Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YDL166C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.102 | 3.10E-9 | YER176W | ECM32 | hom | DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes |
0.094 | 4.89E-8 | YDR428C | BNA7 | hom | Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine |
0.085 | 8.82E-7 | YGR185C | TYS1 | het | Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress |
0.084 | 9.86E-7 | YJR115W_p | YJR115W_p | hom | Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication |
0.083 | 1.39E-6 | YJR036C | HUL4 | hom | Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex |
0.076 | 9.82E-6 | YJR035W | RAD26 | hom | Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein |
0.074 | 1.83E-5 | YJR131W | MNS1 | hom | Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation |
0.073 | 2.29E-5 | YIL159W | BNR1 | hom | Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 |
0.070 | 4.79E-5 | YER075C | PTP3 | hom | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm |
0.068 | 8.32E-5 | YDL230W | PTP1 | hom | Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation |
0.068 | 8.78E-5 | YOL013C | HRD1 | hom | Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon |
0.067 | 9.78E-5 | YPL262W | FUM1 | hom | Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria |
0.067 | 1.02E-4 | YDR261C | EXG2 | hom | Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor |
0.065 | 1.76E-4 | YBR067C | TIP1 | hom | Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins |
0.064 | 2.01E-4 | YGL110C | CUE3 | hom | Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination |