YDL160C-A / MHF2

Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL160C-A / MHF2

Click on Significant Values for Screen Details ID:SGTC_85|Compound:0868-0259|FD-Score:3.36|P-value:5.38E-4 ID:SGTC_106|Compound:0092-0003|FD-Score:3.59|P-value:2.36E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.18|P-value:9.87E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.57|P-value:2.57E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.27|P-value:7.31E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.45|P-value:3.94E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.33|P-value:1.07E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:5.67|P-value:1.60E-8 ID:SGTC_389|Compound:tpck|FD-Score:-4.50|P-value:5.05E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.62|P-value:1.92E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.39|P-value:4.80E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.52|P-value:3.08E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.26|P-value:7.42E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.38|P-value:5.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.31|P-value:6.26E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:8.93|P-value:1.43E-18 ID:SGTC_954|Compound:1189-0853|FD-Score:4.32|P-value:1.26E-5 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.21|P-value:8.13E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.62|P-value:2.13E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.40|P-value:4.59E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.20|P-value:2.08E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.97|P-value:5.45E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.32|P-value:6.22E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.22|P-value:8.61E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.64|P-value:1.75E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.35|P-value:5.49E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.17|P-value:9.10E-4 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.27|P-value:7.17E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.35|P-value:5.07E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.83|P-value:9.52E-5 ID:SGTC_1660|Compound:st012960|FD-Score:3.24|P-value:8.08E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.53|P-value:2.92E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.31|P-value:5.71E-4 ID:SGTC_1806|Compound:st050005|FD-Score:4.05|P-value:4.00E-5 ID:SGTC_1811|Compound:st050069|FD-Score:3.57|P-value:2.51E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.22|P-value:8.64E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.49|P-value:3.02E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-3.35|P-value:5.02E-4 ID:SGTC_2035|Compound:5154463|FD-Score:3.21|P-value:8.79E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.60|P-value:2.24E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.71|P-value:1.49E-4 ID:SGTC_2320|Compound:7373003|FD-Score:5.47|P-value:4.69E-8 ID:SGTC_2324|Compound:6149169|FD-Score:3.26|P-value:7.45E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.33|P-value:5.32E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.25|P-value:1.73E-5 ID:SGTC_2825|Compound:7998094|FD-Score:3.23|P-value:8.22E-4 ID:SGTC_2829|Compound:7992743|FD-Score:4.12|P-value:2.98E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-3.22|P-value:7.83E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.53|P-value:2.89E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.35|P-value:1.12E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.91|P-value:7.09E-5 ID:SGTC_3217|Compound:9129673|FD-Score:4.41|P-value:8.65E-6 ID:SGTC_3251|Compound:9135047|FD-Score:3.21|P-value:8.75E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.34|P-value:5.64E-4 ID:SGTC_3307|Compound:9126885|FD-Score:3.81|P-value:1.01E-4 ID:SGTC_85|Compound:0868-0259|FD-Score:3.36|P-value:5.38E-4 ID:SGTC_106|Compound:0092-0003|FD-Score:3.59|P-value:2.36E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.18|P-value:9.87E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.57|P-value:2.57E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.27|P-value:7.31E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.45|P-value:3.94E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.33|P-value:1.07E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:5.67|P-value:1.60E-8 ID:SGTC_389|Compound:tpck|FD-Score:-4.50|P-value:5.05E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.62|P-value:1.92E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.39|P-value:4.80E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.52|P-value:3.08E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.26|P-value:7.42E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.38|P-value:5.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.31|P-value:6.26E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:8.93|P-value:1.43E-18 ID:SGTC_954|Compound:1189-0853|FD-Score:4.32|P-value:1.26E-5 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.21|P-value:8.13E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.62|P-value:2.13E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.40|P-value:4.59E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.20|P-value:2.08E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.97|P-value:5.45E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.32|P-value:6.22E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.22|P-value:8.61E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.64|P-value:1.75E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.35|P-value:5.49E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.17|P-value:9.10E-4 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.27|P-value:7.17E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.35|P-value:5.07E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.83|P-value:9.52E-5 ID:SGTC_1660|Compound:st012960|FD-Score:3.24|P-value:8.08E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.53|P-value:2.92E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-3.31|P-value:5.71E-4 ID:SGTC_1806|Compound:st050005|FD-Score:4.05|P-value:4.00E-5 ID:SGTC_1811|Compound:st050069|FD-Score:3.57|P-value:2.51E-4 ID:SGTC_1854|Compound:st056233|FD-Score:3.22|P-value:8.64E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.49|P-value:3.02E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-3.35|P-value:5.02E-4 ID:SGTC_2035|Compound:5154463|FD-Score:3.21|P-value:8.79E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.60|P-value:2.24E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.71|P-value:1.49E-4 ID:SGTC_2320|Compound:7373003|FD-Score:5.47|P-value:4.69E-8 ID:SGTC_2324|Compound:6149169|FD-Score:3.26|P-value:7.45E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-3.33|P-value:5.32E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.25|P-value:1.73E-5 ID:SGTC_2825|Compound:7998094|FD-Score:3.23|P-value:8.22E-4 ID:SGTC_2829|Compound:7992743|FD-Score:4.12|P-value:2.98E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-3.22|P-value:7.83E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.53|P-value:2.89E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.35|P-value:1.12E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.91|P-value:7.09E-5 ID:SGTC_3217|Compound:9129673|FD-Score:4.41|P-value:8.65E-6 ID:SGTC_3251|Compound:9135047|FD-Score:3.21|P-value:8.75E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.34|P-value:5.64E-4 ID:SGTC_3307|Compound:9126885|FD-Score:3.81|P-value:1.01E-4

Top fitness defect scores for YDL160C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_953 1188-0045 8.93 9.7 µM 1.43E-18 60S ribosome export 1 2.19E-19
2 SGTC_385 1494-0393 5.67 12.4 µM 1.60E-8 3 7.21E-9
3 SGTC_2320 7373003 5.47 200.0 µM 4.69E-8 6 2.22E-8
4 SGTC_3217 9129673 4.41 49.5 µM 8.65E-6 23 5.21E-6
5 SGTC_2970 9090938 4.35 41.9 µM 1.12E-5 RNA processing & uracil transport 28 6.85E-6
6 SGTC_954 1189-0853 4.32 2.9 µM 1.26E-5 endomembrane recycling 30 7.71E-6
7 SGTC_2772 vinpocetine 4.25 57.1 µM 1.73E-5 18 1.08E-5
8 SGTC_968 1309-0633 4.20 340.0 µM 2.08E-5 9 1.31E-5
9 SGTC_2829 7992743 4.12 58.4 µM 2.98E-5 30 1.90E-5
10 SGTC_1806 st050005 4.05 60.3 µM 4.00E-5 7 2.59E-5
11 SGTC_1099 5-nonyloxytryptamine 3.97 2.9 µM 5.45E-5 20 3.58E-5
12 SGTC_2989 9073649 3.91 71.4 µM 7.09E-5 42 4.71E-5
13 SGTC_1607 st000305 3.83 50.6 µM 9.52E-5 16 6.41E-5
14 SGTC_3307 9126885 3.81 69.3 µM 1.01E-4 37 6.83E-5
15 SGTC_2249 haloperidol 3.71 116.4 µM 1.49E-4 ergosterol depletion effects on membrane 130 1.02E-4
16 SGTC_964 1287-0107 3.62 19.4 µM 2.13E-4 cell wall 59 1.49E-4
17 SGTC_2248 tofa 3.60 1.1 µM 2.24E-4 sphingolipid biosynthesis & PDR1 48 1.57E-4
18 SGTC_106 0092-0003 3.59 11.6 µM 2.36E-4 copper-dependent oxidative stress 22 1.66E-4
19 SGTC_1811 st050069 3.57 61.5 µM 2.51E-4 8 1.77E-4
20 SGTC_236 1509-0011 3.57 5.3 µM 2.57E-4 409 1.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 1.25E-14 YJL203W PRP21 het Subunit of the SF3a splicing factor complex, required for spliceosome assembly
0.108 2.99E-10 YOL123W HRP1 het Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
0.101 4.50E-9 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.099 1.00E-8 YLR126C YLR126C hom Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.097 1.80E-8 YCL057C-A MOS1 hom Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1
0.097 1.87E-8 YDR332W IRC3 hom Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.092 8.42E-8 YHL005C_d YHL005C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W
0.092 9.96E-8 YDL051W LHP1 hom RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
0.091 1.21E-7 YKL208W CBT1 hom Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
0.090 1.69E-7 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.090 1.97E-7 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.088 3.11E-7 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.087 3.91E-7 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.086 6.74E-7 YEL026W SNU13 het RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
0.086 6.77E-7 YBR085C-A YBR085C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress