YDL182W / LYS20

Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL182W / LYS20

Click on Significant Values for Screen Details ID:SGTC_793|Compound:0488-0545|FD-Score:2.77|P-value:8.74E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.10|P-value:2.30E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-3.27|P-value:1.01E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:5.84|P-value:1.91E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:-4.76|P-value:3.24E-8 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.20|P-value:1.46E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.73|P-value:1.22E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.05|P-value:2.79E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.16|P-value:1.25E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:-2.90|P-value:4.79E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.31|P-value:8.35E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.32|P-value:8.74E-5 ID:SGTC_1722|Compound:st034304|FD-Score:-3.31|P-value:8.31E-5 ID:SGTC_1739|Compound:st037196|FD-Score:3.49|P-value:3.92E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.25|P-value:1.21E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-4.12|P-value:1.37E-6 ID:SGTC_1922|Compound:5578187|FD-Score:2.95|P-value:4.27E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.34|P-value:7.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.53|P-value:3.29E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-2.82|P-value:6.55E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.86|P-value:6.14E-4 ID:SGTC_2088|Compound:5278280|FD-Score:2.87|P-value:6.00E-4 ID:SGTC_2799|Compound:7812892|FD-Score:2.90|P-value:5.18E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.30|P-value:9.71E-5 ID:SGTC_3166|Compound:9102297|FD-Score:2.79|P-value:7.99E-4 ID:SGTC_3172|Compound:9105863|FD-Score:-3.35|P-value:6.95E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.06|P-value:2.75E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:2.77|P-value:8.74E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.10|P-value:2.30E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-3.27|P-value:1.01E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:5.84|P-value:1.91E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:-4.76|P-value:3.24E-8 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.20|P-value:1.46E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.73|P-value:1.22E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.05|P-value:2.79E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.16|P-value:1.25E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:-2.90|P-value:4.79E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.31|P-value:8.35E-5 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.32|P-value:8.74E-5 ID:SGTC_1722|Compound:st034304|FD-Score:-3.31|P-value:8.31E-5 ID:SGTC_1739|Compound:st037196|FD-Score:3.49|P-value:3.92E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.25|P-value:1.21E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-4.12|P-value:1.37E-6 ID:SGTC_1922|Compound:5578187|FD-Score:2.95|P-value:4.27E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.34|P-value:7.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.53|P-value:3.29E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-2.82|P-value:6.55E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.86|P-value:6.14E-4 ID:SGTC_2088|Compound:5278280|FD-Score:2.87|P-value:6.00E-4 ID:SGTC_2799|Compound:7812892|FD-Score:2.90|P-value:5.18E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.30|P-value:9.71E-5 ID:SGTC_3166|Compound:9102297|FD-Score:2.79|P-value:7.99E-4 ID:SGTC_3172|Compound:9105863|FD-Score:-3.35|P-value:6.95E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.06|P-value:2.75E-4

Top fitness defect scores for YDL182W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_807 1315-0151 5.84 48.0 µM 1.91E-11 5 2.63E-9
2 SGTC_1204 4049-0315 4.16 18.3 µM 1.25E-6 22 1.59E-5
3 SGTC_1039 k821-0043 3.73 94.0 µM 1.22E-5 Golgi 31 9.54E-5
4 SGTC_1926 methyl fluorone black 3.53 53.6 µM 3.29E-5 DNA intercalators 77 2.09E-4
5 SGTC_1739 st037196 3.49 92.5 µM 3.92E-5 38 2.40E-4
6 SGTC_1599 8-chlro-1-tetrahydronorharmanone 3.32 90.6 µM 8.74E-5 56 4.52E-4
7 SGTC_2807 7945238 3.30 52.0 µM 9.71E-5 57 4.91E-4
8 SGTC_1820 st053765 3.25 18.6 µM 1.21E-4 RPP1 & pyrimidine depletion 45 5.83E-4
9 SGTC_1035 k081-0041 3.20 55.2 µM 1.46E-4 Golgi 51 6.78E-4
10 SGTC_797 k781-0471 3.10 270.0 µM 2.30E-4 RPP1 & pyrimidine depletion 55 9.71E-4
11 SGTC_3334 9142812 3.06 21.9 µM 2.75E-4 63 0.00112
12 SGTC_1140 3229-1848 3.05 3.0 µM 2.79E-4 31 0.00113
13 SGTC_1922 5578187 2.95 25.0 µM 4.27E-4 TRP & mitochondrial translation 90 0.00159
14 SGTC_2799 7812892 2.90 81.8 µM 5.18E-4 superoxide 80 0.00185
15 SGTC_2088 5278280 2.87 127.4 µM 6.00E-4 90 0.00208
16 SGTC_2057 5236571 2.86 38.3 µM 6.14E-4 RPP1 & pyrimidine depletion 102 0.00212
17 SGTC_3166 9102297 2.79 49.5 µM 7.99E-4 Golgi 93 0.00261
18 SGTC_793 0488-0545 2.77 106.0 µM 8.74E-4 78 0.00280
19 SGTC_2786 7743617 2.71 45.5 µM 0.00111 90 0.00340
20 SGTC_1368 1852-0141 2.65 284.0 µM 0.00137 ERG2 119 0.00400

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.41E-26 YNR046W TRM112 het Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
0.177 6.12E-25 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.142 1.11E-16 YDR487C RIB3 het 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
0.140 4.37E-16 YOR172W YRM1 hom Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
0.127 1.30E-13 YBR263W SHM1 hom Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
0.117 1.04E-11 YGR198W YPP1 het Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
0.116 1.97E-11 YKL060C FBA1 het Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
0.107 5.37E-10 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.102 3.16E-9 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.096 2.34E-8 YGR117C_p YGR117C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.096 2.66E-8 YER001W MNN1 hom Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
0.093 6.00E-8 YMR295C YMR295C hom Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress
0.092 1.08E-7 YGL021W ALK1 hom Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins
0.091 1.27E-7 YDL172C_d YDL172C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.089 2.10E-7 YKL137W CMC1 hom Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif