09|P-value:5.22E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-2.94|P-value:9.96E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.29|P-value:2.41E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.00|P-value:7.86E-4 ID:SGTC_2243|Compound:6634756|FD-Score:3.33|P-value:1.99E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.59|P-value:7.73E-5 ID:SGTC_2351|Compound:9026858|FD-Score:5.60|P-value:1.43E-9 ID:SGTC_2396|Compound:5342950|FD-Score:3.09|P-value:5.15E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.63|P-value:5.75E-5 ID:SGTC_2447|Compound:5741742|FD-Score:3.47|P-value:1.14E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:9.07|P-value:3.92E-22 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-4.65|P-value:4.82E-7 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:6.51|P-value:2.61E-12 ID:SGTC_2556|Compound:citropten|FD-Score:3.00|P-value:7.19E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.45|P-value:1.16E-6 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.00|P-value:7.11E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.38|P-value:1.88E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.64|P-value:5.49E-5 ID:SGTC_2864|Compound:9034755|FD-Score:-3.52|P-value:1.06E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.61|P-value:6.26E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.06|P-value:5.69E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.39|P-value:1.60E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.40|P-value:1.54E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.27|P-value:2.59E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.34|P-value:1.94E-4 ID:SGTC_3260|Compound:9137109|FD-Score:3.35|P-value:1.84E-4

Top fitness defect scores for YDL154W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2533 agelasine 9.07 13.7 µM 3.92E-22 ERG2 6 6.11E-20
2 SGTC_2536 dehydroabietamide 6.51 32.0 µM 2.61E-12 2 3.74E-11
3 SGTC_2351 9026858 5.60 72.3 µM 1.43E-9 RNA processing & uracil transport 6 1.06E-8
4 SGTC_1233 0325-0408 4.62 14.2 µM 4.68E-7 13 1.88E-6
5 SGTC_2583 asiatic acid 4.45 100.0 µM 1.16E-6 25 4.23E-6
6 SGTC_411 enrofloxacin 4.33 100.0 µM 2.19E-6 DNA damage response 6 7.48E-6
7 SGTC_1227 3909-8010 3.96 46.6 µM 1.35E-5 19 3.82E-5
8 SGTC_1908 st060353 3.89 56.5 µM 1.82E-5 RPP1 & pyrimidine depletion 39 4.99E-5
9 SGTC_1160 4092-0570 3.77 10.5 µM 3.10E-5 plasma membrane duress 43 8.04E-5
10 SGTC_624 k048-0052 3.68 28.0 µM 4.78E-5 Golgi 22 1.19E-4
11 SGTC_2736 butenafine 3.64 14.7 µM 5.49E-5 36 1.34E-4
12 SGTC_2437 5741531 3.63 144.2 µM 5.75E-5 49 1.40E-4
13 SGTC_2959 9079155 3.61 11.0 µM 6.26E-5 38 1.51E-4
14 SGTC_163 1327-0036 3.50 7.5 µM 1.02E-4 143 2.35E-4
15 SGTC_2447 5741742 3.47 33.8 µM 1.14E-4 39 2.59E-4
16 SGTC_159 3970-0959 3.40 50.0 µM 1.54E-4 116 3.39E-4
17 SGTC_3103 9120822 3.40 49.5 µM 1.54E-4 31 3.40E-4
18 SGTC_3049 9092223 3.39 49.5 µM 1.60E-4 42 3.51E-4
19 SGTC_114 0364-0001 3.37 490.3 µM 1.70E-4 75 3.71E-4
20 SGTC_1780 st038448 3.37 24.5 µM 1.74E-4 iron homeostasis 43 3.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.218 2.24E-37 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.153 6.42E-19 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.148 7.91E-18 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.142 1.27E-16 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.140 3.20E-16 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.135 3.31E-15 YNL339C YRF1-6 hom Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.135 4.38E-15 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.134 7.95E-15 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.127 1.51E-13 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.126 2.60E-13 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.119 4.15E-12 YFR019W FAB1 hom 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
0.119 4.07E-12 YER028C MIG3 hom Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.119 4.20E-12 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.115 2.25E-11 YIL155C GUT2 hom Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner
0.114 3.33E-11 YOR266W PNT1 hom Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine