YDL166C / FAP7

Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL166C / FAP7

Click on Significant Values for Screen Details ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.66|P-value:2.86E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-4.05|P-value:6.74E-5 ID:SGTC_300|Compound:r073-0011|FD-Score:3.30|P-value:9.43E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.66|P-value:2.81E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:4.78|P-value:3.36E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.41|P-value:6.53E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:-3.37|P-value:7.32E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.91|P-value:1.13E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.52|P-value:4.44E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.96|P-value:9.31E-5 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.61|P-value:3.37E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.41|P-value:6.55E-4 ID:SGTC_1758|Compound:st042942|FD-Score:-4.47|P-value:1.26E-5 ID:SGTC_1969|Compound:st069479|FD-Score:-3.34|P-value:8.14E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.67|P-value:2.69E-4 ID:SGTC_2148|Compound:5546263|FD-Score:-3.44|P-value:5.97E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.37|P-value:7.47E-4 ID:SGTC_2239|Compound:6685329|FD-Score:6.37|P-value:9.83E-10 ID:SGTC_2245|Compound:6637752|FD-Score:4.61|P-value:7.05E-6 ID:SGTC_2261|Compound:9010538|FD-Score:4.21|P-value:3.61E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:4.58|P-value:8.06E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.59|P-value:3.62E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.13|P-value:5.04E-5 ID:SGTC_2832|Compound:7992762|FD-Score:4.82|P-value:2.72E-6 ID:SGTC_2843|Compound:9000894|FD-Score:-3.38|P-value:7.20E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.72|P-value:4.24E-6 ID:SGTC_2998|Compound:9066762|FD-Score:3.40|P-value:6.83E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.43|P-value:6.07E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-3.32|P-value:8.73E-4 ID:SGTC_3220|Compound:9131269|FD-Score:3.63|P-value:3.12E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.57|P-value:3.82E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.66|P-value:2.86E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-4.05|P-value:6.74E-5 ID:SGTC_300|Compound:r073-0011|FD-Score:3.30|P-value:9.43E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.66|P-value:2.81E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:4.78|P-value:3.36E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.41|P-value:6.53E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:-3.37|P-value:7.32E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.91|P-value:1.13E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.52|P-value:4.44E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:-3.96|P-value:9.31E-5 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.61|P-value:3.37E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.41|P-value:6.55E-4 ID:SGTC_1758|Compound:st042942|FD-Score:-4.47|P-value:1.26E-5 ID:SGTC_1969|Compound:st069479|FD-Score:-3.34|P-value:8.14E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.67|P-value:2.69E-4 ID:SGTC_2148|Compound:5546263|FD-Score:-3.44|P-value:5.97E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.37|P-value:7.47E-4 ID:SGTC_2239|Compound:6685329|FD-Score:6.37|P-value:9.83E-10 ID:SGTC_2245|Compound:6637752|FD-Score:4.61|P-value:7.05E-6 ID:SGTC_2261|Compound:9010538|FD-Score:4.21|P-value:3.61E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:4.58|P-value:8.06E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.59|P-value:3.62E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.13|P-value:5.04E-5 ID:SGTC_2832|Compound:7992762|FD-Score:4.82|P-value:2.72E-6 ID:SGTC_2843|Compound:9000894|FD-Score:-3.38|P-value:7.20E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.72|P-value:4.24E-6 ID:SGTC_2998|Compound:9066762|FD-Score:3.40|P-value:6.83E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.43|P-value:6.07E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-3.32|P-value:8.73E-4 ID:SGTC_3220|Compound:9131269|FD-Score:3.63|P-value:3.12E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.57|P-value:3.82E-4

Top fitness defect scores for YDL166C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2239 6685329 6.37 187.6 µM 9.83E-10 1 9.68E-11 1.29
2 SGTC_2832 7992762 4.82 52.0 µM 2.72E-6 5 7.01E-7 0.87
3 SGTC_755 1995-0008 4.78 37.3 µM 3.36E-6 1 8.89E-7 0.65
4 SGTC_2245 6637752 4.61 200.0 µM 7.05E-6 2 2.04E-6 0.17
5 SGTC_2633 5,7-dihydroxyisoflavone 4.58 100.0 µM 8.06E-6 3 2.37E-6 0.62
6 SGTC_2261 9010538 4.21 184.7 µM 3.61E-5 3 1.27E-5 0.26
7 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 4.13 100.0 µM 5.04E-5 4 1.84E-5 0.03
8 SGTC_560 2332-1943 3.66 155.0 µM 2.81E-4 5 1.26E-4 0.12
9 SGTC_219 mitoxanthrone 3.66 164.4 µM 2.86E-4 DNA intercalators 15 1.28E-4 0.03
10 SGTC_3220 9131269 3.63 49.5 µM 3.12E-4 4 1.42E-4 0.24
11 SGTC_1438 4092-1045 3.61 4.0 µM 3.37E-4 5 1.54E-4 0.26
12 SGTC_2634 4'-methoxyflavone 3.59 100.0 µM 3.62E-4 11 1.68E-4 0.02
13 SGTC_3223 9129970 3.57 49.5 µM 3.82E-4 9 1.78E-4 0.18
14 SGTC_1072 oxiconazole 3.41 14.9 nM 6.53E-4 ergosterol depletion effects on membrane 27 3.23E-4 0.02
15 SGTC_2998 9066762 3.40 71.4 µM 6.83E-4 RPP1 & pyrimidine depletion 14 3.40E-4 0.02
16 SGTC_2191 5911152 3.37 200.0 µM 7.47E-4 6 3.76E-4 0.04
17 SGTC_300 r073-0011 3.30 5.0 µM 9.43E-4 5 4.88E-4 0.05
18 SGTC_796 1234-0020 3.24 70.7 µM 0.00113 SWF1 & branched chain AA biosynthesis 13 5.99E-4 0.09
19 SGTC_347 4534-1311 3.20 26.2 µM 0.00126 RPP1 & pyrimidine depletion 8 6.77E-4 0.08
20 SGTC_2537 larixol 3.16 68.9 µM 0.00146 16 7.93E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 3.10E-9 YER176W ECM32 hom DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
0.094 4.89E-8 YDR428C BNA7 hom Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
0.085 8.82E-7 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.084 9.86E-7 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.083 1.39E-6 YJR036C HUL4 hom Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex
0.076 9.82E-6 YJR035W RAD26 hom Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
0.074 1.83E-5 YJR131W MNS1 hom Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
0.073 2.29E-5 YIL159W BNR1 hom Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1
0.070 4.79E-5 YER075C PTP3 hom Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm
0.068 8.32E-5 YDL230W PTP1 hom Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
0.068 8.78E-5 YOL013C HRD1 hom Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
0.067 9.78E-5 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.067 1.02E-4 YDR261C EXG2 hom Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.065 1.76E-4 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.064 2.01E-4 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination